Abstract: Objective  To explore and identify the preparation methods of ovarian cancer organoid model. Methods The primary focal tumor tissue of 3 ovarian cancer patients undergoing surgery in Guangxi Medical University Cancer Hospital from 2019 to 2020 were selected, disintegrate into cell suspension and coated with Matrigel, then cultured for 2 weeks to form a single diameter of over 100 μm globule to digested and passed, the globule were embedded in paraffin and slices when cultured to the second generation. After decolorization, the HE staining and immunohistochemistry were performed and compared with the original tissue for histological and cytological evaluation. Results The transpassable cryopreservation organoid was constructed. The results of HE staining showed that the organoid was highly similar to the original tissue in adenocarcinoma structure. The results of immunohistochemistry showed that the expression of p53, PAX-8, WT-1 and CK7 markers were basically the same between the original tissue and the organoid. Conclusions Ovarian cancer organoids with Matrigel coated and infiltration medium can be successfully constructed the ovarian cancer organoids that basically consistent with the original tissue in tissue and cell level identification results.

Key words: Ovarian cancer, Organoid, Preparation method, Pathological identification