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中国癌症防治杂志

中国癌症防治杂志 ›› 2016, Vol. 8 ›› Issue (5): 278-282.doi: 10.3969/j.issn.1674-5671.2016.05.03

• 临床研究 • 上一篇    下一篇

NF-κB、COX-2、LKB1在子宫内膜癌组织中的表达及临床意义

  

  1. 广西医科大学附属肿瘤医院妇瘤科二病区；广西医科大学研究生学院
  • 出版日期:2016-10-25 发布日期:2016-11-14
  • 通讯作者: 宋红林 381783170@qq.com
  • 基金资助:

    广西自然科学基金资助项目（2011GXNSFA018253）

NF-κB，COX-2 and LKB1 expression in endometrial cancer tissues and clinical significance

  • Online:2016-10-25 Published:2016-11-14

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摘要：

目的 探讨核转录因子κB（NF-κB）、环氧化酶2（COX-2）、肝激酶基因B1（LKB1）在不同子宫内膜组织中的表达及临床意义。方法 采用免疫组化SP法检测60例正常子宫内膜、31例非典型增生子宫内膜和60例子宫内膜癌组织中NF-κB、COX-2、LKB1蛋白的表达，并分析其与子宫内膜癌临床病理特征的关系。结果 NF-κB、COX-2、LKB1在子宫内膜癌组织、非典型增生子宫内膜组织和正常子宫内膜组织中的阳性表达率分别为63.3%、70.0%、35.0%，41.9%、64.5%、61.3%和35.0%、51.7%、93.3%，NF-κB、LKB1在3组间的表达差异有统计学意义（P＜0.05），而COX-2的表达差异无统计学意义（P＞0.05）。子宫内膜癌中NF-κB、COX-2、LKB1蛋白的阳性表达与病理类型、FIGO分期、淋巴结转移均无关（P＞0.05）。LKB1蛋白表达与肌层浸润深度和组织学分级相关（P＜0.05），NF-κB蛋白表达与组织学分级亦相关（P＜0.05）。结论 NF-κB在子宫内膜癌组织中高表达、LKB1低表达，其表达可能与子宫内膜癌的发生发展有关。

关键词: 子宫肿瘤, 子宫内膜癌, NF-&kappa, B, COX-2, LKB1, 免疫组织化学法, 蛋白表达, 临床意义

Abstract:

Objective To analyze NF-κB，COX-2 and LKB1 expression in endometrial cancer tissues and normal controls，and assess clinical significance. Methods Immunohistochemistry was used to analyze expression of NF-κB，COX-2 and LKB1 in 60 endometrial cancer tissues，60 normal endometrial tissues from uterine myomas and 31 cases of atypical hyperplasia of endometrium. Possible relationships were explored between NF-κB，COX-2 and LKB1 expression and clinicopathological variables. Results The rate of NF-κB positivity was 63.3% for endometrial cancer tissue，41.9% for normal endometrial tissue，and 35.0% for atypical hyperplasia tissue. The corresponding results for COX-2 positivity were 70.0%，64.5% and 51.7%. The corresponding results for LKB1 positivity were 35.0%，61.3% and 93.3%. Expression of NF-κB and LKB1 varied significantly across the three types of tissue（P＜0.05），but expression of COX-2 did not（P＞0.05）. Expression of none of the three proteins correlated with pathology type，clinical stage or lymph node metastasis；however，LKB1 expression correlated positively with myometrial invasion and histology grade（P＜0.05），while NF-κB expression correlated with histology grade（P＜0.05）. Conclusion NF-κB is over-expressed and LKB1 under-expressed in endometrial cancer tissue. These proteins may be involved in the development and progression of endometrial cancer.

Key words: Uterine neoplasms, Endometrial cancer, NF-κB, COX-2, LKB1, Immunohistochemistry, Proten expression, Clinical significance