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25 February 2023, Volume 15 Issue 1 Previous Issue    Next Issue

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Chinese expert consensus on the clinical applications of anti⁃angiogenic small molecule tyrosine kinase inhibitors in recurrent metastatic or advanced gynecologic tumors (2022 Edition) Gynecological Oncology Expert Committee of Chinese Society of Clinical Oncology Basic Research 2023, 15 (1):  1-10.  doi: 10.3969/j.issn.1674-5671.2023.01.01 Abstract ( 193 )   PDF   Save Related Articles | Metrics

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Overexpressing miR⁃486⁃5p enhanced radiosensitivity of radioresistant nasopharyngeal carcinoma cell line CNE⁃2R SONG Yangguang, SUN Yongchu, QIN Yuelan, CHEN Kaihua, LAI Lin, CHEN Xishan, ZHU Xiaodong 2023, 15 (1):  11-17.  doi: 10.3969/j.issn.1674?5671.2023.01.02 Abstract ( 104 )   PDF   Save Objective To investigate the radiosensitivity effect of radioresistant nasopharyngeal carcinoma cell line CNE⁃2R after overexpressing miR⁃486⁃5p. Methods microRNA(miRNA) sequencing and RT⁃qPCR were used to detect the expression level of miR⁃486⁃5p in the parent cell line CNE2 and the radioresistant cell line CNE⁃2R. CNE⁃2R cells were infected with miR⁃486⁃5p overexpress⁃ion and negative control lentivirus, which were defined as CNE⁃2R⁃Mimics group and CNE⁃2R⁃NC group, respectively. CCK⁃8 assay and Clonogenic assay were used to detect the proliferation ability and the viability of cells irradiated with different dose of (0 Gy, 2 Gy, 4 Gy, 6 Gy, 8 Gy) X⁃ray. The cell apoptosis was detected by Annexin V⁃APC/7⁃AAD double stain assay. Results miRNA sequencing and RT⁃qPCR results showed that the level of miR⁃486⁃5p in cell line CNE⁃2R was significantly lower than that in parent cell line CNE2. CCK⁃8 assay revealed that the cell proliferation of CNE⁃2R cells overexpressing miR⁃486⁃5p in CNE⁃2R⁃Mimics group was decreased compared with that in the CNE⁃2R⁃NC group after 48 h, 72 h and 96 h culture (all P<0.05). After the cells were irradiated with 2 Gy, 4 Gy, 6 Gy and 8 Gy irradiation, the survival fraction of CNE⁃2R⁃Mimics group was lower than that of the CNE⁃2R⁃NC group (all P<0.05) , with the largest difference in the 8 Gy dose (t=30.152, P<0.001). Flow cytometry analysis indicated that the apoptosis rate of the CNE⁃2R⁃Mimics group was higher than that of  CNE⁃2R⁃NC group at the irradiation dose of 0 Gy and 8 Gy (all P<0.05), and the difference of apoptosis rate under the irradiation dose of 8 Gy was more significant (t=14.945, P<0.001). The Clonogenic assay revealed that the clone formation fraction of the CNE⁃2R⁃Mimics group was lower than that of the CNE⁃2R⁃NC group under different doses, in which the radiobiological parameters D0, Dq, and the survival fraction in irradiation of 2 Gy in CNE⁃2R⁃Mimics group were lower than that of the CNE⁃2R⁃NC group (all P<0.05). Conclusions The nasopharyngeal carcinoma radioresistant cell line CNE⁃2R overexpressing miR⁃486⁃5p may increase radiosensitivity by inhibiting cell proliferation and promoting apoptosis. Related Articles | Metrics

