Table of Content

25 April 2020, Volume 12 Issue 2 Previous Issue    Next Issue

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Low-grade serous ovarian cancer：Chinese expert consensus（2020 Version） Gynecological Oncology Group, the Invasive and Noninvasive Medical Committee of Chinese Medical Doctor 2020, 12 (2):  117-125.  doi: 10.3969/j.issn.1674-5671.2020.02.01 Abstract ( 642 )   PDF (569KB) ( 316 )   Save Related Articles | Metrics

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Advances in the diagnosis and treatment of hydatidiform mole JI Mingliang, ZHAO Jun, XIANG Yang 2020, 12 (2):  126-130.  doi: 10.3969/j.issn.1674-5671.2020.02.02 Abstract ( 674 )   PDF (498KB) ( 366 )   Save Related Articles | Metrics

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Advances in the treatment of high-risk gestational trophoblastic neoplasms CHEN Lili, LYU Weiguo 2020, 12 (2):  131-135.  doi: 10.3969/j.issn.1674-5671.2020.02.03 Abstract ( 317 )   PDF (450KB) ( 200 )   Save Related Articles | Metrics

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Advances in the treatment of low-risk gestational trophoblastic neoplasms XU Junfen, LYU Weiguo 2020, 12 (2):  136-139.  doi: 10.3969/j.issn.1674-5671.2020.02.04 Abstract ( 297 )   PDF (372KB) ( 198 )   Save Related Articles | Metrics

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Advances in targeting and immunotherapy of gestational trophoblastic neoplasms LI Kemin, YIN Rutie 2020, 12 (2):  140-143.  doi: 10.3969/j.issn.1674-5671.2020.02.05 Abstract ( 407 )   PDF (548KB) ( 132 )   Save Related Articles | Metrics

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Toxicity of chemotherapy in gestational trophoblastic neoplasms and its management ZHONG Lan, YIN Rutie 2020, 12 (2):  144-148.  doi: 10.3969/j.issn.1674-5671.2020.02.06 Abstract ( 506 )   PDF (490KB) ( 313 )   PDF（mobile） (490KB) ( 20 )   Save   Related Articles | Metrics

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Advances in fertility-preserving treatment of gestational trophoblastic neoplasms JIANG Shiyang, ZHAO Jun 2020, 12 (2):  149-154.  doi: 10.3969/j.issn.1674-5671.2020.02.07 Abstract ( 306 )   PDF (569KB) ( 179 )   Save Related Articles | Metrics

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Management of massive hemorrhage in uterine focus of gestational trophoblastic neoplasms WANG Maomao, LI Li 2020, 12 (2):  155-158.  doi: 10.3969/j.issn.1674-5671.2020.02.08 Abstract ( 353 )   PDF (456KB) ( 152 )   Save Related Articles | Metrics

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The value of radiotherapy in the treatment of gestational trophoblastic neoplasms GAO Kun, LI Li 2020, 12 (2):  159-162.  doi: 10.3969/j.issn.1674-5671.2020.02.09 Abstract ( 310 )   PDF (399KB) ( 141 )   Save Related Articles | Metrics

