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25 February 2020, Volume 12 Issue 1 Previous Issue    Next Issue

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New evidence of Cancer Evo-Dev: discovery in colorectal carcinoma and hepatocellular carcinoma DING Yibo, CHEN Yifan, CAO Guangwen 2020, 12 (1):  1-5.  doi: 10.3969/j.issn.1674-5671.2020.01.01 Abstract ( 872 )   PDF (5978KB) ( 525 )   Save Related Articles | Metrics

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Advances and prospects of genome-wide association study in hepatocellular carcinoma ZHOU Zewen, ZHANG Ruoxin, WEI Qingyi, YU Hongping 2020, 12 (1):  6-13.  doi: 10.3969/j.issn.1674-5671.2020.01.02 Abstract ( 357 )   PDF (3750KB) ( 360 )   Save Related Articles | Metrics

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Interpretation of expert consensus on the clinical application of nasopharyngeal carcinoma biomarkers XIAO Zhiqiang 2020, 12 (1):  14-20.  doi: 10.3969/j.issn.1674-5671.2020.01.03 Abstract ( 428 )   PDF (1893KB) ( 516 )   Save Related Articles | Metrics

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Effect of MCUR1 expression on the growth of ovarian cancer cells and its mechanism BAI Xiangyu, CAO Shanshan, YANG Shirong, GAO Tian, CHEN Yanqin, BAO Dengke 2020, 12 (1):  21-27.  doi: 10.3969/j.issn.1674-5671.2020.01.04 Abstract ( 297 )   PDF (17411KB) ( 78 )   Save Objective To investigate the relationship between mitochondrial calcium uniporter regulator 1（MCUR1） and the prognosis of ovarian cancer patients，and the effect of MCUR1 on the proliferation and apoptosis of ovarian cancer cells as well as its possible mechanism. Methods The data of 372 ovarian cancer patients were downloaded from the Cancer Genome Atlas （TCGA） database. The Kaplan-Meier method was used to analyze the relationship between MCUR1 and the prognosis of ovarian cancer patients. Immunohistochemistry was used to detect the expression of MCUR1 in ovarian cancer tissues. Ovarian cancer A2780 cells were transfected with MCUR1 interference fragments and overexpression plasmids（siMCUR1 group and MCUR1 group），and corresponding negative controls（siCtrl and EV group） were set respectively. qRT-PCR and Western blot assay were used to detect the effect of MCUR1 intervention. MTS method and clone formation experiment were used to detect cell proliferation. Immunohistochemical and TUNEL staining methods were used to detect proliferation and apoptosis after tumor-bearing subcutaneously in nude mice. The Western blot was used to detect proliferation and apoptosis-related molecules. Results The median overall survival of the MCUR1 high expression group was higher than that of the low expression group（53.7 months vs 41.3 months，P=0.003）. Compared with the siCtrl group，the cell proliferation ability of  the  si MCUR1 group increased（P<0.01），the growth of subcutaneous tumors in nude mice accelerated（P<0.01），Ki67 expression increased（P<0.01），and the apoptotic capacity decreased（P<0.05）； the expressions of p53，Caspase-3，and Bax protein decreased，whereas the expressions of Bcl2，Cyclin D1，and Cyclin E protein increased. Compared with the EV group，the cell proliferation ability of the MCUR1 group decreased（P<0.01），the growth of subcutaneous tumor-bearing tumors in nude mice slowed down（P<0.01），Ki67 expression decreased （P<0.01），and apoptotic capacity increased （P<0.05）； the expressions of p53，Caspase-3，and Bax proteins increased，whereas the expressions of Bcl2，Cyclin D1，and Cyclin E proteins increased. Conclusions MCUR1 regulates the proliferation and apoptosis of ovarian cancer cells by regulating the p53 pathway. MCUR1 may play a role as a tumor suppressor in ovarian cancer and become a target for diagnosis and treatment of ovarian cancer. Related Articles | Metrics