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Effects of pristimerin on proliferation and apoptosis of bladder cancer cells and its mechanism ZHOU Xin, YANG Jin, CHEN Lin, DAI Wenbin, CHEN Bo, LONG Xiaoming, WANG Weiwei, CHENG Qisen, DONG Taotao, CHEN Shulian, LIANG Guobiao 2023, 15 (1):  18-24.  doi: 10.3969/j.issn.1674?5671.2023.01.03 Abstract ( 102 )   PDF   Save Objective To investigate the effects of pristimerin on the proliferation and apoptosis of human bladder cancer HT⁃1376 cells and the related mechanisms. Methods The target protein of pristimerin was predicted by TCMSP, SwissTargetPrediction database and PharmMapper database, and the target of bladder cancer was obtained from GeneCards database. The potential targets of pristimerin in the treatment of bladder cancer were integrated through the Venny 2.1.0 platform; the String database and Cytoscape 3.9.1 software were used to construct a protein⁃protein interaction (PPI) network. GO function and KEGG pathway enrichment analyses were performed by DAVID database. The normal human bladder epithelial cells SV⁃HUC⁃1 and human bladder cancer cell line HT⁃1376 cells were cultured in vitro, and treated with 0-0.8 μmol/L pristimerin. The effects of pristimerin on the proliferation and apoptosis of HT⁃1376 cells were detected by CCK⁃8 assay, colony formation assay, Hoechst⁃PI staining assay. Western blot was used to detect the expression levels of apoptosis⁃related proteins Bcl⁃2, Bax, Cleaved PARP, PARP, and MEK/ERK signaling pathway⁃related proteins p⁃MEK, p⁃ERK1/2. Results  There were 292 targets of pristimerin, among which 114 potential targets were related to bladder cancer treatment, involving 434 biological processes, 54 cellular components and 114 molecular functions, mainly enriched in 132 signaling pathways such as MAPK signaling pathway, lipid and atherosclerosis, etc. The results of in vitro experimental verification showed that pristimerin could significantly inhibit the viability and colony formation ability of HT⁃1376 cells and induce cell apoptosis(all P<0.05). Western blot showed that compared with untreated control group, the expression levels of apoptosis⁃related proteins Bax and Cleaved PARP were increased, Bcl⁃2 and PARP were decreased, and MEK/ERK signaling pathway⁃related proteins p⁃MEK and p⁃ERK1/2 were decreased after treated with 0.65 μmol/L pristimerin (all P<0.05). Conclusions Pristimerin may inhibit the proliferation and induce apoptosis of bladder cancer cells by regulating the phosphorylation level of MEK/ERK signaling pathway⁃related proteins. Related Articles | Metrics

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Regulation of EBV⁃miR⁃BART17⁃5p on proliferation and migration of nasopharyngeal #br# carcinoma cells and its mechanism#br# HUANG Yujuan , DENG Yayan, LIU Xue , CHEN Yong, LI Qiuyun, YE Jiaxiang, ZHANG Jinyan, LI Yongqiang 2023, 15 (1):  25-30.  doi: 10.3969/j.issn.1674-5671.2023.01.04 Abstract ( 236 )   PDF   Save Objective To investigate the regulation of EBV⁃miR⁃BART17⁃5p encoded by epstein⁃barr virus (EBV) on the proliferation and migration of nasopharyngeal carcinoma cells and its mechanism. Methods The lentivirus  Vector control and EBV⁃miR⁃BART17⁃5p mimic were infected into HK1⁃EBV cells, and recorded as the Vector control group and the Mimic group, respectively. RT⁃qPCR was used to verify the infection efficiency of lentivirus. EdU and CCK⁃8 were used to detect the proliferation ability of cells. Colony formation ability of cells was detected by plate cloning assay. Cell migration ability was detected by cell scratch assay and Transwell assay. The protein expression of EBV⁃miR⁃BART17⁃5p target gene PTEN was detected by Western blot. Results Compared with the Vector control group, the expression of EBV⁃miR⁃BART17⁃5p in HK1⁃EBV cells of Mimic group was significantly increased (P<0.001), and the ability of cell proliferation, colony formation and migration were significantly enhanced (all P<0.05). The expression level of PTEN protein was significantly decreased (P<0.001). Conclusions EBV⁃miR⁃BART17⁃5p can regulate the proliferation and migration of HK1⁃EBV nasopharyngeal carcinoma cells, and the mechanism may be related to the reverse regulation of the expression of tumor suppressor PTEN by EBV⁃miR⁃BART17⁃5p. Related Articles | Metrics

