Abstract:

Objective The aim of this study was to detect GP73 in the serum of healthy individuals and patients with hepatocellular carcinoma（HCC） using Western blotting and to assess the diagnostic sensitivity and specificity of the protein. Methods Expression of GP73 was measured in healthy and HCC individuals using Western blotting. The diagnostic performance of GP73 was evaluated using a receiver operating characteristic（ROC） curve. Results GP73 protein was expressed at significantly higher levels in HCC patients than in healthy individuals（P<0.001）. Using a GP73 concentration of 82.2 ng/mL as the diagnostic threshold for HCC，the area under the ROC curve was 0.823，sensitivity was 90% and specificity was 78%. Conclusion GP73 may be a reliable serum marker for diagnosis of HCC.

Key words: Liver neoplasm, Golgi protein-73, Serum marker, Diagnosis