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Effect of salinomycin on the proliferation and apoptosis of triple negative breast cancer cell line MDA-MB-231 by target-regulating ALDH HE Duo, WU Bo, ZHAO Jumei 2020, 12 (2):  162-168.  doi: 10.3969/j.issn.1674-5671.2020.02.10 Abstract ( 385 )   PDF (867KB) ( 134 )   Save Objective To investigate the effect of salinomycin（Sal） on the proliferation and apoptosis of triple negative breast cancer cell line MDA-MB-231 and its mechanism. Methods The flow cytometry was used to screen ALDH highly expressed breast cancer cell lines，and MTT method was used to detect the effect of different concentrations of Sal（1-32 μmol/L） on cell proliferation. The flow cytometry was used to detect the effect of Sal on apoptosis and the expression of ALDH on tumor stem cell surface，qPCR and Western blot were used to detect the mRNA and protein expression of apoptosis-related molecules Caspase-3，Bax，Bcl-2 and PARP. The data of breast cancer patients were downloaded from the Cancer Genome Atlas （TCGA） database（n=1 218），and Pearson method was used to analyze the correlation between ALDH and apoptosis-related molecules expression. Results Sal could inhibit the proliferation of MDA-MB-231 cells in a dose-dependent manner at 24 h，48 h，and 72 h（all P<0.001）. Compared with the control group，the apoptosis rates of the 2 μmol/L group，4 μmol/L group and 8 μmol/L group increased after Sal acting on MDA-MB-231 cells for 48 h（all P<0.05），and the mRNA expression of Bcl-2，PARP and Caspase-3 decreased （all P<0.05），while the mRNA expression of Bax increased（all P<0.05）；the protein expression of Bcl-2 and Caspase-3 decreased（all P<0.05），while the protein expression of PARP and Bax increased（all P<0.05）； the expression of ALDH，a surface marker of tumor stem cells decreased（P<0.05）. TCGA database analysis showed that ALDH was positively correlated with the expression of anti-apoptotic molecules Bcl-2 and Caspase-3（r=0.209，P=0.007；r=0.235，P=0.002），whereas negatively correlated with the expressions of Bax and PARP（r=-0.326，P<0.001； r=-0.453，P<0.001）. Conclusion Sal can inhibit the growth of human breast cancer cell line MDA-MB-231，possibly by down-regulating the expression of ALDH，a surface marker of tumor stem cells. Related Articles | Metrics

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Analysis of the composition of immune cells in the bladder tumor microenvironment based on single-cell mass cytometry Analysis of the composition of immune cells in the bladder tumor microenvironment based on single-cell mass cytometry 2020, 12 (2):  169-174.  doi: 10.3969/j.issn.1674-5671.2020.02.11 Abstract ( 516 )   PDF (1286KB) ( 557 )   Save Objective  To investigate the effect of single-cell mass cytometry（CyTOF） analysis on the characteristics of infiltrating immune cell subsets in bladder cancer tissues and paracancerous tissues，providing a new strategy for the study of bladder cancer heterogeneity. Methods The cancer tissues and paracancerous tissues of 4 bladder cancer patients were collected and prepared into single-cell suspensions by enzymatic hydrolysis and mechanical dissociation. CyTOF was used to detect the expression of 26 immune-related markers in tumor-infiltrating immune cells. Results The bladder CD45+ immune cells were divided into 25 subgroups （7 CD4+ T cell clusters，8 CD8+T cell clusters，4 macrophage clusters，2 B cell clusters，1 dendritic cell cluster，and 3 other types immune cell clusters） by cell similarity analysis. The contents of infiltrating immune cell subpopulations in bladder cancer tissues and paracancerous tissues were different. T cells were more abundant in bladder cancer tissues at 76.73%，whereas B cells and macrophages were more abundant in paracancerous tissues，accounting for 15.20% and 23.82%，respectively. The average proportion of CD4+ Treg cells in bladder cancer tissues was higher than that in paracancerous tissues（2.20% vs 0.81%，P=0.016）. Conclusions CyTOF can effectively distinguish the immune cell subsets in the bladder tumor microenvironment and detect tumor heterogeneity，providing a basis for the accurate treatment of bladder cancer. Related Articles | Metrics