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Effect of high-fat diet on liver metastasis of colon cancer and its underlying mechanism GUO Shikui, ZHANG Jian, LI Jinyuan, BAO Weimin, ZHANG Peng, HUANG Yingguang, LING Ping, WANG Yongzhi, ZHAO Quan 2020, 12 (1):  28-33.  doi: 10.3969/j.issn.1674-5671.2020.01.05 Abstract ( 257 )   PDF (10009KB) ( 53 )   Save Objective  To investigate the effect of high-fat diet on liver metastasis of colon cancer and its underlying mechanism. Methods Xenograft models of nude mice were constructed based on the data of a total of 30 NSI third-generation immunodeficient nude mice，which were implanted with colon cancer cells DLD1 into their spleens. The xenograft nude mice were evenly divided into three groups：a control group，which was fed normal diet，a high-fat diet group，which was fed high-fat diet，and a high-fat diet+AMD3100 group，which was fed high-fat diet with intraperitoneal injection of CXCR4 antagonist AMD3100.  After 12 weeks，the nude mice in each group were sacrificed by cervical dislocation，and the weights of the bodies and livers of nude mice were monitored，and the number of tumor metastasis in the liver was counted. The protein and mRNA expression of SDF-1 and CXCR4 in tumor tissues of each group were detected by Western blot and RT-qPCR. Results After 12 weeks，the body weight，fat mass，liver weight，and serum leptin concentration of the nude mice in the high-fat diet group were significantly higher than those in the control group and the high-fat diet+AMD3100 group（P<0.05）. The proportion of liver metastasis in the control group，high-fat diet group，and high-fat diet+AMD3100 group nude mice were 30.0%，80.0%，and 40.0%，respectively. The tumor volume and the number of liver metastases in the high-fat diet group were significantly higher than those in the control group ［（3.83±0.42） mm3 vs （1.00±0.15） mm3，P<0.001； 4.33±0.58 vs 1.33±0.58，P=0.002］ and the high-fat diet+AMD3100 group ［（3.83±0.42） mm3 vs （1.96±0.15） mm3，P<0.001； 4.33±0.58 vs 2.33±0.58，P=0.002］. Western blot and RT-qPCR results showed that the protein and mRNA expression of SDF-1 and CXCR4 in colon cancer tissues in the high-fat diet group were significantly higher than those in the control group and the high-fat diet+AMD3100 group（P<0.05）. Conclusion High-fat diet can enhance liver metastasis of colon cancer by promoting the expression of SDF-1 and CXCR4 in colon cancer tissues.  Related Articles | Metrics

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MKP-1 enhancement of the sensitivity of histone deacetylase inhibitors to glioma stem cells and its underlying mechanism MENG Liang, LUO Caikui, DAI Xiaoqin, TAO Liang, LIU Shan, WANG Yuefei 2020, 12 (1):  33-38.  doi: 10.3969/j.issn.1674-5671.2020.01.06 Abstract ( 252 )   PDF (6681KB) ( 238 )   Save Objective To investigate the effect of MKP-1 on the sensitivity of histone deacetylase inhibitors（HDACi） in glioma stem cells（GSC） and its underlying mechanism. Methods A total of 53 glioma patients  from  January  2016  to December  2018  in  the Department of Neurosurgery，Third Hospital of Wuhan，were enrolled to obtain the tumors and healthy brain tissues. MKP-1 expression was detected by RT-PCR. GSC were isolated and cultured. The IC50 of HDACi MS-275 on GSC was detected by MTT assay. MKP-1 overexpressing plasmid was used to transfect cells to construct a GSC model that differentially expresses MKP-1，and MTT assay was used to analyze its sensitivity to HDACi MS-275，CCK-8 assay was used to detect cell proliferation，RT-PCR and Western blot was used to detect the expression of GSC related factors SOX2 and SOX9，and Western blot was used to detect the expression of p38，JNK and ERK1/2. Results The expression of MKP-1 in glioma tissues was significantly lower than that in healthy brain tissues（P<0.001）. The IC50 of GSC treated with HDACi MS-275 was（55.12±7.31） nmol/mL，and the cell proliferation ability was significantly lower than that in the control group（P<0.001），and the expression of MKP-1 was significantly increased（P<0.001）. The IC50 of HDACi MS-275 on MKP-1 overexpressing GSC was significantly lower than that of the control group and blank group（P<0.001）. The proliferation ability，the mRNA，and protein expression of SOX2 and SOX9 of GSC in the MKP-1 group were significantly lower than those in the control group and blank group（P<0.001）. The expression of JNK and p38 in the MKP-1 group was lower than those in the control group and blank group（P<0.001），and there was no significant difference in ERK1/2 expression（P＞0.05）. Conclusion The sensitivity of HDACi on GSC is related to the expression of MKP-1. MKP-1 may regulate the reactivity of GSC to HDACi by regulating SOX2，SOX9，p38，and JNK. Related Articles | Metrics

