Abstract:

Objective To investigate the effect of Linc00704 （long non-coding RNA 00704） on the proliferation，migration and invasion ability of colorectal cancer cells. Methods RT-qPCR was used to detect the expression of Linc00704 mRNA in the matched colorectal cancer and adjacent normal tissues，colorectal cancer cells and normal immortalized human colon epithelial cell line FHC. A low-expressing cell model of Linc00704 was constructed by antisense oligonucleotide（ASO）transfecting colorectal cancer cells，and a cell model with overexpression of Linc00704 was constructed by lentivirus infecting colorectal cancer cells. The transfection efficiency was detected by RT-qPCR，the cell proliferation was detected by CKK-8 assay，and the cell migration and invasion were measured by Transwell assay. Results Compared with the adjacent tissues，the expression of Linc00704 decreased in colorectal cancer tissues（t=4.103，P<0.001）. Among the 8 colorectal cancer cell lines，the expression of Linc00704 in 6 cell lines（RKO，HCT15，NCI-H716，SW480，SW1463，SW48）was lower than that of FHC（F=44.750，P<0.001）. Silencing the expression of Linc00704 could promote the migration and invasion of Caco-2 and DLD-1 cells （all P<0.01），but had no significant effect on the proliferation （all P>0.05）. The overexpression of Linc00704 could inhibit the migration and invasion of RKO and HCT15 cells（all P<0.001），but had no effect on the proliferation（all P>0.05）. Conclusions Linc00704 is low-expressed in colorectal cancer tissues，and overexpression of Linc00704 can inhibit the migration and invasion ability of colorectal cancer cells.

Key words: Colorectal cancer, Linc00704, Proliferation, Invasion, Migration