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中国癌症防治杂志

中国癌症防治杂志 ›› 2021, Vol. 13 ›› Issue (5): 458-463.doi: 10.3969/j.issn.1674-5671.2021.05.03

• 肝癌专栏 • 上一篇    下一篇

短串联重复序列分型鉴定肝癌肝移植术后超晚期复发肿瘤起源的可行性研究

  

  1. 复旦大学附属中山医院肝肿瘤外科，复旦大学肝癌研究所，癌变与侵袭原理教育部重点实验室
  • 出版日期:2021-10-25 发布日期:2021-11-16
  • 通讯作者: 周俭 E-mail：zhou.jian@zs-hospital.sh.cn
  • 基金资助:
    国家重点研发计划项目（2019YFC1315800；2019YFC1315802）；国家自然科学基金项目（82150004；81830102；81772578； 81802991）；复旦大学原创科研个性化支持项目（IDF152064/014）；复旦大学附属中山医院优秀青年计划（2021ZSYQ09）；上海市临床重点专科项目经费资助

Feasibility study of short tandem repeat genotyping to identify the origin of ultra?late recurrence of liver cancer after liver transplantation

  • Online:2021-10-25 Published:2021-11-16

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摘要： 目的 分析短串联重复序列（short tandem repeat，STR）分型鉴定肝癌肝移植术后超晚期复发肿瘤起源的可行性。方法 回顾性收集本院1例肝癌肝移植术后发生超晚期肿瘤复发患者的原发灶和复发灶样本，采用PCR技术体外扩增15个STR基因座片段，通过分析病灶STR位点一致性进行鉴定。结果 STR基因座的检测和对比结果显示，复发灶中的15个STR位点（D3S1358、vWA、D8S1179、D21S11、D18S51、D2S441、D19S433、TH01、FGA、D22S1045、D5S818、D13S317、D10S1248、D1S1656、D12S391）与原发灶相同，似然率为2.28×1018，因此排除了复发灶为供肝新生肿瘤的可能性，证实其为原发灶起源的复发肿瘤。结论 STR分型可以有效地对肝癌肝移植术后超晚期复发肿瘤的起源进行鉴定。

关键词: 肝细胞癌, 同种异体原位肝移植, 超晚期复发, 短串联重复序列分型

Abstract: Objective To analyze the feasibility of identifying the origin of ultra-late recurrence of liver cancer after liver transplantation  by short tandem repeat (STR) genotype. Methods The primary and recurrent lesion samples of one patient with ultra-advanced tumor recurrence after orthotopic liver transplantation in Zhongshan Hospital, Fudan University, were retrospective collected. 15 STR loci were amplified by PCR in vitro, and identified by analyzing the consistency of STR loci. Results The detection and comparison results of STR loci showed that 15 STR loci (D3S1358, vWA, D8S1179, D21S11, D18S51, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D10S1248, D1S1656, and D12S391) in recurrent lesions were identical to those in primary lesions. with 2.28×1018 likelihood ratio. Thus, the possibility that the recurrent lesions was a new tumor was ruled out, and instead it was confirmed to be the recurrent tumor from primary lesion. Conclusions STR genotyping technology can effectively identify the origin of ultra-late recurrence after liver transplantation.

Key words: Hepatocellular carcinoma, Orthotopic liver transplantation, Ultra-late recurrence, Short tandem repeat genotyping

中图分类号: 

  • R735.7