Abstract: Objective To study the expression ofβ-amyloid A4 protein(Amyloid beta A4 protein,APP) mRNA in ovarian tumor tissues and its clinical significance.Methods One-step Trizol extraction method was used to extract the total RNA from 49 cases of ovarian cancer,20 cases of benign ovarian tumor and 16 cases of normal ovarian tissue.Real-time fluorescence quantitative PCR technology was used to analyze APP mRNA in each sample.Results The expression of APP mRNA in ovarian cancer tissue was significantly higher than those in benign ovarian tumor and normal ovarian tissue(P<0.05 ).The expression of APP mRNA in ovarian granulose cell tumor was significantly higher than that in epithelial ovarian cancer(P<0.05).The expression of APP mRNA in ovarian cancer tissue from the patients treated with preoperative neo-adjuvant chemotherapy was significantly higher than that in benign ovarian tumor,but was not different from that in ovarian cancer without neo-adjuvant chemotherapy. The level of APP mRNA and the survival rate of patients with ovarian cancer had no significant correlation. Conclusions The high expression of APP ovarian cancer is related to the histological type of tumor,but the expression of APP is not an independent judgment factor for prognosis of ovarian cancer.Chemotherapy does not reduce the expression of APP mRNA.Detecting APP expression has certain value for diagnosis of the ovarian cancer.

Key words: Ovarian cancer, Amyloid beta A4 protein, Real-time PCR, Clinical analysis