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Effect of Wogonin on proliferation and apoptosis of nasopharyngeal carcinoma CNE2 cells CHEN Lin, LYU Yan, HE Wenlong, HU Jing, FAN Jingying, HE Yingchun 2020, 12 (1):  39-43.  doi: 10.3969/j.issn.1674-5671.2020.01.07 Abstract ( 315 )   PDF (7606KB) ( 48 )   Save Objective To investigate the effect of Wogonin on the proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE2 and the related possible mechanism. Methods CNE2 cells were treated with Wogonin at different concentrations（2.5 μmol/L，5.0 μmol/L，10.0 μmol/L，and 20.0 μmol/L），with the solvent as the control group. The real-time unlabeled cell function analysis technique was used to monitor the proliferation of CNE2 cells，and the double fluorescent labeling method was used to detect the apoptosis of CNE2 cells，and Western blot was used to detect the protein expression of PCNA，Survivin，XIAP，and Bcl-2. Results Wogonin could inhibit the proliferation of CNE2 cells at different concentrations（2.5 μmol/L，5.0 μmol/L，10.0 μmol/L，20.0 μmol/L），and when treated for 24 h，the inhibition ratios were 20.3%，23.7%，33.4%，and 40.9%，respectively，and the IC50 was 42.41 μmol/L； when treated for 36 h，the inhibition ratios were 18.0%，22.0%，36.1%，and 40.5%，respectively，and the IC50 was 34.46 μmol/L； when treated for 48 h，the inhibition ratios were 7.4%，20.2%，35.0%，and 40.9%，respectively，and the IC50 was 22.81 μmol/L. Compared to those of the solvent control group，the apoptosis rates of CNE2 cells treated with Wogonin（10 μmol/L，20 μmol/L） increased（t=23.710，P=0.001； t=43.934，P<0.001）. When CNE2 cells were treated with 20 μmol/L Wogonin for 48 hours，the expression of Bax protein increased significantly（P<0.005），whereas the expressions of Survivin，XIAP，and Bcl-2 proteins decreased（P<0.005）. Conclusion Wogonin can inhibit the proliferation and induce the apoptosis of nasopharyngeal carcinoma CNE2 cells，probably through promoting the expression of Bax protein while inhibiting the expressions of Survivin，XIAP，and Bcl-2 proteins. Related Articles | Metrics

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Analysis of cancer incidence and mortality in Guangxi cancer registration areas,2016 LI Qiulin, CAO Ji, RONG Minhua, GE Lianying, YU Hongping, YU Jiahua 2020, 12 (1):  44-51.  doi: 10.3969/j.issn.1674-5671.2020.01.08 Abstract ( 556 )   PDF (822KB) ( 362 )   Save Objective To analyze the cancer incidence and mortality in Guangxi cancer registration areas in 2016. Methods The data of 31 cancer registration areas in Guangxi in 2016 were collected. Cancer incidence and mortality stratified by area（urban/rural），gender and age were analyzed. Crude rate，age-standardized rate（ASR），cumulative rate（0-74 years） and truncated rate were calculated. The proportions and rates of the top 10 cancers in different groups were also calculated. Age-standardized incidence and mortality rates were calculated using the Chinese population census in 2000 and Segi's population. Results The 31 cancer registration areas covered a total of 11，628，368 people（6，698，487 in urban areas and 4，929，881 in rural areas）. Among these people，28，140 new cancer cases were found，and the crude incidence rate，the ASR China，the ASR world and cumulative rate（0-74 years） were 241.99/105， 196.89/105，192.26/105，and 21.90%，respectively. For either the ASR China or the ASR world，the incidence in male was higher than that in female，and the incidence in urban areas was higher than that in rural areas. Cancer incidence increased rapidly after 40 years old and reached the peak at the age group of 80 years old. The top ten cancers of incidence were liver，lung，breast，colorectum，cervix，stomach，nasopharynx，prostate，uterus and brain cancers，which accounted for about 75.65% of all new cancer cases. New cancer death cases amounted to 17，647，and the crude mortality rate，the ASR China，the ASR world and cumulative rate（0-74 years） were 151.76/105，119.05/105，117.31/105，and 13.27%，respectively. For either the ASR China or the ASR world，the mortality in male was higher than that in female，and the incidence in urban areas was lower than that in rural areas. Cancer mortality increased rapidly after 40 years old and reached the peak at the age group of 85 years old. The top ten cancers of mortality included liver，lung，colorectum，stomach，breast，nasopharynx，cervix，esophagus，prostate，and leukemia，accounting for about 81.74% of all cancer death cases. Conclusions The incidence and mortality in Guangxi cancer registration areas in 2016 were slightly higher than 2015. Liver，lung，colorectum，breast，and nasopharynx cancers should be the focus of prevention and treatment. Related Articles | Metrics

