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中国癌症防治杂志

中国癌症防治杂志 ›› 2020, Vol. 12 ›› Issue (5): 548-554.doi: 10.3969/j.issn.1674-5671.2020.05.12

• 基础研究 • 上一篇    下一篇

 miR-572 通过靶向调控 WWOX 影响肺癌细胞恶性生物学行为

  

  1. 十堰市太和医院(湖北医药学院附属医院）呼吸与危重症医学科；武汉市中心医院呼吸内科
  • 出版日期:2020-10-25 发布日期:2020-10-25
  • 通讯作者: 王梅芳 Ｅ-ｍａｉｌ： yuyuaan@126.comc
  • 基金资助:

     

Influence of miR-572 on the malignant biological behavior of lung cancer cells via targeting WWOX regulation

  1. Department of Respiratory and Critical Care Medicine, Taihe Hospital of Shiyan City, ; Respiratory Medicine Department, Wuhan Central Hospital
  • Online:2020-10-25 Published:2020-10-25
  • Contact: WANG Meifang E-mail: yuyuaan@126.com

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摘要： 目的 探讨miR-572靶向氧化还原酶的WW结构域（WW domain containing oxidoreductase，WWOX）调控肺癌细胞增殖、凋亡的分子机制。方法 选取50例2016年3月—2018年5月十堰市太和医院手术切除肺癌组织及其相应的癌旁组织。利用脂质体转染技术将miR-572 inhibitor和miR-572 mimics转染至肺癌A549和L9981细胞；采用qRT-PCR检测miR-572和WWOX的mRNA表达水平，MTT法检测细胞增殖能力，流式细胞仪检测细胞周期和细胞凋亡情况。通过生物信息学软件Targetscan分析miR-572的靶基因，荧光素酶报告基因实验检验miR-572和WWOX的靶向关系。结果 miR-572在肺癌组织和细胞中高表达（均P<0.05）。下调miR-572后，肺癌细胞A549、L9981中miR-572表达水平均降低（均P<0.05），细胞增殖能力也降低（均P<0.05），细胞G0/G1期比例和细胞凋亡率均升高（均P<0.05）；而上调miR-572后，肺癌A549、L9981细胞增殖能力升高（均P<0.05），细胞G0/G1期比例和细胞凋亡率则降低（均P<0.05）。WWOX在肺癌组织和细胞中低表达（均P<0.05），且在肺癌组织中WWOX与miR-572表达呈负相关（r=-0.669，P<0.001）。下调WWOX可逆转下调miR-572对肺癌细胞的增殖抑制、周期阻滞和凋亡促进作用。 结论 下调miR-572可抑制肺癌细胞增殖并诱导细胞凋亡，其作用机制可能与靶向负调控WWOX有关。

关键词: 肺癌, 增殖, 凋亡, miR-572, 氧化还原酶的WW结构域

Abstract: Objective To investigate the molecular mechanism of miR-572 targeting WW domain containing oxidoreductase(WWOX) in regulating the proliferation and apoptosis of lung cancer cells. Methods A total of 50 cases of lung cancer tissues and their corre-sponding adjacent tissues of lung cancer patients who underwent surgical resection in Taihe Hospital of Shiyan Gity from March 2016 to May 2018 were collected. miR-572 inhibitor and miR-572 mimics were transfected into lung cancer A549 and L9981 cells by using liposome transfection technology. The expressions of miR-572 and WWOX mRNA were determined by qRT-PCR, the cell proliferation was measured by MTT assay, and the cell cycle and apoptosis were measured by flow cytometry. Targetscan was used to analyze the target genes of miR-572, the luciferase reporter gene experiment confirmed the targeting relationship between miR-572 and WWOX. Results miR-572 was highly expressed in lung cancer tissues and cells ( all P<0.05). After down-regulating miR-572, the proliferation ability of lung cancer A549 and L9981 cells decreased(all P<0.05), but the cell G0/G1 phase ratio and apoptosis rate increased(all P<0.05). However, after up-regulating miR-572, the proliferation ability of miR-572 in lung cancer A549 and L9981 cells increased (all P<0.05), while the cell G0/G1 phase ratio and apoptosis rate of cells decreased(all P<0.05). WWOX was low expressed in lung cancer tissues and cells( all P<0.05), and it was negatively correlated with the expression of miR-572 in lung cancer tissues(r=-0.669, P<0.001). Down-regulating the expression of WWOX could reverse the effect of miR-572 on lung cancer cell proliferation inhibition, cycle arrest and apoptosis promotion. Conclusion Down-regulation of miR-572 can inhibit the proliferation and induce apoptosis of lung cancer cells, probably through targeting negative regulation of WWOX.

Key words: Lung cancer, Proliferation, Apoptosis, miR-572, WW domain containing oxidoreductase

中图分类号: 

  • R734.2