Abstract:

Objective To investigate the effect of thrombospondin-2（THBS2） on proliferation，invasion and migration of human cervical squamous carcinoma SiHa Cells and the ability of tumor formation in nude mice. Methods Human cervical squamous  carcinoma SiHa cells transfected with the THBS2 overexpression  lentivirus served as the experimental group（THBS2-LV group），transfected with empty virus served as a negative control group （NC group） and untreated human cervical squamous  carcinoma SiHa cells served as a blank control group.The mRNA and THBS2 protein expression were detected by RT-qPCR and Western blot respectively. The proliferation and apoptotic rate of cells were assayed by CCK-8 method and flow cytometry respectively. Cell invasion and migration were analyzed by Transwell assay and Scratch test. The tumor formation ability of nude mice was observed by tumor formation experiment in nude mice. Results Compared with the NC group and the blank control group，the expression of THBS2 mRNA and protein and the apoptotic rate of cells increased in THBS2-LV group，while the cell proliferation，invasion and migration were reduced（P<0.05）. The results of tumor formation in nude mice showed that the subcutaneous tumorigenic ability and average tumor volume of nude mice inoculated with THBS2-LV group were lower than those of nude mice inoculated with NC group and blank control group（P<0.01）. Conclusions Overexpression of THBS2 can inhibit the proliferation，invasion，migration and subcutaneous tumor formation of cervical squamous carcinoma SiHa cells. Up-regulation of THBS2 can inhibit the development of cervical squamous cell carcinoma.

Key words: Uterus neoplasms, Cervical squamous cell carcinoma, SiHa cells, Thrombospondin-2, Proliferation, Apoptosis, Vitro experi-ment, Animal experiment