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    25 October 2020, Volume 12 Issue 5 Previous Issue    Next Issue
    Chinese expert consensus on tumor mutational burden testing and clinical application
    Genetic Tumor Markers Collaboration Group, Tumor Biomarker Committee, China Anti-cancer Association, Molecular Pathology Collaboration Group, Tumor Pathology Committee, China Anti-Cancer Association
    2020, 12 (5):  485-494.  doi: 10.3969/j.issn.1674-5671.2020.05.01
    Abstract ( 2434 )   PDF (1103KB) ( 3175 )   Save
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    Healthy China action and accurate health management of cancer
    ZHOU Zechen, YU Hongping, CHEN Dafang
    2020, 12 (5):  495-500.  doi: 10.3969/j.issn.1674-5671.2020.05.02
    Abstract ( 546 )   PDF (1270KB) ( 664 )   Save
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    The position and function of group medicine in the construction of healthy China
    DING Kexin, YU Hongping, CHEN Dafang
    2020, 12 (5):  501-505.  doi: 10.3969/j.issn.1674-5671.2020.05.03
    Abstract ( 425 )   PDF (641KB) ( 209 )   Save
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    Advances in Oncoplastic Breast Surgery
    Louis Chow Wing-cheong
    2020, 12 (5):  506-510.  doi: 10.3969/j.issn.1674-5671.2020.05.04
    Abstract ( 232 )   PDF (1381KB) ( 110 )   Save

    Evidence from six prospective, randomized trials has shown that breast-conserving surgery(BCS) is a safe alternative to mastectomy, and this led to a heightened interest in achieving and balancing local control and cosmetic outcome post-surgery. However, it is also becoming apparent that conventional BCS techniques may not produce cosmetically favorable results for patients who present with ill-defined or poorly situated breast tumors. Other factors that are commonly found in Chinese women, such as small-volume and denser breasts, also contribute to the difficulty in achieving an optimal cosmetic outcome post-surgery, thus necessitating the need for oncoplastic breast surgery techniques to be employed. This article serves as an overview of the recent advances and principles of oncoplastic breast surgery, as well as the use of autologous fat grafts to improve cosmetic results and eliminate remaining smaller deformities post-surgery.

     

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    The discussion on breast reconstruction after breast cancer surgery
    ZHU Liling, CHEN Kai, LI Shunrong
    2020, 12 (5):  527-531.  doi: 10.3969/j.issn.1674-5671.2020.05.08
    Abstract ( 739 )   PDF (6887KB) ( 67 )   Save
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    Clinical application of “人”-shaped glandular tissue oncoplastic technique in breast-conserving surgery for early breast cancer
    ZHANG Hui, DENG Shuting, YUE Yeli, YU Feng, JING Zhouhong, ZENG Junjie, ZHENG Xiaodong, LIU Xiaoyu
    2020, 12 (5):  532-537.  doi: 10.3969/j.issn.1674-5671.2020.05.09
    Abstract ( 229 )   PDF (4382KB) ( 182 )   Save
    Objective To investigate the clinical effect and safety of“人”- shaped glandular tissue flap oncoplastic technique in breast-conserving surgery for early breast cancer. Methods The clinical data of 78 female patients who received breast-conserving surgery using“人”-shaped glandular tissue flap oncoplastic technique from March 2017 to December 2019 were analyzed retrospectively, and its clinical effects and complications were summarized and analyzed. Results The duration of operation was 65-175 min, with an average time of 98 min; the volume of tissue resected tissue was 47-214 mm3, with an average of 124.3 mm3; the incisal margins of 2 patients showed positive results in the initial cryophistopathological examination, but the incisal margins were confirmed to be negative in intraoperative cryophistopathological examination and postoperative paraffin pathological examination after next resection; 2 patients were removed the nipple-areola complex because the tumor was located in the nipple and areola areas, and incisal margins were positive. In the evaluation of patients' satisfaction with breast cosmetic effect 3 months after operation showed that 71 patients (91.0%) were very satisfied, 6 patients(7.7%) were satisfied, 1 patient(1.3%) was general and no dissatisfied patients were found, with an overall satisfaction rate of 98.7%. In the objective evaluation by specialists with breast cosmetic effect 3 months after operation showed that there were 69 excellent cases(88.5%), 8 good cases(10.3%), and 1 general case (1.3%). Postoperative complications occurred in a total of 3 patients, including 1 case of  postoperative incision infection, 1 case of local subcutaneous, and 1 case of partial nipple and areola ischemia, the complication rate was 3.8%, and all recovered after symptomatic treatment without delay of postoperative adjuvant treatment. Conclusions The breast-conserving surgery using “人”-shaped  glandular tissue flap oncoplastic technique has a low incidence of postoperative complications and a satisfactory cosmetic effect.
