Abstract: Objective To explore a proper anesthesia method for laryngeal surgery on experimental dogs.Methods  Thirteen adult dogs undergoing total laryngectomy and laryngeal function reconstruction were divided randomly into two groups.Animals in group A were treated with 3% pentobarbital sodium (1ml/kg，intraperitoneal injection) only.Animals in group B were treated with 5% pentobarbital sodium (0.25ml/kg，intramuscular injection) combined with Sumianxin II (0.05ml/kg，intramuscular injection).Results The treatment in group A showed the anesthesia induction time was（19.7±6.2）min，the maintenance of first anesthesia time was（119.6±34.6）min.It needed several more injections to keep anesthesia during the surgery and had poor postoperative recovery.The treatment in group B showed the induction time was（5.5±1.8）min，the maintenance of anesthesia time was（112.5±24.3）min.Only one more injection with 1/2 dose of the first infection was needed，which prolonged the maintenance of anesthesia time to at least（169.8±20.2）min.The dogs in this group reanimated soon after operation.Between group A and group B，the induction time was significantly different (P＜0.05) while the maintenance time of first anesthesia was not significantly different (P＞0.05).Both of the times of additional injection and the ratio of actual amount vs suggested amount of pentobarbital were significantly different (P＜0.05)Conclusions Pentobarbital sodium combined with Sumianxin appears to be a satisfactory method to anaesthetize dogs for the operation of total laryngectomy and laryngeal function reconstruction.It can decrease induction time，reduce the time and dose of additional anesthetics，and obtain more effective anesthesia and rapid recovery.

Key words: Pentobarbital Sodium, Sumianxin, Anesthesia, Dog, Laryngeal Carcinoma