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Trend analysis and prediction of lung cancer mortality and incidence in urban areas of Guangzhou LUN Wenhui, LI Tiegang, MAO Chun, LYU Jiachun 2020, 12 (1):  51-56.  doi: 10.3969/j.issn.1674-5671.2020.01.09 Abstract ( 440 )   PDF (34233KB) ( 108 )   Save Objective To analyze the trend of lung cancer incidence and mortality among urban areas in Guangzhou and predict the mortality of lung cancer from 2016 to 2025 in order to provide baseline data for lung cancer prevention strategies. Methods The Joinpoint model was used to analyze the trend of lung cancer incidence from 2002 to 2015 and the trend of lung cancer mortality from 1972 to 2015 in Guangzhou urban areas by calculating the annual percentage change（APC）. The ARIMA model was used to predict the mortality of lung cancer in Guangzhou urban areas from 2016 to 2025. Results From 1972 to 2015，the crude mortality of lung cancer increased from 13.65/105 to 56.11/105（APC=3.3%，P<0.001）. It was predicted that the lung cancer mortality would continue to increase year by year in the future，and reach 65.78/105 in 2025，with an average annual growth rate of 1.60%. The crude incidence rate of lung cancer increased during the period of 2002 to 2015 from 51.84/105 to 68.65/105（APC=2.3%，P<0.05）. Conclusions The mortality of lung cancer in urban areas of Guangzhou will continue to increase year by year in the next 10 years，and the incidence of lung cancer has increased in the past 13 years on the whole，and lung cancer still poses a threat to the health of urban residents in Guangzhou. Related Articles | Metrics

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Expression and clinical significance of mitochondrial calcium uptake 1 in renal clear cell carcinoma tissues#br# HE Yuanfang, CHEN Yanqin, YANG Tao, ZHANG Shaohui, LIU Xiaobin 2020, 12 (1):  57-62.  doi: 10.3969/j.issn.1674-5671.2020.01.10 Abstract ( 272 )   PDF (33331KB) ( 64 )   Save Objective  To investigate the expression and clinical significance of mitochondrial calcium uptake 1（MICU1） in patients with renal clear cell carcinoma（RCCC）. Methods Cases of thirty patients with RCCC，who underwent radical nephrectomy in Yan'an University Affiliated Hospital from January 2016 to December 2018，were investigated. Immunohistochemical method was used to detect the expression of MICU1 protein in RCCC cancer tissues and adjacent normal tissues. Transient transfection was used to transfect the MICU1 interference fragment and the MICU1 overexpression plasmid into CAKI-1 cells（siMICU1 group and MICU1 group），respectively，and corresponding negative controls（siCtrl group and EV group） were set. Western blot was used to detect the expression of MICU1 protein，and MTS and EdU assays were used to detect the proliferation of CAKI-1 cells. The transcriptome data of 517 RCCC tissues in TCGA database were collected，and the relationship between MICU1 mRNA expression and clinicopathological characteristics and prognosis of RCCC patients were analyzed. Results The expression of MICU1 protein in RCCC tissues was lower than that in normal adjacent tissues（P<0.001）. The overexpressed MICU1 cells and interfered MICU1 cells were successfully constructed. MTS and EdU assays showed that the cell proliferation ability of the MICU1 group was lower than that of the EV group（P<0.05），and that of the siMICU1 group was higher than that of the siCtrl group（P<0.05）. TCGA database analysis showed that MICU1 expression was related to gender and TNM stage（P<0.05）； the survival rate of patients with low expression of MICU1 was lower than that of patients with high expression（χ2=19.290，P<0.001）； multivariate Cox regression showed that low expression of MICU1 was an independent risk factor affecting the overall survival of RCCC patients（HR=1.641，95%CI：1.191-2.261，P=0.002）. Conclusion MICU1 has a low expression in RCCC tissues and can promote the proliferation of RCCC cancer cells.  Related Articles | Metrics