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Effect of lncBRM on the growth of breast cancer cells and its mechanism YANG Jingyu, QIU Shuang, CHEN Xin 2020, 12 (2):  175-180.  doi: 10.3969/j.issn.1674-5671.2020.02.12 Abstract ( 292 )   PDF (735KB) ( 149 )   Save Objective To investigate the effect of long non-coding RNA for association with Brahma（lncBRM） on the growth，migration，and invasion of human breast cancer cell lines（MCF-7，MDA-MB-453） and its possible mechanism. Methods The expression of lncBRM in different breast cancer cells（MCF-7，ZR-75-30，BT474，MDA-MB-231，and MDA-MB-453） and normal human breast epithelial cell line MCF 10A was detected by RT-PCR. siRNA was used to knock down the expression of lncBRM in MCF-7，MDA-MB-453 cells，and transfect si-lncBRM plasmid（si-lncBRM group） and blank plasmid（si-Ctrl group），respectively. CCK-8 assay was used to detect cell proliferation，and flow cytometry was used to detect cell apoptosis. The alteration of cell migration and invasion was detected by Transwell test. In addition，the changes in the expression of E-cadherin and N-cadherin were detected by Western blot. Target prediction of lncBRM was performed by miRDB database. Results The expression level of lncBRM in all breast cancer cells was significantly higher than that in normal human breast epithelial cell line MCF 10A（P<0.01）. Compared with the si-Ctrl group，lncBRM knockdown can inhibit the proliferation of MCF-7 and MDA-MB-453 cells（P<0.05），and increase the apoptosis rate（P<0.01），the numbers of cell invasion and migration cells were small（all P<0.01）. Meanwhile，the protein expression of E-cadherin increased，while N-cadherin decreased dramatically（all P<0.01）. The miRDB database predicted that lncBRM could target 68 miRNAs，and lncBRM knockdown  could indeed increase the expressions of 4 miRNAs（miR-4646-5p，miR-204-3p，miR-204-5p，and miR-6832-3p）of the top 5 miRNAs. Conclusion  lncBRM may inhibit the proliferation，invasion，and migration of breast cancer cells and induce their apoptosis by regulating the miRNAs. Related Articles | Metrics

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Effect of NKX6.3 on proliferation,apoptosis and invasion of esophageal cancer cells and its mechanism ZHENG Yinbin, WU Guojun, LUO Hui, WANG Hua, DU Zonghan 2020, 12 (2):  180-185.  doi: 10.3969/j.issn.1674-5671.2020.02.13 Abstract ( 290 )   PDF (711KB) ( 148 )   Save Objective To investigate the effect of NKX6.3 on proliferation，apoptosis，and invasion of esophageal cancer cells and its possible mechanism. Methods Human esophageal cancer EC9706 cells were transfected and divided into NKX6.3 group（transfected with NKX6.3），miR-NC group（transfected with blank plasmid） and blank control group（Control group）. CCK-8 method was used to detect cell proliferation，the flow cytometry was applied to detect intracellular reactive oxygen species，mitochondrial membrane potential and apoptosis of esophageal cancer cells，and Transwell method was applied to detect invasion of esophageal cancer cells. Expression of proliferation-related protein（Ki67），invasion-related proteins（Vimentin，E-cadherin，and N-cadherin） and apoptosis-related proteins（Bcl-2，Bax，and Caspase-3） were detected by Western blot. Results Statistically significant difference（P<0.01） existed among the Control group，miR-NC group and the expression of NKX6.3 in EC9706 cell in NKX6.3 group，cell proliferation capacity，intracellular reactive oxygen species，mitochondrial membrane potential，cell apoptosis and invasion of esophageal cancer cells，as well as in the expression of Ki67，Vimentin，E-cadherin，N-cadherin，Bcl-2，Bax and Caspase-3 proteins. The expression of NKX6.3，intracellular reactive oxygen species，apoptosis rate，and Vimentin，E-cadherin，Bax and Caspase-3 proteins expression in the NKX6.3 group were significantly higher than those in the miR-NC group，while mitochondrial membrane potential，cell proliferation rate，cell invasion number，and the expression of N-cadherin and Bcl-2 proteins were significantly lower（P<0.01）. Conclusion NKX6.3 can inhibit the proliferation and invasion of esophageal cancer cells and promote cell apoptosis，probably through regulating the expression of proliferation-related proteins and the level of reactive oxygen species in esophageal cancer cells. Related Articles | Metrics