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SIRT4 inhibits the proliferation and invasion of pancreatic cancer cells and the growth of transplanted tumor in nude mice by regulating MAPK signaling pathway and lipid metabolism pathway LIU Jun, CHEN Haiqiang, LIU Shiqian, TANG Bo 2023, 15 (1):  30-37.  doi: 10.3969/j.issn.1674-5671.2023.01.04 Abstract ( 131 )   PDF   Save Objective To investigate the effect of SIRT4 on the proliferation and invasion of pancreatic cancer cells and the growth of transplanted tumor in nude mice by regulating MAPK signaling pathway and lipid metabolism pathway. Methods The cancer tissues and adjacent tissues samples of 5 patients with pancreatic ductal adenocarcinoma, who underwent surgical treatment in the Department of Hepatobiliary Surgery of the First Affiliated Hospital of Guangxi Medical University from June 1, 2021 to July 28, 2022, were collected. The Human normal pancreatic cell lines (HPDE6⁃C7) and pancreatic cancer cell lines (CFPAC⁃1, PANC⁃1, BXPC⁃3, ASPC⁃1) were selected, and the expression level of SIRT4 in pancreatic cancer tissues and cells were detected by RT⁃qPCR and Western blot. BXPC⁃3 and PANC⁃1 cell lines overexpressing SIRT4 were constructed by plasmid transfection, and cell proliferation was detected by CCK⁃8 assay and plate clone formation assay. Transwell assay was used to detect cell invasion ability. The BXPC⁃3 cell line stably overexpressing SIRT4 was constructed by lentiviral vector and inoculated into the armpits of nude mice, the effect of SIRT4 on tumor growth in vivo was observed by tumor formation experiment in nude mice. Immunohistochemistry was used to detect the expression of Ki67 in the transplanted tumor of nude mice. The expression of SIRT4 downstream molecules was detected by mRNA⁃seq, and the expression levels of MAPK signaling pathway and lipid metabolism related proteins were detected by Western blot. Results RT⁃qPCR and Western blot results showed that SIRT4 mRNA and protein expression levels in pancreatic cancer tissues were significantly lower than those in adjacent tissues (all P<0.05). The results of CCK⁃8 assay, colony formation assay and Transwell invasion assay showed that compared with the NC group, the proliferation and invasion ability of BXPC⁃3 and PANC⁃1 cells in the SIRT4⁃OE group were decreased (all P<0.05). The tumor formation experiment in nude mice showed that the volume and weight of transplanted tumors were significantly reduced after SIRT4 overexpression (all P<0.05). Immunohistochemistry showed that the number of Ki67 positive cells was significantly reduced in the SIRT4⁃OE group compared with the NC group (P=0.002). The results of mRNA⁃seq and enrichment analysis showed that the downstream differential genes of SIRT4 were significantly enriched in MAPK signaling pathway Western blot showed that the expression of p⁃ERK protein related to MAPK signaling pathway decreased after SIRT4 overexpression, while the protein expression levels of JNK, p⁃JNK, p38 and p⁃p38 changed insignificantly. The protein expression levels of ACC, FASN and SREBP1C, which were the key factors of lipid production, were decreased, while the protein expression levels of PPARα and CPT1a, which were the regulators of lipid metabolism, were increased. Conclusions SIRT4 may inhibit the proliferation and invasion of pancreatic cancer and the growth of transplanted tumor in nude mice by inhibiting the MAPK signaling pathway and lipid metabolism pathway. Related Articles | Metrics

