Objective To construct a lentivirus expression vector to drive stable Cat S overexpression in the hepatocellular carcinoma （HCC）cell line MHCC97H，and to investigate the effects of overexpression on cell proliferation and migration. Methods The Cat S gene was inserted into the lentiviral expression vector pLVX-EGFP-3FLAG-Puro using Age I and EcoR I restriction enzymes and transfected into 293T cells to generate recombinant lentivirus. This virus was used to infect MHCC97H cells，and positive cells were selected using puromycin. Real-time quantitative PCR，Western blots，MTT assay，the transwell migration assay and the wound healing migration assay were used to assess the effects of Cat S overexpression on proliferation and migration of MHCC97H cells. Results A lentiviral vector containing the Cat S gene was constructed， and an MHCC97H cell line stably overexpressing Cat S was established. Cat S overexpression was associated with significantly shorter cell doubling time，as well as significantly greater invasion and migration abilities. Conclusions Lentivirus-mediated Cat S overexpression can increase MHCC97H proliferationand migration， opening the door to future studies of molecular mechanisms of liver cancer invasion and metastasis.