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Effect of adiponectin on the growth of insulin resistance endometrial cancer xenografts in nude mice induced by high-fat diet HE Shan, DENG Lu, CAI Zhifu, ZHANG Jieqing, LI Li 2020, 12 (2):  186-190.  doi: 10.3969/j.issn.1674-5671.2020.02.14 Abstract ( 256 )   PDF (500KB) ( 116 )   Save Objective To investigate the effect of adiponectin on the growth of insulin resistance endometrial cancer xenografts in nude mice induced by high-fat diet. Methods Forty nude mice were randomly divided into a high-fat diet group and a normal diet group，with 20 rats in each group，which were fed with high-fat diet（HFD） and normal diet（ND），respectively. The levels of fasting blood glucose（FBG） and fasting serum insulin（FINS） in nude mice after feeding 10 weeks were measured，and the insulin resistance index（HOMA-IR） was calculated，resulting in a model of insulin resistance in nude mice. At the 11th week，endometrial cancer HEC-1B cells were inoculated into nude mice. After the xenografts reached 0.5 cm，10 nude mice were randomly selected from each group，named as HFD+APN group and ND+APN group，respectively，and adiponectin was intraperitoneally injected into nude mice；the remaining nude mice，named HFD group and ND group，respectively，were injected with 0.9% sodium chloride. After 14 weeks，the metabolism of blood glucose and blood lipids in each group was measured. Results The average body weight，body length，FBG，FINS，and HOMA-IR of nude mice in the high-fat diet group after feeding 10 weeks were larger than those in the normal diet group，and the results of glucose tolerance test and insulin tolerance test showed that the blood glucose level was also higher than that in the normal diet group（all P<0.05）. Nude mice formed tumors after inoculation with endometrial cancer HEC-1B cells，and the modeling was successful. The average weight and volume growth rate of xenografts in the HFD+APN group and the ND+APN group were lower than those in the HFD group and the ND group（all P<0.05）. After 14 weeks，the level of FINS，HOMA-IR，TC and TG of the HFD group and the HFD+APN group were all higher than those of the ND group and the ND+APN group（all P<0.01），and the HFD group was significantly higher than the HFD+APN group（P<0.01）. Serum adiponectin level in the HFD+APN group was significantly lower than that in the ND+APN group（P<0.01），and the HFD group was also significantly lower than the ND group（P<0.05）. Conclusion Adiponectin can improve insulin resistance caused by a high-fat diet，inhibiting the growth of endometrial cancer xenografts. Related Articles | Metrics

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Effect of Docetaxel plus matrix metalloproteinase inhibitor SB-3CT on the growth of prostate cancer xenografts QIAN Li, FU Cuili, LI Ximing, HUANG Guihai, LIN Junhao, LI Wei 2020, 12 (2):  191-195.  doi: 10.3969/j.issn.1674-5671.2020.02.15 Abstract ( 400 )   PDF (538KB) ( 116 )   Save Objective To investigate the effects of Docetaxel plus matrix metalloproteinase inhibitor（SB-3CT） on the growth of prostate cancer xenografts and its possible mechanism. Methods The models of PC3 prostate cancer xenografts were developed based on PC-3 cells in nude mice，which were randomly divided into SB-3CT group（injected with SB-3CT solution 25 mg/kg），combination group（SB-3T 25 mg/kg+intravenous Docetaxel 10 mg/kg） and blank control group（injected with equal amount of normal saline ，with 5 nude mice in each group. The tumor volume and mass were measured，and growth curve were plotted. The expression of PDK1 and PFKFB4 were determined using the immunohistochemical staining. Results The combination group， blank control group and SB-3CT group were statistically significant difference in the average tumor volume（F=29.556，P=0.001），the tumor growth in the combination group was significantly slower than that in the blank control group and the SB-3CT group. The immunohistochemical scores showed that the score of PDK1 in the combination group， the blank control group and the SB-3CT group were 2.33±0.47，6.00±0.81 and 4.33±0.48，respectively，with statistically significant differences（F=18.200，P=0.003），and the score in the combination group was lower than that in the SB-3CT group（P=0.017）；the score of PFKFB4 in those three groups were 5.33±0.49，11.33±0.47 and 9.00±1.41，respectively， with statistically significant differences（F=17.643，P=0.003），and the score in the combination group was lower than that in the SB-3CT group（P=0.011）. Conclusion Docetaxel plus matrix metalloproteinase inhibitor SB-3CT can inhibit the growth of prostate cancer xenografts，and the down-regulation of glucose metabolism-related enzymes PDK1 and PFKFB4 is a potential mechanism. Related Articles | Metrics

