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中国癌症防治杂志

中国癌症防治杂志 ›› 2012, Vol. 4 ›› Issue (1): 42-45.doi: 10.3969/j.issn.1674-5671.2012.01.10

• 基础研究 • 上一篇    下一篇

单肺通气大鼠肺泡表面活性蛋白表达的研究易

  

  1. 广西医科大学附属肿瘤医院麻醉科；广西医科大学研究生学院
  • 出版日期:2012-03-25 发布日期:2012-04-16
  • 通讯作者: 黄冰 E-mail:hhhbing@163.com
  • 基金资助:

    广西自然科学基金资助项目（桂科自 NO 0832232）

Changes in pulmonary surfactant proteins during one lung ventilation in rats

  • Online:2012-03-25 Published:2012-04-16

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摘要： 目的 研究单肺通气（OLV）大鼠肺组织肺泡表面活性蛋白A（SP-A）和肺泡表面活性蛋白B（SP-B）表达的变化。方法 42只SD大鼠随机分成单肺通气组（A组）、双肺通气对照组（B组）和空白对照组（C组）。A组和B组根据不同的通气时间分成亚组A1、A2、A3组和B1、B2、B3组，将气管导管过深插管至右肺建立右肺OLV模型。分别取各组左右两侧肺组织，光镜下观察其肺泡结构及肺间质的病理变化，免疫印迹法检测肺组织中SP-A和SP-B蛋白水平。结果 与C组相比较，随着通气时间的延长，A组和B组肺组织逐渐表现出肺泡充血水肿、肺间质逐渐增厚，且在A组非通气侧这些病理变化更为严重。与C组比较，A组各亚组SP-A和SP-B蛋白表达均减少（P＜0.05），且随着OLV时间延长，蛋白表达减少更加明显（P＜0.05）；与B组各亚组对应侧肺比较，A组中各亚组左侧肺SP-A和SP-B蛋白表达均减少（P＜0.05），右侧肺SP-A蛋白表达差异无统计学意义（P＞0.05），右侧肺SP-B蛋白表达在A1、A2组差异无统计学意义（P＞0.05），而在A3组则比B3组蛋白表达减少（P＜0.05）；A组各亚组组内两侧肺比较，SP-A和SP-B蛋白在A组各亚组左侧肺均比右侧肺蛋白表达减少（P＜0.05）。结论 OLV可致SP-A和SP-B表达下调，且非通气侧比相同时间的双肺通气肺组织下调更明显，这可能是OLV所致肺损伤更加严重的重要因素。

关键词: 单肺通气, 肺泡表面活性蛋白A, 肺泡表面活性蛋白B

Abstract: Objective To study changes in pulmonary surfactant proteins A （SP-A） and B （SP-B） in rat lung tissue after one lung ventilation.Methods Sprague-Dawley rats （n=42） were randomly divided into three groups：one lung ventilation group （group A），two lung ventilation group （group B），and control group （group C）.Groups A and B were further subdivided depending on the ventilation time （A1，A2，A3；B1，B2，B3）.An artificial endotracheal tube was pushed into the right main bronchus in order to perform one lung ventilation.Pathological changes in the lung were examined by light microscopy，and the expression of SP-A and SP-B in lung tissues was detected by Western blot.Result In contrast to group C，groups A and B showed intra-alveolar exudates，hemorrhaging and interstitial edema that was proportional to ventilation duration.The left （unventilated） lung showed more serious damage than the right lung in group A.Levels of SP-A and SP-B were significantly lower in group A than in group C（P＜0.05），and the levels in group A decreased with increasing OLV duration（P＜0.05）. In the left lung，levels of SP-A and SP-B were significantly lower in A1，A2，and A3 than in B1，B2，and B3，respectively（P＜0.05）. In the right lung，there were no significant differences between groups A and B in the expression of SP-A （P＞0.05），nor were there significant differences between A1 and B1 or A2 and B2 in the expression of SP-B （P＞0.05）.However，the levels of SP-B were lower in A3 than in B3 （P＜0.05）.The levels of SP-A and SP-B were lower in the left lung than in the right one in A1，A2，and A3（P＜0.05）.Conclusion OLV causes the levels of SP-A and SP-B in lung tissue to decrease below the levels observed with two lung ventilation of the same duration.This may be a primary cause of the more serious lung injury caused by OLV.

Key words: One lung ventilation, Pulmonary surfactant protein A, Pulmonary surfactant protein B