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中国癌症防治杂志

中国癌症防治杂志 ›› 2017, Vol. 9 ›› Issue (2): 139-142.doi: 10.3969/j.issn.1674-5671.2017.02.11

• 临床经验 • 上一篇    下一篇

肺腺癌组织中ANGPT1 mRNA的表达及临床意义

  

  1. 广西医科大学第一附属医院胸外科；广西医科大学研究生院
  • 出版日期:2017-04-25 发布日期:2017-06-12
  • 通讯作者: 冼磊 xianlei59＠163．ｃｏｍ
  • 基金资助:

    广西自然科学基金资助项目（2013GXNSFAA019270）；广西壯族自治区教育厅高校科研资助项目（YB2014060）

Expression and clinical significance of angiopoietin-1 mRNA in lung adenocarcinoma

  • Online:2017-04-25 Published:2017-06-12

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摘要：

目的 探讨血管生成素1 （angiopoietin-1，ANGPT1）在肺腺癌组织中的表达及其临床意义。方法 采用实时荧光定量PCR法检测ANGPT1 mRNA在51例肺腺癌组织及其相应癌旁正常组织和11例非肿瘤正常肺组织中的表达，分析ANGPT1 mRNA表达与肺腺癌患者临床病理特征的关系。结果 ANGPT1 mRNA在肺腺癌组织中的相对表达量为0.068±0.044，在相应癌旁正常组织中为0.696±0.157，在非肿瘤正常肺组织中为1.158±0.332，三者差异有统计学意义（F=114.34，P＜0.05）。ANGPT1 mRNA表达与肺腺癌患者年龄、性别、分化程度无关（P＞0.05），与淋巴结转移、 TNM 分期有关（P＜0.05) 。结论 ANGPT1 mRNA在肺腺癌组织中呈低表达，与淋巴结转移、 TNM 分期存在相关性，ANGPT1缺失可能与肺腺癌的发生发展有关。

关键词: 肺肿瘤, 肺腺癌, 血管生成素1, 实时荧光定量 PCR

Abstract:

Objective To observe changes in angiopoietin-1（ANGPT1） expression in lung adenocarcinoma and investigate the potential clinical significance. Methods A total of 51 cancer tissue samples and adjacent normal tissues were collected from lung adenocarcinoma patients；in addition，11 non-cancer normal tissues were collected. Relative levels of ANGPT1 mRNA were assessed using quantitative real-time PCR，and possible relationships between ANGPT1 mRNA and clinicopathology were explored. Results Relative levels of ANGPT1 mRNA were 0.068±0.044 in lung adenocarcinoma tissue，0.696±0.157 in adjacent normal tissue，and 1.158±0.332 in non-cancer normal tissue（F=114.34，P<0.05）. Levels of ANGPT1 mRNA in lung adenocarcinoma tissues were not related to gender，age，or degree of differentiation（P>0.05），but they were related to TNM stage and lymphatic metastasis（P<0.05）. Conclusion ANGPT1 expression is reduced in lung adenocarcinoma，which may be involved in disease onset and development.

Key words: Lung neoplasms, Lung adenocarcinoma, Angiopoietin-1, Quantitative real-time PCR