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Relationship between abnormal nasal microbiota and prognosis in patients with extranodal NK/T-cell lymphoma WANG Liang, WEI Liqiang, YANG Lei, LI Xin, CONG Jia, YANG Jing, WANG Jingwen 2020, 12 (2):  195-200.  doi: 10.3969/j.issn.1674-5671.2020.02.16 Abstract ( 312 )   PDF (457KB) ( 265 )   Save Objective To investigate the relationship between abnormal nasal microbiota and prognosis in patients with extranodal NK/T-cell lymphoma（ENKTCL）. Methods The clinical data and bacteria culture results of nasal swab of 244 newly diagnosed ENKTCL patients were retrospectively analyzed. The patients were divided into a normal microbiota group（n=114） and an abnormal microbiota group（n=130） according to the presence of abnormal microbiota ，and the correlation analysis was conducted among clinical characteristics，treatment effects and prognosis. Results A total of 409 dominant strains were cultured in patients with abnormal microbiota，mainly staphylococcus aureus，streptococcus aureus，staphylococcus epidermis and pseudomonas aeruginosa. The proportion of patients with elevated lactate denhydrogenase，and medium risk or high risk with a prognostic score of PINK≥1 in the abnormal microbiota group were significantly higher than those of the normal microbiota group（P=0.044；P=0.003）. In the end of treatment，the complete response rate and 5-year progression-free survival of patients in the abnormal microbiota group were significantly lower than those in the normal microbiota group（45.5% vs 61.4%，P<0.05；48.4% vs 63.9%，P=0.048）. Subgroup analysis suggested that in the early stage and low-risk PINK patients，the imbalance of flora was significantly correlated with PFS（P=0.022，0.011）. Conclusions Abnormal nasal microbiota is closely related to the prognosis of patients with ENKTCL. Strategies aiming at abnormal nasal microbiotas may enhance the therapeutic effects and prognosis of ENKTCL. Related Articles | Metrics

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Expression of LGR5 and E-cadherin in gastric cancer tissues and its clinical significance TAN Lin, WANG Qian, CHEN Weishun, AI Qiongjia, LIU Minji, HU Hongsai, QU Weiming 2020, 12 (2):  201-205.  doi: 10.3969/j.issn.1674-5671.2020.02.17 Abstract ( 318 )   PDF (631KB) ( 279 )   Save Objective To investigate the expression of leucine-rich repeat-containing G protein-coupled receptor 5（LGR5） and E-cadherin in gastric cancer tissues and its clinical significance. Methods 115 gastric cancer tissues and 20 corresponding adjacent normal tissues were collected from surgical resection at the Affiliated Zhuzhou Hospital of XiangYa School of Medicine CSU between August 2014 and May 2016. Immunohistochemical staining was performed to determine the expression of LGR5 and E-cadherin. The correlation between LGR5 and E-cadherin expression as well as its relationship with clinicopathological characteristics and prognosis of gastric cancer patients were analyzed. Results The positive rates of LGR5 and E-cadherin in gastric cancer tissues were 62.6% and 53.9%，respectively，compared with the adjacent normal tissues，the difference was statistically significant（P<0.05）. The expression of LGR5 and E-cadherin in gastric cancer tissues were closely related to tumor size，TNM stage，depth of invasion，lymph node and distant metastasis（P<0.05）. Spearman rank correlation analysis indicated that LGR5 expression was negatively correlated with E-cadherin expression in gastric cancer tissues（r=-0.318，P=0.001）. The 1-year and 3-year overall survival rates of LGR5 negative patients were higher than that of positive patients（P<0.001）. The 1-year and 3-year overall survival rates of E-cadherin positive patients were also higher than those of negative patients（P<0.001）. Multivariable Cox regression analysis showed that TNM stage Ⅲ-Ⅳ，lymph node metastasis，distant metastasis，LGR5 positive expression，and E-cadherin negative expression were the independent factors for the prognosis of gastric cancer patients（P<0.05）. Conclusion The high expression of LGR5 in gastric cancer tissues and the lack of E-cadherin expression may lead to the development and poor prognosis of gastric cancer. Related Articles | Metrics

