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中国癌症防治杂志

中国癌症防治杂志 ›› 2011, Vol. 3 ›› Issue (3): 192-193.doi: 10.3969/j.issn.1674-5671.2011.03.03

• 基础研究 • 上一篇    下一篇

比较基因组杂交技术检测肝细胞癌染色体异常及其临床意义

肖开银, 彭民浩, 秦权林, 桂文波, 覃晓, 彭涛, 郭雅, 陈滨, 卢景宁, 黎乐群   

  1. 广西医科大学第一临床医学院肝胆外科
  • 出版日期:2011-09-25 发布日期:2011-10-17
  • 通讯作者: 黎乐群 E-mail:li_lequn@263.net
  • 基金资助:

    广西科技厅科学基金资助课题（桂科自0728145，桂科自0229026）

Clinical signification of chromosomal aberration in hepatocellular carcinoma determined by comparative genomic hybridization

XIAO Kai-Yin, PENG Min-Hao, QIN Quan-Lin, GUI Wen-Bo, QIN Xiao, PENG Tao, GUO Ya, CHEN Bin, LU Jing-Ning, LI Le-Qun   

  • Online:2011-09-25 Published:2011-10-17

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摘要： 目的 探讨肝细胞癌染色体异常及其临床意义。方法 运用比较基因组杂交技术检测25例肝细胞癌染色体DNA异常情况，并与临床指标作相关分析。结果 25例肝癌均存在不同程度的染色体DNA拷贝数的扩增或缺失，较为常见的染色体DNA异常是+1q(72%)、+1p(64%)、+2q(48%)、+2p(48%)、+5q(48%)、+Xq(48%)、+7q(44%)、-4q(48%)、-16p(48%)、-8p(40%)、-17p(36%)；相关分析显示+17p、+18p、-8p、-13q、-11q、-8q染色体异常事件与临床指标部分相关。结论 肝细胞癌存在明显的染色体异常，部分染色体异常事件是非随机性的，可能与肝癌的发生、发展有关，并与肿瘤的生物学行为和预后相关。

关键词: 肝肿瘤, 肝细胞癌, 比较基因组杂交, 染色体异常, 预后

Abstract: Objective To screen chromosomal aberration in hepatocellular carcinoma (HCC) and to explore the clinical signification.  Methods  Chromosomal aberration was detected by comparative genomic hybridization (CGH) in 25 patients with HCC，and the correlation between chromosomal aberration and clinical parameters were analyzed. Results All patients presented certain aberrations in copy number of chromosomal DNA. The amplification of copy number was commonly detected in chromosome 1q (72.0%)，1p (64.0%)，2q (48.0%)，2q (48.0%)，5q (48.0%) and 7q (44.0%)，while the loss of copy number was often found in chromosome 4q (48.0%)，16p (48.0%)，8p (40.0%) and 17p (36.0%).  The amplification of 17p and 18p as well as the loss of 8p，13q，11q and 8q showed significant correlation with some clinical parameters. Conclusions Chromosomal aberrations occur frequently in HCC. Some of the nonrandom chromosomal alterations may relate to the development，biological behavior and prognosis of HCC.

Key words: Liver neoplasm, Hepatocellular carcinoma (HCC), Comparative genomic hybridization (CGH), Chromosomal aberration, Prognosis of HCC