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    Effect of REST4 on biological behavior of glioma U87 cells and its mechanism
    LI Huashun, NIU Dongsheng, CAO Shaoxiang
    2020, 12 (5):  538-542.  doi: 10.3969/j.issn.1674-5671.2020.05.10
    Abstract ( 338 )   PDF (7132KB) ( 59 )   Save
    Objective To investigate the effect of repressor element 1-silencing transcription factor 4 (REST4) gene on the proliferation, apoptosis and migration of glioma U87 cells and its possible mechanism. Methods REST4-siRNA was transfected into glioma U87 cells (si-REST4 group), while siRNA negative control group (si-NC group) and Control group were set up. The expression of REST4 was detected by the real-time quantitative PCR, the ability of cell proliferation was detected by MTT method, the cell apoptosis rate was detected by Annexin V-FITC/PI double staining, and the ability of cell migration was detected by scratch test. The expressions of REST4, PI3K, AKT, mTOR, p-PI3K, p-AKT and p-mTOR proteins in U87 cells were detected by Western blot. Results Compared with the Control group and si-NC group, the REST4 mRNA and protein levels of U87 cells in the si-REST4 group were down-regulated (P<0.05), the ability of cell proliferation decreased (P<0.05), the proportion of cell apoptosis increased(P<0.05), and the cell migration decreased (P<0.05). Western blot showed that compared with the Control group and the si-NC group, the protein expression levels of PI3K, AKT, mTOR, p-PI3K, p-AKT and p-mTOR of U87 cells in the si-REST4 group decreased (P<0.05). Conclusions REST4 may inhibit the proliferation and migration of glioma U87 cells and promote their apoptosis through PI3K/AKT/mTOR signal pathway.
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     Effect of chidamide on proliferation of rituximab-resistant B-cell lymphoma cells and its potential mechanism
    KE Qing, LUO Zhao, CEN Hong
    2020, 12 (5):  543-547.  doi: 10.3969/j.issn.1674-5671.2020.05.11
    Abstract ( 307 )   PDF (10006KB) ( 53 )   Save
    Objective To investigate the effect of histone deacetylase(HDAC)  inhibitor chidamide on the proliferation of rituximab-resistant B-cell lymphoma cells in vitro and its potential mechanism. Methods The proliferation inhibition rate of lymphoma Jeko-1 and Jeko-1/R cells was detected by CCK-8 method after treated with different concentrations of chidamide, rituximab or combination drugs. The expression of CD20 mRNA was detected by the RT-PCR, and the acetylation levels of histone H3 and H4 was detected by Western blot. Results 25 μg/mL, 50 μg/mL, 100 μg/mL and 200 μg/mL of rituximab could inhibit the proliferation of Jeko-1 cells in a dose-dependent manner at 48 h and the difference between the groups was statistically significant(F=38.533, P<0.001), and the proliferation inhibition rate was higher than that of Jeko-1/R cells (all P<0.001). 4 μmol/L, 8 μmol/L and 16 μmol/L of chidamide could inhibit the proliferation of Jeko-1 and Jeko-1/R cells in a dose-dependent manner at 48 h and the difference between the groups was statistically significant(F=17.968, P=0.003; F=51.456, P<0.001), whereas there was no statistically significant difference in the proliferation inhibition rate of Jeko-1 and Jeko-1/R cells exposed to chidamide with different concentrations (all P>0.05). When Jeko-1 cells and Jeko-1/R cells were treated by combining chidamide(8 μmol/L) with rituximab(100 μg/mL) for 48 h, their proliferation inhibition rates were higher than those treated by single-agent chidamide(P<0.001). Chidamide could up-regulate CD20 mRNA expression, histone H3 and H4 acetylation levels of Jeko-1/R cells in a dose-dependent manner at 48 h(P<0.001). Conclusion Chidamide can inhibit the proliferation of drug-resistant B-cell lymphoma Jeko-1/R cells, potentially up-regulating histone H3 and H4 acetylation levels and CD20 mRNA expression.