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Expression of PRR11 and SKA2 in cervical cancer tissues and its relationship with prognosis ZHAO Zheng, PANG Ziyao, XU Liping, CHEN Yonghua, YANG Ying 2020, 12 (2):  206-211.  doi: 10.3969/j.issn.1674-5671.2020.02.18 Abstract ( 254 )   PDF (535KB) ( 171 )   Save  Objective To investigate the expression of priline-rich 11（PRR11） and spindle and kinetochore associated complex subunit 2（SKA2） in cervical cancer tissues and its relationship with prognosis. Methods The cervical cancer tissues and adjacent normal tissues of 289 patients with cervical cancer treated in Yantai Yuhuangding Hospital affiliated to Qingdao University from January 2012 to December 2015，were collected. The expression of PRR11 and SKA2 proteins in cervical cancer tissues was detected by immunohistochemiscal staining，and their relationship with the clinicopathological characteristics of patients was analyzed. The Spearman method was used to analyze the correlation between PRR11 and SKA2 expression，Kaplan-Meier was used to analyze the overall survival rate，and Cox proportional risk regression model was used to analyze the prognostic factors. Results The positive expression rate of PRR11 and SKA2 in cervical cancer tissues were higher than those in adjacent normal tissues（60.21% vs 16.96%，χ2=114.081，P＜0.001；69.55% vs 37.37%，χ2=60.143，P＜0.001）. The Spearman correlation analysis results showed that PRR11 was positively correlated with SKA2 protein expression（r=0.725， P<0.001）. Both PRR11 and SKA2 protein expression were related to FIGO stage，tissue differentiation degree and lymph node metastasis（P<0.05）. The 3-year OS of patients with PRR11 and SKA2 positive expression were significantly lower than those of patients with negative expression（78.16% vs 94.78%，P=0.002；82.09% vs 90.91%，P=0.036）. FIGO stage Ⅲ-Ⅳ，PRR11 positive expression and SKA2 positive expression were the independent risk factors affecting the prognosis of cervical cancer patients（HR=1.698，95%CI：1.369-2.876，P＜0.001；HR=2.392，95%CI：1.294-3.596，P＜0.001；HR=1.597，95%CI：1.024-2.302，P＜0.001）. Conclusion PRR11 and SKA2 are highly expressed and positively correlated in cervical cancer tissues. They are independent risk factors affecting the prognosis of patients and may become effective indicators for the diagnosis and prognosis assessment of cervical cancer. Related Articles | Metrics

