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中国癌症防治杂志

中国癌症防治杂志 ›› 2020, Vol. 12 ›› Issue (3): 303-309.doi: 10.3969/j.issn.1674-5671.2020.03.12

• 基础研究 • 上一篇    下一篇

PES1对肝细胞癌预后和肝癌BEL-7402细胞生长的影响

  

  1.  空军军医大学第二附属医院疼痛科，神经内科
  • 出版日期:2020-06-25 发布日期:2020-07-12
  • 通讯作者: 袁鹏 E-mail：yuanpeng834700@126.com
  • 基金资助:
    国家自然科学基金项目（81772934；81802345）；中国博士后科学基金项目（2019M663984）

Effects of PES1 on the prognosis of hepatocellular carcinoma and the growth of hepatocellular carcinoma BEL-7402 cells

  • Online:2020-06-25 Published:2020-07-12

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摘要： 目的 探讨pescadillo核糖体生物发生因子1（pescadillo ribosomal biogenesis factor 1，PES1）在肝细胞癌（hepatocellular carcinoma，HCC）组织中的表达及其对肝癌BEL-7402细胞生长的影响。方法 采用免疫组化法检测HCC癌组织和癌旁正常组织中PES1的表达。肝癌BEL-7402细胞转染PES1小干扰片段后，采用Western blot检测PES1、CDK4、CDC25A蛋白表达，MTS法检测细胞生长情况，流式细胞术检测细胞周期和细胞凋亡。结果 95例HCC癌组织中，PES1表达阳性率高于癌旁正常组织（90.5% vs 72.6%，χ2=10.122，P=0.001）；癌组织中PES1染色评分高于癌旁正常组织的［（6.30±3.56） 分 vs （2.10±1.64） 分，t=10.423，P<0.001］。PES1表达与HCC患者的肿瘤大小、门脉癌栓和TNM分期有关（均P<0.05）；PES1高表达患者总生存期较PES1低表达患者短（P<0.05）。转染PES1小干扰RNA组的细胞与对照组相比，细胞生长受抑制（P<0.01），G1期细胞比例增加，S期细胞比例减少（均P<0.01），CDK4、CDC25A蛋白表达下调，但细胞凋亡未见明显变化。结论 HCC组织中PES1表达上调，且与患者预后相关。PES1可通过上调CDK4、CDC25A蛋白表达，加快细胞G1/S期的转换，促进肝癌细胞生长。

关键词: 肝细胞癌, PES1, 预后, 细胞生长

Abstract: Objective To investigate the expression of pescadillo ribosomal biogenesis factor 1（PES1） in hepatocellular carcinoma （HCC）and its effect on the growth of hepatocellular BEL-7402 cells. Methods The expression of PES1 in HCC and adjacent normal tissues was detected by immunohistochemical （IHC）staining. The protein expression of PES1，CDK4 and CDC25A were detected by Western blot after BEL-7402 cells were transfected with PES1 siRNA，and the cell growth was detected by MTS，the cells cycle and apoptosis were detected by flow cytometry. Results The positive expression rate of PES1 in 95 HCC tissues was higher than that of the adjacent normal tissues（90.5% vs 72.6%，χ2=10.122，P=0.001），and the IHC staining score of PES1 in HCC tissues was higher than that of the adjacent normal tissues（6.30±3.56 vs 2.10±1.64，t=10.423，P<0.001）. The expression of PES1 was related to tumor size，portal vein tumor thrombus and TNM stage in HCC patients（all P<0.05）. The overall survival of HCC patients with high expression of PES1 were shorter than those with PES1 low expression（P<0.05）. Compared with the control group，the growth of cells in the PES1 small interfering RNA group was inhibited（P<0.01），the proportion of cells in G1 phase increased，and the proportion of cells in S phase decreased （all P<0.05）. The expression of CDK4 and CDC25A protein was down-regulated，but the cells showed no obvious change in apoptosis. Conclusions The expression of PES1 in HCC tissues is up-regulated and relate to the prognosis of HCC patients. PES1 may accelerate the G1/S phase transformation and promote the cells growth by up-regulating the expression of CDK4 and CDC25A.

Key words: Hepatocellular carcinoma, PES1, Prognosis, Cells growth

中图分类号: 

  • R735.7