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Analysis of the incidence trend of malignant tumors of digestive system in Hefei, 2011—2015 WANG Xin, ZHANG Xiaopeng, LI Jiajia, SUN Feng, YANG Linsheng, ZHA Zhenqiu 2023, 15 (1):  38-44.  doi: 10.3969/j.issn.1674-5671.2023.01.06 Abstract ( 177 )   PDF   Save Objective To analyze the incidence characteristics and changing trends of malignant tumors of digestive system in Hefei from 2011 to 2015. Methods The incidence data of malignant tumors of digestive system in Hefei from 2011 to 2015 were collected, and the crude incidence rate (CIR), age⁃standardized incidence rate by Chinese standard population (ASIRC), and age⁃standardized incidence rate by Segi's world population (ASIRW) were calculated. The annual percent change (APC) of the ASIRC was calculated by using Joinpoint software to evaluate the trend of the incidence of malignant tumors in the digestive system over time, and the APC and average annual percent change (AAPC) of crude incidence were also calculated to evaluate the trends of age of digestive malignant tumors incidence with respect to gender and urban⁃rural areas. Results From 2011 to 2015, the number of digestive system malignancies in Hefei was 53, 990 cases, with CIR of 162.29/105, ASIRC of 135.57/105 and ASIRW of 135.93/105, accounting for 55.66% of the total number of new malignant cases. The overall incidence gradually increased during 5 years, but the trend was not significant (APC=2.36%, 95%CI: -7.75%-13.58%, P=0.527). The top 5 tumors in incidence were gastric cancer, esophageal cancer, colorectal cancer, liver cancer, and pancreatic cancer, accounting for 53.64% of the total number of new malignant cases, among which the incidence of colorectal cancer continued to rise over time(APC=9.82%, 95%CI: 0.86%-19.57%, P=0.039), while the incidence of other cancers were increased, though insignificantly (all P>0.05). The overall incidence increased gradually with age over the 5⁃year period and rapidly after the age of 30 years old. The incidence rates of age⁃specific in different genders and urban/rural areas were consistent, and the incidence increased rapidly in the age group of ≥25 years old (APC=9.60%, 95%CI: 8.01%-11.20%, P<0.001) and the age group of ≥20 years old (APC=10.74%, 95%CI: 9.30%-12.21%, P<0.001), respectively, among which the incidence in the age group of ≥40 years old were higher in males than in females. The incidence of malignant tumor of digestive system, gastric cancer and esophageal cancer were higher in rural areas than in urban areas in the age group of ≥20 years old, while the incidence of colorectal cancer and pancreatic cancer were higher in urban areas than in rural areas in the age group of ≥ 50 years old. Conclusions  From 2011 to 2015, the incidence of malignant tumors of the digestive system in Hefei increased gradually, but the overall trend of change was relatively stable, showing that male were higher than female and rural areas were higher than urban areas. The incidence trends of age⁃specific in different genders and areas were consistent. In the future, Hefei still needs to strengthen the prevention and treatment of malignant tumors of the digestive system.  Related Articles | Metrics

