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中国癌症防治杂志

中国癌症防治杂志 ›› 2021, Vol. 13 ›› Issue (3): 248-252.doi: 10.3969/j.issn.1674-5671.2021.03.04

• 基础研究 • 上一篇    下一篇

一种卵巢癌类器官的制备方法建立及鉴定

  

  1. 广西医科大学附属肿瘤医院实验研究部；广西区域高发肿瘤研究教育部重点实验室
  • 出版日期:2021-06-25 发布日期:2021-07-07
  • 通讯作者: 王琪， E-mail：wangqi@stu.gxmu.edu.cn
  • 基金资助:
    国家自然科学基金项目（81860459）；广西重点研发计划项目（桂科AB1850003）；广西自然科学基金项目（2018GXNSFAA138060）；广西创新驱动发展专项（桂科AA18242040）；区域性高发肿瘤早期防治研究教育部重点实验室自主研究项目（GKE-ZZ202016）

Establishment and identification of a preparation method for ovarian cancer organoids

  • Online:2021-06-25 Published:2021-07-07

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摘要： 目的 探索卵巢癌类器官模型制备方法。方法 选取2019—2020年于广西医科大学附属肿瘤医院初次手术的3例卵巢癌患者原发灶肿瘤组织，解离成细胞悬液后用基质胶Matrigel包被，培养2周形成单个直径超过100 μm的球状体后进行消化传代，培养至第2代的球状体用石蜡包埋切片，脱色后进行HE染色和免疫组化，并与原组织对比，进行组织学和细胞学评判。结果 构建出1例可传代冻存的卵巢癌类器官，HE染色结果显示类器官与原组织的腺癌结构高度相似，免疫组化结果显示原组织与类器官p53、PAX-8、WT-1和CK7标志物的表达情况基本一致。结论 用Matrigel包被并浸润培养液培养的方法可以成功构建与原组织在组织和细胞层面鉴定结果基本一致的卵巢癌类器官。

关键词: 卵巢癌, 类器官, 制备方法, 病理鉴定

Abstract: Objective  To explore and identify the preparation methods of ovarian cancer organoid model. Methods The primary focal tumor tissue of 3 ovarian cancer patients undergoing surgery in Guangxi Medical University Cancer Hospital from 2019 to 2020 were selected, disintegrate into cell suspension and coated with Matrigel, then cultured for 2 weeks to form a single diameter of over 100 μm globule to digested and passed, the globule were embedded in paraffin and slices when cultured to the second generation. After decolorization, the HE staining and immunohistochemistry were performed and compared with the original tissue for histological and cytological evaluation. Results The transpassable cryopreservation organoid was constructed. The results of HE staining showed that the organoid was highly similar to the original tissue in adenocarcinoma structure. The results of immunohistochemistry showed that the expression of p53, PAX-8, WT-1 and CK7 markers were basically the same between the original tissue and the organoid. Conclusions Ovarian cancer organoids with Matrigel coated and infiltration medium can be successfully constructed the ovarian cancer organoids that basically consistent with the original tissue in tissue and cell level identification results.

Key words: Ovarian cancer, Organoid, Preparation method, Pathological identification

中图分类号: 

  • R737.3