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Analysis of curative effect of 5-HT3 receptor antagonist combined with Dexamethasone in preventing chemotherapy-induced nausea and vomiting CHEN Junming, WANG Han, WANG Hongxue, HUANG Jinxin, TAN Aihua, ZHOU Wenxian, LU Yongkui, XIE Weimin 2020, 12 (2):  211-216.  doi: 10.3969/j.issn.1674-5671.2020.02.19 Abstract ( 477 )   PDF (439KB) ( 177 )   Save  Objective To investigate the efficacy and safety of the first-generation 5-HT3 receptor antagonist（5-HT3RA） Tropisetron （TRO） and the second-generation 5-HT3RA Palonosetron （PAL），respectively，combined with Dexamethasone （DXM） in the prevention of chemotherapy-induced nausea and vomiting. Methods A total of 192 patients with cancer，treated in the Guangxi Medical University Cancer Hospital from January 2015 to December 2018 through highly emetogenic chemotherapy，were investigated and selected into the PAL group and TRO group by a randomized self-crossover method. Among them，the antiemetic scheme of the PAL group was the PAL+DXM，and the TRO group was the TRO+DXM. The incidence of nausea，vomiting and antemetic adverse reactions were compared between the two groups. Results There was no significant difference in the incidence rate of nausea and vomiting between the PAL group and TRO group （all P>0.05） during acute phase（d1）. In the delayed phase（d2-d5） and whole phase（d1-d5），the nausea rate in the PAL group were markedly lower than that in the TRO group （all P<0.05）. In the delayed phase（d3-d4） and whole phase，vomiting rate in the PAL group were also lower than that in the TRO group （all P<0.05）. 57.3% of patients in the TRO group had to use 5-HT3RA repeatedly，which was significantly higher than 39.3% in the PAL group （P<0.05）. The adverse reactions of antiemetic drugs included constipation，abdominal distension，headache，and hiccups，while the incidence of adverse reactions was similar between the two groups（P>0.05）. Conclusions The second-generation of 5-HT3RA PAL is better than the first-generation of 5-HT3RA TRO in the prevention of delayed chemotherapy-induced nausea and vomiting. Related Articles | Metrics

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Expression of long non-coding RNA RAB11B-AS1 in bladder cancer tissues and its relationship with prognosis JIN Song, CANG Yu, YU Yueping 2020, 12 (2):  217-221.  doi: 10.3969/j.issn.1674-5671.2020.02.20 Abstract ( 253 )   PDF (402KB) ( 154 )   Save Objective To investigate the expression of long non-coding RNA RAB11B-AS1（lncRNA RAB11B-AS1） in bladder cancer tissues and its relationship with clinicopathological parameters and prognosis. Methods The bladder cancer tissues and adjacent normal tissues were collected from 83 cases of surgical resection at the Department of Urology，the Second People's Hospital of Yunnan Province between October 2012 and February 2014. The expression of lncRNA RAB11B-AS1 was detected by qRT-PCR，and its relationship with the clinicopathological characteristics of bladder cancer patients was analyzed. Cox regression was used to analyze the relationship between lncRNA RAB11B-AS1 and the prognosis of bladder cancer patients. Results The expression of lncRNA RAB11B-AS1 in bladder cancer tissues was lower than that in adjacent normal tissues（1.17±0.32 vs 2.09±0.74，t=10.396，P<0.001）. The expression of lncRNA RAB11B-AS1 was correlated with TNM stage，histological grade and lymph node metastasis in patients with bladder cancer（P<0.05）. The 5-year overall survival rate of patients with high expression of lncRNA RAB11B-AS1 was higher than those with low expression（62.22% vs 26.32%，χ2=13.420，P<0.001）. Multivariate Cox regression analysis showed that the low expression of lncRNA RAB11B-AS1 was an independent risk factor for the prognosis of bladder cancer patients（HR=1.126，95%CI：1.058-1.200，P<0.001）. Conclusions The expression of lncRNA RAB11B-AS1 is low in bladder cancer tissues，and the patients with low expression have a poor prognosis. Related Articles | Metrics