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Development and evaluation of a predictive model for primary liver cancer recurrence after radiofrequency ablation CHEN Zhiwei, WU Feixiang, CHEN Jie, WANG Xiaobo, TANG Zhihong, MENG Weida, WEI Meng, ZHANG Lianda, MAI Rongyun, WEI Tao, SU Hengfeng, LI Lequn, BAI Tao 2020, 12 (1):  63-69.  doi: 10.3969/j.issn.1674-5671.2020.01.11 Abstract ( 411 )   PDF (13092KB) ( 186 )   Save Objective To develop and evaluate a nomogram model for predicting the disease-free survival rate of primary liver cancer（PLC） patients after radiofrequency ablation（RFA）. Methods The clinical data of 213 PLC patients receiving RFA in Guangxi Medical University Cancer Hospital from June 2009 to May 2017 were analyzed retrospectively. The PLC patients were randomly divided into a training group（n=133） and a validation group（n=80）. The Cox regression model was used to analyze the factors of recurrence after RFA，and a nomogram model was developed. The model agreement was evaluated by the calibration curve，the applicability of the model was validated by the Kaplan-Meier curve，and the accuracy of the model prediction was evaluated by the C-index. Results The 1-year，3-year，and 5-year disease-free survival rates in the training group were 65.25%，40.91%，and 26.99%，respectively，and those in the validation group were 66.29%，48.10%，and 24.59%，respectively. No significant difference in survival curves was found between the two groups（P=0.785）. The Cox regression analysis showed that the key influential factors of disease-free survival rate included the tumor number（HR=1.921，95%CI：1.136-3.251），hepatitis C antibody positive（HR=4.545，95%CI：1.700-12.149），HBV-DNA≥102 IU/mL（HR=1.993，95%CI：1.209-3.284），and serum prealbumin（HR=0.996，95%CI：0.993-0.999）. Based on the tumor number，HBV-DNA，serum prealbumin，and so on，a nomogram model was developed. The C-indexes of the training group and the validation group were 0.649（95%CI：0.588-0.710） and 0.641（95%CI：0.556-0.724），respectively，and the calibration curve fitted well with the predicted calibration curve in the calibration graph. The patients were divided into the high-risk group and low-risk group by the nomogram，the disease-free survival rate in the high-risk group was lower than that in the low-risk group（P<0.05）. Conclusions The nomogram model based on the tumor number，HBV-DNA，and serum prealbumin can predict well the disease-free survival rate of liver cancer patients after RFA，providing guidance for patient adjuvant treatment. Related Articles | Metrics