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Analysis of the incidence and mortality of malignant tumors in the cancer registration areas of Hainan,2017 ZHANG Xu, HUA Jing, FAN Kangqiong, SU Liangping, WANG Dingbin 2023, 15 (1):  45-51.  doi: 10.3969/j.issn.1674-5671.2023.01.07 Abstract ( 203 )   PDF   Save Objective To analyze the incidence and mortality of malignant tumors in the cancer registration areas of Hainan province in 2017. Methods The incidence and mortality of malignant tumors and population data in 6 cancer registries in Hainan province in 2017 were collected, and stratified by sex, region and age after data quality verification. The crude incidence and mortality rate, composition ratio, cumulative rate of 0-74 years old and standardized rate were calculated, and the top 10 malignant tumors in incidence and death were summarized. Results In 2017, the registered tumor population accounted for 23.19% of the total population in Hainan province, and there were 5, 504 new cases of malignant tumors. The incidence, age⁃standardized incidence rate by Chinese standard population (ASIRC), age⁃standardized incidence rate by Segi's world population (ASIRW) and cumulative rate of 0-74 years old were 260.71/105, 213.29/105, 207.88/105 and 24.32%, respectively. There were 2, 585 deaths due to malignant tumors, and the mortality, ASIRC rate, ASIRW rate and cumulative rate of 0-74 years old were 122.44/105, 95.27/105, 94.05/105 and 11.05%, respectively. The incidence and mortality rate of males were higher than those of females, the incidences of both males and females began to increase from the age group of 25- years, reached the peak in the age group of 65- and 80- years, respectirely; The mortality rate increased from 35- years and reached the peak in the age group of 80- years. The incidence rate was higher in urban areas than in rural areas, whereas the mortality rate was lower in urban areas than in rural areas. The top 10 malignant tumors with regard to incidence were liver cancer, lung cancer, colorectal cancer, breast cancer, gastric cancer, nasopharyngeal cancer, esophageal cancer, oral cancer, cervical cancer, and thyroid cancer, while the top 10 malignancies in mortality were liver cancer, lung cancer, gastric cancer, colorectal cancer, nasopharyngeal cancer, esophageal cancer, brain tumor, laryngeal cancer, lymphoma, and breast cancer. Conclusions The age⁃specific incidence and mortality of malignant tumors in Hainan province showed an increasing trend in 2017, and the affected population developed gradually towards younger. The incidence and mortality of males were higher than those of females. The incidence in urban areas was higher than in rural areas, while the mortality showed an opposite trend. Cancers such as liver cancer, lung cancer, colorectal cancer, breast cancer, and gastric cancer are the focus of prevention and treatment , the prevention and control needs to be further strengthened. Related Articles | Metrics

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Chidamide combined with azacitidine in treating relapsed/refractory angioimmunoblastic T⁃cell lymphoma: a prospective clinical study WEI Chong, ZHAO Danqing, ZHANG Yan, WANG Wei, ZHANG Wei, ZHOU Daobin 2023, 15 (1):  51-55.  doi: 10.3969/j.issn.1674?5671.2023.01.08 Abstract ( 257 )   PDF   Save Objective To investigate the efficacy and safety of chidamide combined with azacitidine in treating relapsed/refractory angioimmunoblastic T⁃cell lymphoma (AITL). Methods The study was a single⁃center prospective clinical trial at Peking Union Medical College. The eligible patients were pathologically confirmed with AITL and met the criteria for relapsed/refractory lymphoma. Treatment included subcutaneous injection of azactidine azacitidine (100 m/d for 7 d) combined with oral chidamide (20 mg/d twice weekly) in a 4 weeks cycle. Results A total of 7 patients were enrolled from March 2021 to August 2022. The median age of disease onset was 53 (range 45-75) years and the median line of previous treatment was 2 (range 1-4) line. After treatment, complete remission (CR) was achieved in 2 cases and partial remission in 3 cases. The objective response rate was 71.4% and the CR rate was 28.6%. As of August 2022, the median follow⁃up time was 14 months (range 4-17 months). 6 patients survived and 1 patient died, and the median progression⁃free survival (PFS), duration of response, and overall survival (OS) of the whole group were not reached. The 1⁃year PFS and OS rates were 51.4% and 100.0%, respectively. Treatment⁃related adverse events were mainly hematological toxicity. The incidence of grade 3-4 neutropenia and thrombocytopenia were 28.6% (2/7) and 14.3% (1/7), respectively. Conclusions Azacitidine combined with chidamide has a high remission rate and safety profile in treating relapsed/refractory AITL, and dual epigenetic regulating drugs may serve as a second⁃line treatment option for patients with AITL. Related Articles | Metrics

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Analysis of  occurrence and risk factors of second malignant neoplasms in children, adolescents and young adult patients with Hodgkin lymphoma based on SEER database JIN Jiayue, REN Simeng, HAN Baojin, ZHANG Wenzheng, XU Hongkun, LIU Jie 2023, 15 (1):  56-61.  doi: 10.3969/j.issn.1674-5671.2023.01.09 Abstract ( 215 )   PDF   Save 霍奇金淋巴瘤；第二原发恶性肿瘤；年轻患者；危险因素 Related Articles | Metrics

