微信公众号

官网二维码
中国癌症防治杂志

中国癌症防治杂志 ›› 2014, Vol. 6 ›› Issue (1): 12-16.doi: 10.3969/j.issn.1674-5671.2014.01.03

• 基础研究 • 上一篇    下一篇

人肝癌细胞系中CD90+细胞的生物学特性

  

  1. 广西肿瘤防治研究所实验研究部；广西医科大学研究生学院
  • 出版日期:2014-03-25 发布日期:2014-04-10
  • 通讯作者: 张力图 E-mail:zhanglitu@gmail.com
  • 基金资助:

    广西科学研究与技术开发计划课题（桂科攻 1355005-3-10）；广西医科大学青年科学基金项目（GXMUYSF201215）

Biological Characteristics of CD90+ Cells in Human Hepatocellular Carcinoma Cell Lines

  • Online:2014-03-25 Published:2014-04-10

PDF (PC)

697

赞

摘要： 目的 筛选肝癌细胞系中CD90 （Thy-1）阳性表达的细胞，初步探讨CD90+细胞亚群的干细胞特性。方法 利用流式细胞分选术筛选5种肝癌细胞株（HepG2、SMMC-7721、Bel-7404、Bel-7403及SK-Hep-1）中CD90 （Thy-1）阳性表达的细胞，以平板克隆形成实验观察其增殖能力，CCK-8法观察CD90+细胞的增殖能力和顺铂对CD90+细胞的抑制率。结果 肝癌细胞株Sk-Hep-1中有66.02%的细胞CD90呈阳性表达。平板克隆形成实验显示CD90+细胞克隆形成率为（18.60±2.95）%，显著高于CD90-细胞及未分选细胞，差异有统计学意义（P＜0.05）。体外实验显示培养4 d后CD90+细胞的增殖较CD90-及未分选细胞明显加快；CD90+细胞在相同浓度的顺铂作用下其抑制率较CD90-细胞和未分选细胞明显降低，三者的抑制率分别为14.8%、29.7%和24.0%，差异有统计学意义（P＜0.05）。结论 肝癌细胞中的CD90+细胞是具有肿瘤干细胞特性的细胞亚群，CD90可能是肝癌干细胞候选的分子标志物。

关键词: 肝肿瘤, 流式细胞分选术, CD90, Thy-1, 干细胞, 顺铂, 抗药性,肿瘤, 生物学特性

Abstract: Objective To isolate CD90+ （Thy-1+） cells from human hepatocellular carcinoma （HCC） cell lines and to study their stem cell properties. Methods Fluorescence-activated cell sorting was used to detect CD90 expression in five HCC cell lines：HepG2，SMMC-7721，Bel-7404，Bel-7403 and SK-Hep-1.The tablet clone formation test was used to measure the colony-forming ability of the sorted CD90+ cells，and the CCK-8 assay was used to analyze their proliferation ability and their sensitivity to cisplatin. Results CD90 expression was detected in 66.02% of Sk-Hep-1 cells.The pelletizing rate of these CD90+ cells was（18.6±2.95）% in the tablet colony formation assay， significantly higher than that of CD90- and unsorted cells（P＜0.05）. Proliferation after 4 days in culture was significantly greater for CD90+ cells than for CD90- and unsorted cells.Cisplatin inhibited growth of CD90+ cells by 14.8%，significantly less than it inhibited the growth of CD90- cells （29.7%，P＜0.05） and unsorted cells （24.0%，P＜0.05）. Conclusion CD90+ cells are a subpopulation with tumor stem cell properties.CD90 may be a candidate biomarker for HCC stem cells.

Key words: Hepatocellular neoplasm, Fluorescence-activated cell sorting, CD90, Thy-1, Stem cell, Cisplatin, Drug resistance,neoplasm, Biological characteristics