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Risk factors and prognostic analysis of lymph node metastasis in No.14v group in gastric cancer LIU Jinlu, QIN Xingan, LI Changhua, LUO Yihuan, CHEN Junqiang 2020, 12 (1):  69-74.  doi: 10.3969/j.issn.1674-5671.2020.01.12 Abstract ( 483 )   PDF (1159KB) ( 200 )   Save Objective To investigate the risk factors and to perform prognostic analysis of lymph node metastasis in the No.14v group in gastric cancer. Methods The clinical data of 72 gastric cancer patients who underwent radical gastrectomy with lymph node in the No.14v group dissection were analyzed retrospectively. Logistic regression was used to analyze the factors affecting lymph node metastasis in the No.14v group，Cox regression was used to analyze the relationship between lymph node metastasis in the No.14v group and prognosis. Results The lymph node metastasis rate in the No.14v group was 25.0%（18/72）. Logistic regression analysis showed that Borromann classification Ⅲ-Ⅳ（OR=81.508，95%CI：4.805-1 382.635，P=0.002），distant metastasis（OR=11.494，95%CI：1.085-121.723，P=0.043），and TNM stages Ⅲ-Ⅳ（OR=8.101，95%CI：1.355-48.437，P=0.005） were independent risk factors affecting lymph node metastasis in the No.14v group. The median overall survival of patients with lymph node metastasis in the No.14v group was lower than that in the non-metastatic group（22 months vs 33 months，χ2= 22.737，P<0.001）. Multivariate Cox regression analysis showed that lymph node metastasis in the No.14v group was an independent influencing factor for the overall survival of patients（HR=2.881， 95%CI：1.222-6.793，P=0.016）. Conclusions Patients with lymph node metastasis in the No.14v group have a poor prognosis in gastric cancer，and the independent risk factors for lymph node metastasis in the No.14v group include Borromann classification Ⅲ-Ⅳ，distant metastasis，and TNM stage Ⅲ-Ⅳ. Related Articles | Metrics

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Clinical efficacy of P-Gemox regimen combined intensity-modulated radiation therapy treatment for early-stage extranodal NK/T cell lymphoma QUAN Xiaoying, WU Chunzhi, CHEN Xiaoyan, LEI Lei, YE Bin, ZHANG Zhihui 2020, 12 (1):  75-80.  doi: 10.3969/j.issn.1674-5671.2020.01.13 Abstract ( 726 )   PDF (2788KB) ( 215 )   Save Objective To explore the efficacy of P-Gemox regimen combined with intensity-modulated radiotherapy for early extranodal NK/T-cell lymphoma（ENKTL）. Methods The clinical data of 73 patients with ENKTL treated in Sichuan Cancer Hospital from March 2012 to October 2017 were analyzed retrospectively. According to the treatment plan，the patients were divided into P-Gemox regimen sandwich radiotherapy group（n=38） and P-Gemox sequential radiotherapy group（n=35），the short-term and long-term clinical effects of the two groups of patients were compared. Results The total effective rate（ORR） of 73 ENKTL patients was 95.9%，the 3-year progression-free survival rate（PFS） was 73.5%，and the 3-year overall survival rate（OS） was 81.9%. The ORR of the P-Gemox regimen sandwich radiotherapy group was 97.4%，and the 3-year PFS and OS were 74.2% and 84.1%，respectively. The P-Gemox regimen sequential radiotherapy group was 94.3%，and the 3-year PFS and OS were 72.6% and 79.2%. There was no significant difference in ORR，PFS，and OS between the two groups（P>0.05）. After adjustment for potential confounders，multivariate Cox regression showed that the PFS of the P-Gemox regimen sandwich radiotherapy group was not inferior to the P-Gemox regimen sequential radiotherapy group（HR=0.617，95%CI：0.353-1.081，P=0.091），while the P-Gemox regimen sandwich radiotherapy group had better OS than the patients of the P-Gemox regimen sequential radiotherapy group（HR=0.556，95%CI：0.314-0.982，P=0.043）. The toxic side effects of chemotherapy in the two groups were mainly Ⅰ-Ⅱ. The P-Gemox regimen sandwich radiotherapy group was more prone to Ⅰ-Ⅱ degree transaminase elevation than the P-Gemox regimen sequential radiotherapy group（P<0.05）. But other toxic side effects，such as hematology and gastrointestinal tract，were not significantly different（P>0.05）. Conclusion The short-term efficacy and PFS of P-Gemox regimen sandwich radiotherapy are similar to those of P-Gemox regimen sequential radiotherapy，while the former can improve the OS of patients compared with the latter. Related Articles | Metrics

