Abstract:

Objective To investigate the effect of corydalis saxicola bunting aqueous extract on hepatocellular carcinoma HepG2 proliferation and migration and its possible mechanism. Methods The culture medium containing different concentrations（0.4 mg/mL 0.8 mg/mL 1.6 mg/mL） of corydalis saxicola bunting aqueous extract was applied to hepatocellular carcinoma HepG2 cells. The proliferation of HepG2 cells was assayed using the cell counting kit-8（CCK-8），and invasion was detected using the Transwell test. The expression of NF-κBp65 was detected by real-time PCR and Western blot analysis. Results The results of CCK-8 showed that the inhibition rate of HepG2 cells in different concentra-tions of corydalis saxicola bunting aqueous extract at 24 h，48 h，and 72 h was statistically significant （P<0.05），and the cell prolifer-ation in-hibition rate of 0.8 mg/mL and 1.6 mg/mL was higher than 0.4 mg/mL（P<0.05）. The results of Transwell experiments showed that the cell migration of HepG2 cells treated with different concentrations of Rhizoma Coptidis aqueous extracts for 48 h was lower than that of the control group （P<0.05），and the expression of NF-Bp65 protein and mRNA was up-regulated （P<0.05）. Conclusions Corydalis saxicola bunting aqueous extract inhibits proliferation，migration of HepG2，and the mechanism of it may do so by up-regulating NF-κBp65 expression.

Key words: Liver neoplasm, Corydalis saxicola buntingaqueous extract, HepG2, ；Proliferation, Migration, NF-κBp65