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中国癌症防治杂志

中国癌症防治杂志 ›› 2024, Vol. 16 ›› Issue (2): 193-199.doi: 10.3969/j.issn.1674-5671.2024.02.09

• 临床研究 • 上一篇    下一篇

胃癌发生过程中风险miRNAs筛选及其诊断效能评价的多中心研究

  

  1. 广西医科大学第一附属医院肿瘤内科；广西医科大学附属肿瘤医院内镜中心；桂林市人民医院消化内科；右江民族医学院附属医院消化内科
  • 出版日期:2024-04-25 发布日期:2024-05-08
  • 通讯作者: 葛莲英 E-mail：gelianying2008@163.com
  • 基金资助:
    广西重点研发计划项目（桂科AB20297021；桂科AB18221084）

Screening and diagnostic efficacy evaluation of risk miRNAs during the development of gastric cancer: a multicenter study

  • Online:2024-04-25 Published:2024-05-08

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摘要： 目的 探索胃癌发生过程的风险miRNAs，为上消化道机会性筛查中早期胃癌识别提供依据。 方法 纳入2021年6月至2023年8月在广西医科大学附属肿瘤医院、右江民族医学院附属医院、桂林市人民医院3个中心进行上消化道癌机会性筛查的人群。选取健康体检者107例、早期胃癌患者71例、进展期胃癌患者97例。首先采用转录组测序筛选差异表达miRNAs，然后在3组前瞻性人群的血浆样本中通过RT⁃qPCR验证差异表达的miRNAs，最后采用受试者工作特征（receiver operating characteristic，ROC）曲线评估miRNAs的诊断效能。 结果 转录组测序的差异基因分析共筛选出胃癌发生过程中的6个差异表达miRNAs，包括miR⁃3176、miR⁃885⁃5p、miR⁃203a⁃3p、miR⁃452⁃5p、miR⁃223⁃3p、miR⁃219a⁃2⁃3p。RT⁃qPCR结果显示，miR⁃452⁃5p在早期胃癌及进展期胃癌患者中表达上调（均P<0.001）。 ROC曲线显示，miR⁃452⁃5p诊断早期胃癌和进展期胃癌的曲线下面积（area under the curve，AUC）分别为0.900、0.975。区分早期胃癌与进展期胃癌的AUC为0.843。结论 miR⁃452⁃5p在早期胃癌中具有良好的诊断效能，可能作为液体活检诊断早期胃癌的潜在生物标志物。

关键词: 早期胃癌, miR?452?5p, 生物标志物

Abstract: Objective To explore the risk miRNAs during the development of gastric cancer, providing the evidence for early gastric cancer identification in upper gastrointestinal opportunistic screening. Methods The patients who underwent opportunistic screening population for upper gastrointestinal cancer in the Guangxi Medical University Cancer Hospital, the Affiliated Hospital of Youjiang Medical University for Nationalities, and Guilin People's Hospital from June 2021 to August 2023 were enrolled. A total of 107 healthy individuals, 71 patients with early gastric cancer, and 97 patients with advanced gastric cancer were selected. The differential expression of miRNAs was screened by using transcriptome sequencing, and the differential expression of miRNAs was validated by RT⁃qPCR in prospective blood samples of the 3 prospective groups. The diagnostic efficacy of miRNAs was evaluated by using receiver operating characteristic (ROC) curves. Results The differential gene analysis by transcriptome sequencing screened out 6 differentially expressed miRNAs in the development of gastric cancer development, including miR⁃3176, miR⁃885⁃5p, miR⁃203a⁃3p, miR⁃452⁃5p, miR⁃223⁃3p, and miR⁃219a⁃2⁃3p. RT⁃qPCR results showed that miRNA⁃452⁃5p was up⁃regulated in both the early gastric cancer and the advanced gastric cancer (all P<0.001). The ROC curves indicated that the area under the curve (AUC) of miRNA⁃452⁃5p for diagnosing early gastric cancer and advanced gastric cancer were 0.900 and 0.975, respectively. The AUC for distinguishing early gastric cancer and advanced gastric cancer was 0.843. Conclusions miRNA⁃452⁃5p demonstrates a good diagnostic efficacy in early gastric cancer, and may serve as a potential biomarker for the diagnosis of early gastric cancer by liquid biopsy.

Key words: Early gastric cancer, miRNA?452?5p, Biomarkers

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  • 引用本文

    马福超, 刘爱群, 谢为舜, 沈妍华, 冯洁, 蒋旗, 葛莲英. 胃癌发生过程中风险miRNAs筛选及其诊断效能评价的多中心研究[J]. 中国癌症防治杂志, 2024, 16(2): 193-199.

    MA Fuchao, LIU Aiquan, XIE Weishun, SHEN Yanhua, FENG Jie, JIANG Qi, GE Lianying. Screening and diagnostic efficacy evaluation of risk miRNAs during the development of gastric cancer: a multicenter study[J]. Chinese Journal of Oncology Prevention and Treatment, 2024, 16(2): 193-199.