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中国癌症防治杂志

中国癌症防治杂志 ›› 2017, Vol. 9 ›› Issue (3): 181-185.doi: 10.3969/j.issn.1674-5671.2017.03.04

• 基础研究 • 上一篇    下一篇

广西扶绥县肝癌高危人群 HBV S 区基因突变与原发性肝癌的相关性研究

  

  1. 广西医科大学附属肿瘤医院实验研究部；广西医科大学研究生院
  • 出版日期:2017-06-25 发布日期:2017-08-02
  • 通讯作者: 黄天壬 tianrenhuang@sina.com
  • 基金资助:

    国家自然科学基金资助项目（81260319）；广西高校科学技术研究资助项目（KY2015ZD025，ZD2014039，2013ZD016）

A case-control study of association between hepatitis B virus S gene mutation and primary liver cancer

  • Online:2017-06-25 Published:2017-08-02

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摘要：

目的　探讨广西扶绥县肝癌高危人群乙型肝炎病毒（hepatitis B virus，HBV）S区基因突变及其与原发性肝癌（primary liver cancer，PLC）的关系。方法 收集来自扶绥县肝癌高危人群队列30例原发性肝癌患者为病例组，同时在队列中按条件收集30例未发生肝癌的持续HBsAg阳性者为对照组，两组1∶1匹配，收集血清标本。采用巢式PCR方法扩增出HBV S区基因片段，对扩增产物进行测序分析。结果 病例组和对照组突变率差异有统计学意义的突变点为T140A、T140C、T300C、G620A（P＜0.05）；条件logistic回归分析显示，T140C突变可能降低原发性肝癌发病风险（OR=0.484，95%CI：0.239~0.994），T300C突变可能增加原发性肝癌发病风险（OR=2.911，95%CI：1.110~7.629）。结论 与原发性肝癌发生相关的HBV S区基因突变位点有T140A、T140C、T300C和G620A，其中T140C、T300C与原发性肝癌发生有独立相关性。

关键词: 肝肿瘤, 乙型肝炎病毒, S基因, 基因突变, 病例对照研究

Abstract:

Objective To study potential associations between mutations in hepatitis B virus （HBV） S gene and risk of primary liver cancer（PLC） in FuSui county of the Guangxi Zhuang Autonomous Region. Methods A total of 30 new cases of PLC from individuals at high PLC risk in FuSui（based on HBsAg positivity lasting from 2011 to 2015） were matched with 30 controls without cancer. The S gene was amplified from DNA in serum by nested PCR and sequenced directly. Results were analyzed by chi-squared test，Fisher's exact test and conditional logistic regression. Results Frequencies of the mutations T140A，T140C，T300C and G620A differed signifi-cantly between cases and controls（P<0.05）. Logistic regression linked T140C to significantly lower risk of PLC（OR 0.484，95% CI ：0.239-0.994） and T300C with significantly higher risk of PLC（OR 2.911，95% CI ：1.110-7.629）. Conclusion T140C and T300C are independent predictors of PLC，with T140C an apparent protective factor and T300C an apparent risk factor.

Key words: Liver neoplasm, Hepatitis B virus, S gene, Gene mutation, Case-control study