Abstract: Objective  To investigate the clinical efficacy of intraoperative contrast⁃assisted resection of glioma with low⁃dose sodium fluorescein. Methods The clinical data of 100 patients with glioma who underwent surgical treatment in the Department of Neurosurgery, Guangxi Medical University Cancer Hospital from October 2018 to January 2021 were retrospectively analyzed. The patients were divided into the observation and the control groups according to different surgical methods, by which 47 patients in the observation group underwent microsurgical glioma resection under the guidance of low⁃dose sodium fluorescein (1 mg/kg), and 53 patients in the control group underwent glioma resection under the conventional microscope. The two groups were compared with respect to total resection rate, Karnofsky score (KPS score) at 3 months postoperatively, operative time, intraoperative bleeding, and postoperative complications. Results The surgery was successfully completed in both groups, and the total resection rate in the observation group was 80.9%, which was significantly higher than 58.5% in the control group (P=0.028). After correcting for possible confounding factors, the total resection rate of the observation group was 6.94 times higher than that of the control group (OR=6.94, 95%CI: 1.96-24.65, P=0.003); the KPS score of the observation group at 3 months after surgery was 7.94 points higher than of the control group (β=7.94, P=0.037). There was no significant differences in the mean operative time and median intraoperative bleeding between the two groups (all P>0.05). The main complications in both groups were intracranial infection and postoperative bleeding, and the incidence was not significantly different (all P>0.05). Conclusions Compared with conventional microscopic glioma resection, microscopic resection under low⁃dose sodium fluorescein guidance can improve the total resection rate of gliomas and improve the postoperative life quality of patients.

Key words: Glioma, Fluorescence guided technique, Sodium fluorescein, Microsurgery