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Association of genetic variants in eukaryotic translation initiation factor 3 and risk of HBV⁃related hepatocellular carcinoma ZHAN Shicheng, CHEN Peiqin, QIU Moqin, WEN Qiuping, ZHOU Zihan, LIN Qiuling, JIANG Yanji, ZHOU Xianguo, CAO Ji, GUO Qian, NONG Cunli, YU Hongping, LIU Yingchun 2023, 15 (1):  62-69.  doi: 10.3969/j.issn.1674?5671.2023.01.10 Abstract ( 104 )   PDF   Save Objective To investigate the association of genetic variants in eukaryotic translation initiation factor 3 (eIF3) and the risk of hepatitis B virus⁃related hepatocellular carcinoma(HBV⁃HCC). Methods A two⁃stage case⁃control study was performed in this study. The single nucleotide polymorphisms (SNP) loci associated with HCC risk were selected in the discovery stage included 966 HCC cases and 1003 hepatitis B surface antigen (HBsAg) positive controls from Guangxi. In the validation stage, the  480 HCC cases and 484 HBsAg positive controls from Shanghai were used to validate the positive loci in the discovery stage. The association between genetic variants of eIF3 and the risk of HBV⁃HCC was analyzed by univariable and multivariable logistic regression. Results The results showed that two potential functional SNPs (rs7401 A>G and rs23057952 A>G) in 3'⁃UTR of EIF3G were associated with the risk of HBV⁃HCC. Compared with the A allele carriers, rs7401 G allele carriers had an increased risk of HBV⁃HCC (OR=1.18, 95%CI: 1.02-1.38, P=0.028). While, rs2305795 G allele reduced the risk of HBV⁃HCC compared with the A allele (OR=0.74, 95%CI: 0.64-0.86, P<0.001). Combined analysis showed a dose⁃response manner between the number of unfavorable genotypes (rs7401 AG/GG genotypes and rs2305795 AA genotype) and the increased risk of HBV⁃HCC (Ptrend<0.001). Expression quantitative trait loci (eQTL) analysis revealed that both rs7401 G allele and rs2305795 A allele were significantly associated with increased EIF3G mRNA expression level, which were significantly higher in HCC tissues than in normal tissues. Conclusions EIF3G rs7401 G allele and EIF3G rs2305795 A allele can increase the risk of HBV⁃HCC. Related Articles | Metrics

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Analysis of the mediating effect of self⁃representation in alexithymia and fear of disease progression in patients with nasopharyngeal carcinoma XU Yongrong, YANG Li, MA Lingxia, HUANG Xia, GAN Jiemei 2023, 15 (1):  70-74.  doi: 10.3969/j.issn.1674?5671.2023.01.11 Abstract ( 99 )   PDF   Save Objective To investigate the mediating role of self⁃representation in alexithymia and fear of disease progression in patients with nasopharyngeal carcinoma. Methods A total of 241 patients with nasopharyngeal carcinoma, who attended the Department of Medical Oncology of the First Affiliated Hospital of Guangxi Medical University from December 2020 to June 2021, were selected as the research objects. The distress disclosure index (DDI), the Toronto alexithymia scale (TAS) and the fear of progression questionnaire⁃short form (FoP⁃Q⁃SF) were used to analyze the correlation between the alexithymia, self⁃representation and the fear of disease progression in patients with nasopharyngeal carcinoma, and to analyze the mediating role of self⁃representation in alexithymia and fear of disease progression. Result The total scores of self⁃representation, fear of disease progression and alexithymia were (29.39±8.26) points, (34.51±5.68) points and (54.67±8.91) points, respectively, in patients with nasopharyngeal carcinoma. The results of Pearson correlation analysis showed that the total scores of alexithymia was negatively correlated with self⁃representation (P<0.001), and positively correlated with fear of disease progression (P<0.001). Self⁃representation was negatively correlated with fear of disease progression (P<0.001). The results of mediation analysis showed that there was a partial mediating effect of self⁃representation between alexithymia and fear of disease progression (the mediating effect accounted for 21.6%). Conclusions Patients with nasopharyngeal carcinoma are less willing to self⁃representation, and alexithymia and fear of disease progression need to be improved. Alexithymia can have an impact on patients' fear of disease progression through the mediation of self⁃representation. Related Articles | Metrics