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Efficacy of different liver-volume assessment indicators in predicting post-hepatectomy hepatic failure  LU Zhan, GONG Wenfeng, ZHONG Jianhong, MA Liang, ZHANG Zhiming, XIANG Bangde, LI Lequn 2020, 12 (1):  80-85.  doi: 10.3969/j.issn.1674-5671.2020.01.14 Abstract ( 289 )   PDF (2609KB) ( 166 )   Save Objective To evaluate the efficacy of the %RLV（remnant liver volume to total liver volume），RLV/SLV（remnant liver volume to standard liver volume），RLV/BW（remnant liver volume to body weight ratio），and SRLV（standard remnant liver volume） in predicting post-hepatectomy liver failure（PHLF） in hepatocellular carcinoma（HCC） patients under hemihepatectomy. Method Totals of 1，446 patients with hepatitis B virus-associated HCC，who underwent hemihepatectomy in Guangxi Medical University Cancer Hospital from September 2013 to August 2016，were collected and divided into a PHLF group and a PHLF-free group according to whether PHLF occurred after hemihepatectomy. The ROC curve was used to analyze the efficacy of %RLV，RLV/BW，RLV/SLV and SRLV in predicting PHLF. The incidence of postoperative complications between the two groups of patients were compared. Result A total of 181 patients were enrolled in the study，including 22 cases in the PHLF group and 159 cases in the PHLF-free group. There was a statistically significant difference in %RLV，RLV/BW，RLV/SLV，and SRLV between the two groups（P＜0.05）. ROC curve analysis showed that the areas under the curves（AUC） of %RLV，RLV/SLV，RLV/BW，and SRLV were 0.77，0.91，0.84，and 0.91，respectively. The effectiveness of SRLV in predicting postoperative PHLF was better than %RLV and RLV/BW（P<0.05）. According to the SRLV cutoff value of 340 mL/m2，patients were divided into SRLV>340 mL/m2 group and SRLV≤340 mL/m2 group. The incidence of severe postoperative complications in patients with SRLV>340 mL/m2 was statistically significant compared with SRLV≤340 mL/m2（P<0.01）. Conclusion SRLV and RLV/SLV are more effective than RLV/BW and %RLV in predicting PHLF in HCC patients after hepatectomy，and the incidence of PHLF and severe complications are higher when SRLV≤340 mL/m2. Related Articles | Metrics

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Expression and clinical significance of lncRNA SH3PXD2A-AS1 in colon cancer tissues LYU Binghua, ZHONG Luyang, HUANG Qiaoqiao, LI Kezhi, HU Bangli, XIE Mingzhi 2020, 12 (1):  86-90.  doi: 10.3969/j.issn.1674-5671.2020.01.15 Abstract ( 273 )   PDF (5706KB) ( 219 )   Save Objective To investigate the expression of long-chain non-coding RNA（lncRNA） SH3PXD2A-AS1 in colon cancer tissues and its clinical significance. Methods The tumor tissues and corresponding normal tissues were collected from 40 cases of surgical resection at Guangxi Medical University Cancer Hospital. RT-PCR was used to detect the expression level of lncRNA SH3PXD2A-AS1. Colon cancer data from the TCGA database were downloaded and used to verify the relationship between the expression level of lncRNA SH3PXD2A-AS1 and the clinicopathological features and prognosis of colon cancer. Results The expression level of lncRNA SH3PXD2A-AS1 in colon cancer tissues was higher than that in normal adjacent tissues（6.53±1.62 vs 4.37±0.96，t=3.445，P=0.002）. The expression level in the M1 state was higher than that in M0 state，and the expression level of stage Ⅲ-Ⅳ was also higher than that of stage Ⅰ-Ⅱ（P<0.05），though it was not related to the overall survival（χ2=0.326，P=0.568）. Results from the TCGA database showed that lncRNA SH3PXD2A-AS1 was highly expressed in colon cancer tissues，though it was not related to the overall survival（P>0.05）. Conclusions lncRNA SH3PXD2A-AS1 is highly expressed in colon cancer tissues and related to distant metastasis of the tumor，which may become an indicator of the degree of tumor progression. Related Articles | Metrics