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    Influence of miR-572 on the malignant biological behavior of lung cancer cells via targeting WWOX regulation
    YU Yuanyuan, LEI Huaiding, WU Chenglin, ZHAO Su, WANG Meifang
    2020, 12 (5):  548-554.  doi: 10.3969/j.issn.1674-5671.2020.05.12
    Abstract ( 243 )   PDF (32182KB) ( 42 )   Save
    Objective To investigate the molecular mechanism of miR-572 targeting WW domain containing oxidoreductase(WWOX) in regulating the proliferation and apoptosis of lung cancer cells. Methods A total of 50 cases of lung cancer tissues and their corre-sponding adjacent tissues of lung cancer patients who underwent surgical resection in Taihe Hospital of Shiyan Gity from March 2016 to May 2018 were collected. miR-572 inhibitor and miR-572 mimics were transfected into lung cancer A549 and L9981 cells by using liposome transfection technology. The expressions of miR-572 and WWOX mRNA were determined by qRT-PCR, the cell proliferation was measured by MTT assay, and the cell cycle and apoptosis were measured by flow cytometry. Targetscan was used to analyze the target genes of miR-572, the luciferase reporter gene experiment confirmed the targeting relationship between miR-572 and WWOX. Results miR-572 was highly expressed in lung cancer tissues and cells ( all P<0.05). After down-regulating miR-572, the proliferation ability of lung cancer A549 and L9981 cells decreased(all P<0.05), but the cell G0/G1 phase ratio and apoptosis rate increased(all P<0.05). However, after up-regulating miR-572, the proliferation ability of miR-572 in lung cancer A549 and L9981 cells increased (all P<0.05), while the cell G0/G1 phase ratio and apoptosis rate of cells decreased(all P<0.05). WWOX was low expressed in lung cancer tissues and cells( all P<0.05), and it was negatively correlated with the expression of miR-572 in lung cancer tissues(r=-0.669, P<0.001). Down-regulating the expression of WWOX could reverse the effect of miR-572 on lung cancer cell proliferation inhibition, cycle arrest and apoptosis promotion. Conclusion Down-regulation of miR-572 can inhibit the proliferation and induce apoptosis of lung cancer cells, probably through targeting negative regulation of WWOX.
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    The value of combined detection of miR-21、miR-204 and miR-34a in diagnosis of gastric cancer
    吴海玲, 袁冲
    2020, 12 (5):  555-559.  doi: 10.3969/j.issn.1674-5671.2020.05.13
    Abstract ( 338 )   PDF (6007KB) ( 131 )   Save
    Objective To investigate the expression levels and the clinical diagnostic value of miR-21, miR-204 and miR-34a in serum of gastric cancer patients. Methods A total of 42 patients with gastric cancer, 22 patients with benign gastric lesion and 25 healthy volunteers, who were treated at the First Affiliated Hospital of Air Force Medical University from February 2017 to January 2018, were collected. The expressions of serum miR-21, miR-204 and miR-34a were assessed by the real-time PCR, and the diagnostic efficiency were evaluated by the receiver operating characteristic(ROC) curve. Results The expression of miR-21 in the serum of gastric cancer patients was higher than that of benign lesion and healthy volunteers(all P<0.001), but the expressions of miR-204 and miR-34a were lower than those of the other two groups(all P<0.001). The expression of miR-204 was correlated with distant metastasis(P=0.048). ROC curve showed that the areas under the curve(AUC) of miR-21, miR-204 and miR-34a in the single diagnosis of gastric cancer were 0.736, 0.724 and 0.654, respectively; the AUC of combined diagnosis was 0.849, which was significantly higher than that of single diagnosis(all P<0.05), and the sensitivity and specificity were 92.86% and 65.96%, respectively. The AUC of combined diagnosis of early stage gastric cancer patient was 0.828, and the sensitivity and specificity were 87.23% and 69.23%, respectively. Conclusion Combined detection of serum miR-21, miR-204 and miR-34a can improve diagnostic efficiency of gastric cancer, with important value in the diagnosis of early gastric cancer.
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    Development of a prognostic nomogram and risk stratification system for cervical carcinoma after operation
    LUO Shanhui, ZHU Weipei
    2020, 12 (5):  560-565.  doi: 10.3969/j.issn.1674-5671.2020.05.14
    Abstract ( 487 )   PDF (14995KB) ( 155 )   Save
    Objective To construct a nomogram predictive model for postoperative cervical cancer patients, and establish a risk strat-ification system based on the individual scores of the nomogram. Methods The data of 6, 835 postoperative cervical cancer patients in the US SEER (Surveillance, Epidemiology, and End Results) database from 1973 to 2015 was collected to construct a predictive model, and 120 patients who underwent cervical cancer surgery at the Second Affiliated Hospital of Soochow University were selected as an external validation cohort. Through univariable and multivarible Cox regression analysis, prognostic factors were screened out to draw a nomogram, and a risk stratification system was established based on the prognostic risk score of the nomogram. Results The Cox regression analysis showed that the age at diagnosis, race, histological grade, T stage, N stage, lymph node dissection status, tumor size and depth of tumor invasion were independent of prognostic factors for patients with cervical cancer after surgery. The C-index of nomogram constructed in the training cohort, internal verification cohort and external cohort were 0.824, 0.814, and 0.730, respectively. The calibration curve showed that the model prediction was basically consistent with the actual survival situation, and the risk stratification system could clearly distinguish the survivals of patients with different FIGO stages (all P<0.05). Conclusions The nomogram can effectively predict the postoperative prognosis of patients with cervical cancer after surgery. The risk stratification system based on the nomogram prediction model has clinical value in distinguishing high-risk patients.
