Abstract: Objective To explore the feasibility of reversing multidrug resistance by RNA interference targeted on hypoxia inducible factor 1α(HIF-1α) gene in ovarian cancer cells.Methods Small hairpin RNA eukaryotic expression vector targeted on HIF-1α gene was constructed and transfected into COC1/DDP cells with liposome.The mRNA and protein expressions of HIF-1α and MDR-1 were measured by real time PCR and Western blot.The multidrug resistant to anticancer agent Cisplatin was evaluated by MTT assay.Results After transfected with the small hairpin RNA eukaryotic expression vector targeted on HIF-1α gene,the mRNA and protein expressions of HIF-1α and MDR-1 in COC1/DDP cells decreased significantly under hypoxic condition.Furthermore,the sensitivity to Cisplatin significantly increased.Conclusions The small hairpin RNA eukaryotic expression vector targeted on HIF-1α gene can effectively suppress the expressions of HIF-1α and MDR-1 genes in ovarian cancer cells,and then can reverse the multidrug resistance.

Key words: Ovarian cancer, Drug tolerance, Hypoxia-inducible factor-1α, RNA interference