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Analysis of the registration status of osteosarcoma clinical trial based on ClinicalTrials.gov database LIANG Yuting, WEI Suping, WANG Yu, XIE Xing, LI Ruolin, LING Zhi'an 2023, 15 (1):  75-80.  doi: 10.3969/j.issn.1674-5671.2023.01.12 Abstract ( 169 )   PDF   Save Objective To analyze the global registration of osteosarcoma clinical trials, to understand the research status and trend of the field, and to provide the latest information for osteosarcoma research and clinical diagnosis and treatment. Methods The data of osteosarcoma clinical trials registered in ClinicalTrials.gov from 2001 to 2021 were collected and statistically analyzed in terms of regional distribution, annual trend, trial progress, research type, clinical stage, and number of participating institutions. Results From 2001 to 2021, a total of 514 global osteosarcoma clinical trials were registered, mainly in North America and Europe. Among them, 416 (80.9%) were interventional clinical trials, 87.0% (362/416) were drug⁃related treatments, and 127 were anti⁃tumor drug studies. The results showed that only 147 (35.3%) interventional clinical trials had been completed, including 71 (48.3%) published papers and 33 (46.5%) published trial results. Compared with 2001-2011, the proportion of interventional clinical trials for osteosarcoma registered in Asia increased from 6.1% (10/163) to 15.0% (38/253) between 2012 and 2021, and the proportion of pre⁃recruitment registrations increased from 1.2% (2/163) to 43.9% (111/253), However, the number of phase Ⅱ clinical trials still accounted for the largest proportion in these two periods, which were 46.0% (75/163) and 37.9% (96/253), respectively. Conclusions The majority of global osteosarcoma studies are interventional clinical trials, mainly focusing on drug⁃related treatments, and antitumor drugs are still the first choice for the osteosarcoma drug intervention research. The number of osteosarcoma interventional clinical trials registered in Asia has increased significantly, but the quality and quantity of these researches are still quite different from those in North America and Europe. Related Articles | Metrics

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esearch progress of OGF and OGFr in common gastrointestinal malignancies ZHANG Mingying, YANG Xiaodong, XU Yanming, WANG Ming, YAN Yuye, YU Qiuyi, GAO Jing, WANG Shubin 2023, 15 (1):  80-84.  doi: 10.3969/j.issn.1674-5671.2023.01.13 Abstract ( 204 )   PDF   Save Related Articles | Metrics

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Effect of viral infection on antitumor therapy in patients with malignant tumors and antiviral strategies LI Wei, MA Shishi, CHEN Xiaopin 2023, 15 (1):  85-91.  doi: 10.3969/j.issn.1674?5671.2023.01.14 Abstract ( 80 )   PDF   Save Related Articles | Metrics

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Research progress of the correlation between bladder cancer and urinary flora FAN Liang, CUI Zhenyu, WANG Yaqiong, YANG Wenzeng 2023, 15 (1):  92-97.  doi: 10.3969/j.issn.1674?5671.2023.01.15 Abstract ( 83 )   PDF   Save Related Articles | Metrics

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Advances in the management of symptom clusters in patients with multiple myeloma IN Yuxuan, LIU Wei, YANG Hui, DING Cuiping 2023, 15 (1):  98-103.  doi: 10.3969/j.issn.1674-5671.2023.01.16 Abstract ( 172 )   PDF   Save Related Articles | Metrics

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Malignant peritoneal mesothelioma treated with immune checkpoint inhibitor combined with systemic chemotherapy and heated intraperitoneal chemotherapy: a case report QUAN Xiaoying, CHEN Xiaoyan, LEI Lei, JIA Xiaoli, WU Chunzhi, YE Bin 2023, 15 (1):  104-106.  doi: 10.3969/j.issn.1674?5671.2023.01.17 Abstract ( 76 )   PDF   Save Related Articles | Metrics