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    A bioinformatics-based analysis of immune resistance modes mediated by high frequency mutation of STIM1 in colorectal cancer and related molecular mechanisms
    YUAN Hao, ZHONG Huage, YAN Linhai, TANG Weizhong
    2020, 12 (5):  566-571.  doi: 10.3969/j.issn.1674-5671.2020.05.15
    Abstract ( 318 )   PDF (16194KB) ( 104 )   Save
    Objective To investigate the gene mutation spectrum of colorectal cancer (CRC) and the mediated molecular mechanism of CRC immune resistance. Methods A total of 36 CRC patients admitted to Guangxi Medical University Cancer Hospital from January 2019 to January 2020 were selected. New generation sequencing(NGS) was used to detect the gene mutation spectra in tissue and blood samples. The mutation spectra were described by molecular network events, TIMER and CancerSEA database were used to analyze the immune infiltration and tissue single cell distribution of core molecules, Reactome database was used for functional clustering analysis of core molecules, and the STRING database was used to analyze the core immune resistance event network. Results The results of tissue and blood samples showed that stromal interaction molecule 1(STIM1) was the screened high frequency mutation gene (The mutation frequency was 97.2%). Immune infiltration and single cell distribution showed that STIM1, RELA and JUN were involved in immune response. The expressions of STIM1, RELA and JUN mRNA in colon cancer patients were significantly correlated with the infiltration level of CD4+ T cells(r=0.554, 0.565, 0.319; all P<0.05). Meanwhile, RELA and JUN were positively correlated with STIM1(r=0.579, 0.223;all P<0.05). Kaplan-Meier analysis indicated that the higher the infiltration levels of CD4+ T cells and macrophages are, the worse the survival prognosis of colorectal cancer patients is. Functional cluster analysis identified that STIM1 was related to adaptive immune system, innate immune system, cytokine signaling in the immune system and ubiquitination modification of immunoreactive proteins, while PPI network analysis showed that STIM1 was related to RELA and JUN. Conclusions STIM1 may join RELA and JUN to form an immune resistance network, and becomes an important regulatory factor and potential therapeutic target in CRC immune resistance.
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    Value of the aspartate aminotransferase to lymphocyte ratio in evaluating prognosis of primary liver cancer with portal vein tumor thrombus patients before TACE
    TANG Zhihong, YUAN Du, CHEN Zhiwei, MENG Weida, WU Feixiang, WANG Xiaobo
    2020, 12 (5):  572-577.  doi: 10.3969/j.issn.1674-5671.2020.05.16
    Abstract ( 358 )   PDF (2231KB) ( 202 )   Save
    Objective To investigate the value of the aspartate aminotransferase to lymphocyte ratio (ALRI) in the prognosis evaluation of primary liver cancer(PLC) with portal vein tumor thrombosis(PVTT) patients before transcatheter arterial chemoembolization (TACE). Methods A total of 175 patients with PLC and PVTT who received TACE treatment in Guangxi Medical University Cancer Hospital from June 2013 to June 2018 were selected. The optimal critical value of ALRI was determined by the time-dependent ROC curve, the independent predictors of overall survival(OS) were analyzed by Cox regression model, and the survival rate was calculated by Kaplan-Meier method. Results The ROC curve showed that the optimal clinical value of ALRI was 49.37, and the corresponding AUC was 0.71. Kaplan Meier analysis showed that the OS of patients with ALRI>49.37 was shorter than that of patients with ALRI≤49.37(P=0.003). Cox regression analysis showed that ALRI>49.37, more than one TACE treatment, Child-Pugh grade B, and prothrombin time≥13 s were among the independent risk factors for OS in PVTT patients after TACE (all P<0.05). Conclusion ALRI>49.37 before TACE is an independent risk factor for OS in patients with PLC-PVTT.
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    Research progress of XPO1 inhibitors in hematological malignancies
    ZHANG Jiahui, WANG Liang
    2020, 12 (5):  578-583.  doi: 10.3969/j.issn.1674-5671.2020.05.17
    Abstract ( 408 )   PDF (769KB) ( 234 )   Save
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    Research progress of PARP inhibitors in uterine malignant tumors
    ZHANG Heng, KONG Weimin
    2020, 12 (5):  584-587.  doi: 10.3969/j.issn.1674-5671.2020.05.18
    Abstract ( 359 )   PDF (638KB) ( 142 )   Save
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    Research progress of NLRP1 inflammasome and tumorigenesis
    HUANG Fengze, LIANG Wanwang, SUN Daxin
    2020, 12 (5):  594-598.  doi: 10.3969/j.issn.1674-5671.2020.05.20
    Abstract ( 458 )   Save
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