Abstract:

 Objective To investigate the effects of portulaca oleracea polysaccharide on the proliferation and apoptosisof gastric cancer SGC7901 cells. Methods Gastric cancer SGC7901 cells were cultured in vitro and treated with differentconcentrations of portulaca oleracea polysaccharides（25 μg/mL，50 μg/mL，100 μg/mL，200 μg/mL，300 μg/mL）. Normalsaline was used as the negative control group and 5-FU as the positive control group. The cell proliferation was detectedby MTT assay，and apoptosis was detected by flow cytometry. Results The proliferation inhibition rate of SGC7901 cellstreated with portulaca oleracea polysaccharide at different concentrations increased with the increases of duration timeand drug concentration. After 48 h，the proliferation inhibition rates of 200 μg/mL and 300 μg/mL portulaca oleracea polysaccharide groups were（53.02±2.46）%，（57.20±2.14）%，respectively，which were comparable to those of the 5-FU group，（56.36±3.26）% （P>0.05）. The apoptosis rates of each concentration group were significantly increased compared with the negative control group，followed by 31.92%，38.39%，40.45%，48.49%，55.71%（all P<0.05）. Compared with the 5-FU group，the apoptosis rates of low-concentration（25 μg/mL，50 μg/mL，and 100 μg/mL） groups were decreased（P<0.05）， while the apoptosis rate was increased in the high concentration（300 μg/mL） group（P<0.05）. Conclusions Portulaca oleracea polysaccharide can inhibit the proliferation and induce the apoptosis of gastric cancer SGC7901 cells. Portulaca oleracea polysaccharide can inhibit the proliferation and induce the apoptosis of gastric cancer SGC7901 cells.

Key words: Gastric cancer, Portulaca oleracea polysaccharide, Proliferation, Apoptosis