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Characteristics and diagnostic value of CT images of pulmonary micro-nodules LI Li, LIU Zhou, YANG Qian, WEN Jie, ZHONG Yihong, WANG Yunfei, LI Tianran, QIAN Yinshu, LUO Dehong 2020, 12 (1):  90-95.  doi: 10.3969/j.issn.1674-5671.2020.01.16 Abstract ( 487 )   PDF (3212KB) ( 203 )   Save Objective To analyze the characteristics of CT images of micro-nodules（≤10 mm in diameter） and explore their qualitative diagnostic value. Methods Multi-slice spiral CT images of 102 pulmonary micro-nodules in 87 patients with pulmonary micro-nodules，pathologically confirmed from March 2017 to November 2018，were retrospectively analyzed. Results Among 102 pulmonary micro-nodules，25 was included in the benign group，27 in the precancerous group and 50 in the malignant group. The incidence of nodal density，burr sign，lobular sign，vascular bundle sign，and pleural indentation sign of the three groups were statistically significant（P<0.05）. Vascular bundle signs in the benign and precancerous groups appeared mainly as clinging or passing through without thickening and distorting，while the malignant group showed mainly passing through with thickening and distorting. There was significant difference in vascular thickening and contouring in the three groups（χ2=19.103，P＜0.001）. However，no significant difference was found in the location of nodules，the occurrence of lung-tumor interface，and vacuole sign among the three groups（P＞0.05）. Conclusions CT images of pulmonary micro-nodules have certain characteristics，and possess important value in the diagnosis of pulmonary micro-nodules. Related Articles | Metrics

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Analysis of risk factors of postoperative pulmonary infection in patients with lung cancer WANG Qinghua, CAI Hongjian, YUAN Rongzheng, GUAN Jie, GAO Jixu 2020, 12 (1):  95-99.  doi: 10.3969/j.issn.1674-5671.2020.01.17 Abstract ( 520 )   PDF (630KB) ( 178 )   Save Objective  To explore the risk factors of postoperative pulmonary infection in patients with lung cancer（PPILC）. Methods A total of 851 lung cancer patients who underwent surgical treatment from March 2011 to February 2018 were selected as the research subjects. The risk factors of PPILC were analyzed by multivariate logistic regression analysis after isolation and identification of pathogenic bacteria. Results There were 60 patients with postoperative pulmonary infection，and 86 pathogenic bacteria were isolated，of which Gram-negative bacteria accounted for 60.5%，mainly Pseudomonas aeruginosa and Klebsiella pneumonia; Gram-positive bacteria accounted for 33.7%，mainly Staphylococcus aureus and Staphylococcus epidermidis; fungus accounted for 5.8%. Multivariate logistic regression analysis showed that the influencing factors of PPILC included TNM stage，smoking，age，merged diabetes，chronic obstructive pulmonary disease，invasive operation，operation time，intraoperative bleeding volume，mechanical ventilation time，total amount of postoperative thoracic drainage，postoperative hemoglobin level，postoperative serum albumin level，duration of thoracic drainage，prophylactic use of antibiotics and length of stay（P<0.05）. Conclusions Age，smoking，concomitant diabetes，chronic obstructive pulmonary disease，and TNM staging can affect the occurrence of PPILC. In clinical practice，more attention should be paid to the prevention and treatment of postoperative pulmonary infection in patients with the above risk factors.  Related Articles | Metrics

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Research progress in the treatment of gastric cancer with lung metastasis OU Hailing, WU Liucheng, QIN Haiquan, HUANG Mingwei, QIN Yuzhou 2020, 12 (1):  99-104.  doi: 10.3969/j.issn.1674-5671.2020.01.18 Abstract ( 354 )   PDF (800KB) ( 230 )   Save Related Articles | Metrics

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Research progress of tumor microenvironment in colorectal cancer HE Kui, HE Langsong, LIU Qi, DENG Yang, WANG Bo 2020, 12 (1):  105-109.  doi: 10.3969/j.issn.1674-5671.2020.01.19 Abstract ( 655 )   PDF (621KB) ( 523 )   Save Related Articles | Metrics

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Research progress of advance care planning in patients with advanced cancer WANG Shuo, LYU Liming, LIU Peipei, YANG Xinyu, ZHU Lijing 2020, 12 (1):  110-114.  doi: 10.3969/j.issn.1674-5671.2020.01.20 Abstract ( 321 )   PDF (588KB) ( 346 )   Save Related Articles | Metrics