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Relationship between the systemic immune-inflammation index and the prognosis of pancreatic ductal adenocarcinoma patients after neoadjuvant chemotherapy ZHAO Yang, CHENG Jing 2020, 12 (2):  222-227.  doi: 10.3969/j.issn.1674-5671.2020.02.21 Abstract ( 381 )   PDF (440KB) ( 160 )   Save Objective To investigate the prognostic value of the systemic immune-inflammation index（SII） in pancreatic ductal adenocarcinoma（PDAC） after neoadjuvant chemotherapy. Methods The clinical data of PDAC patients who received pancreatectomy in Panjin Liao Oil Baoshihua Hospital from January 2013 to December 2016 were analyzed retrospectively. SII was detected after neoadjuvant chemotherapy. The ROC curve was used to determine the optimal cutoff value of SII，by which the patients were divided into SII>885 group（n=37） and SII ≤885 group（n=58）. Cox regression was used to evaluate the relationship between SII and postoperative survival of PDAC patients. Results SII was related to tumor size，biliary drainage，and CA19-9 levels before surgery（P<0.05）. The 3-year survival rate of the SII≤885 group was higher than the SII >885 group（43.1% vs 18.9%，P=0.015）. Multivariable Cox regression showed that intraoperative tumor size>3 cm（HR=1.367，95%CI：1.227-2.215，P=0.031），CA19-9>37 IU/mL（HR=1.292，95%CI：1.132-1.931，P=0.011），SII＞885（HR=1.451，95%CI：1.327-2.431，P=0.021） were independent risk factors of postoperative survival of PDAC patients. Conclusion SII＞885 suggests poor prognosis in patients with PDAC after neoadjuvant chemotherapy.   Related Articles | Metrics

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Implication of the SUVmax of 18F-FDG PET/CT images in predicting the therapeutic effects of non-Hodgkin's lymphoma ZHAO Xiaojia, SU Weipeng, LI Xiaohong, QIN Yongde 2020, 12 (2):  227-231.  doi: 10.3969/j.issn.1674-5671.2020.02.22 Abstract ( 369 )   PDF (409KB) ( 370 )   Save Objective To explore the relationship between the maximum standard uptake value（SUVmax） of 18F-FDG PET/CT images and the therapeutic effects in patients with non-Hodgkin's lymphoma（NHL） before treatment. Methods The clinical data of 146 patients diagnosed with NHL by lymph node biopsy on 18F-FDG PET/CT images in our department from July 2017 to March 2019 were analyzed retrospectively. According to the International Working Organization Evaluation Standard for Lymphoma Efficacy（IWC），NHL patients with 6 courses of chemotherapy were divided into effective and ineffective groups to analyze the relationship between SUVmax and clini-copathological characteristics and curative effect. Results The clinical stage，malignancy，lesion size，necrosis，bone marrow infiltration，serum lactae dehydrogenase before treatment，Ki-67 positive rate in NHL patients were related to the SUVmax（P<0.05）. The SUVmax before treatment of the ineffective group was higher than that of the effective group（16.68±6.16 vs 12.16±5.27，t=4.654，P=0.001）. The optimal cut-off value of SUVmax to evaluate the efficacy of NHL patients before treatment was 14.836，the corresponding AUC was 0.835（95%CI：0.751-0.920），and the specificity was 80.0%，sensitivity was 77.4%. SUVmax>14.836 before treatment was an independent risk factor affecting the efficacy of NHL patients（OR=1.688， 95%CI：1.179-2.418，P=0.007）. Conclusion The SUVmax in 18F-FDG PET/CT images can be used to evaluate the efficacy of NHL patients. Related Articles | Metrics

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Research progress of immune imbalance caused by HBV infection and hepatocellular carcinoma MAO Lisha, SONG Qingfeng, MA Tianjun, CUI Ying 2020, 12 (2):  232-236.  doi: 10.3969/j.issn.1674-5671.2020.02.23 Abstract ( 304 )   PDF (342KB) ( 222 )   Save Related Articles | Metrics

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Lung adenocarcinoma misdiagnosed as lymphoma and lymphatic tuberculosis：a case YANG Xun, WAN Zifen, RAO Shanshan, ZHANG Xiangyan, SHAO Songjun 2020, 12 (2):  237-238.  doi: 10.3969/j.issn.1674-5671.2020.02.24 Abstract ( 332 )   PDF (397KB) ( 144 )   Save Related Articles | Metrics