微信公众号

官网二维码
中国癌症防治杂志

中国癌症防治杂志 ›› 2021, Vol. 13 ›› Issue (4): 389-394.doi: 10.3969/j.issn.1674-5671.2021.04.11

• 基础研究 • 上一篇    下一篇

LncRNA SNHG20通过miR-520f-3p调控胆管癌细胞增殖和侵袭

  

  1. 哈尔滨医科大学附属第二医院普通外科
  • 出版日期:2021-08-25 发布日期:2021-09-17
  • 通讯作者: 姜兴明 E-mail：xmjiang@hrbmu.edu.cn
  • 基金资助:
    国家自然科学基金项目（81602088）

LncRNA SNHG20 regulates proliferation and invasion of cholangiocarcinoma through miR⁃520f⁃3p

  • Online:2021-08-25 Published:2021-09-17

PDF (PC)

146

赞

摘要： 目的 探讨lncRNA SNHG20和miR-520f-3p对胆管癌细胞增殖和侵袭的影响及其潜在的作用机制。方法 收集2012年3月至2014年3月在哈尔滨医科大学附属第二医院手术切除的20例胆管癌患者肿瘤组织及相应癌旁组织，利用脂质体转染技术分别将si-SNHG20、miR-520f-3p mimics和miR-520f-3p inhibitor及其对照质粒转染至胆管癌CCLP-1细胞，构建过表达和沉默细胞模型。利用qRT-PCR分别检测胆管癌组织和相应癌旁正常组织，以及胆管癌细胞系CCLP-1、QBC939、RBE、TFK-1和正常胆管上皮细胞系HIBEC中SNHG20与miR-520f-3p的表达水平。采用CCK-8法检测细胞增殖能力，划痕和Transwell实验分别检测细胞迁移和侵袭能力。双荧光素酶报告基因实验验证SNHG20与miR-520f-3p的靶向关系。 结果 分别与癌旁组织和正常胆管上皮细胞系HIBEC比较，SNHG20在胆管癌组织和胆管癌细胞系CCLP-1、QBC939、RBE、TFK-1中的表达升高（P<0.05），而miR-520f-3p表达降低（P<0.05）。与si-NC组比较，敲低SNHG20后CCLP-1细胞的增殖、迁移和侵袭能力降低（P<0.05）。SNHG20可以靶向作用并调控miR-520f-3p的表达，敲低 miR-520f-3p能逆转下调SNHG20对CCLP-1细胞增殖和侵袭的抑制作用（t=10.533，P=0.002；t=8.683，P=0.037）。 结论 LncRNA SNHG20能靶向作用于miR-520f-3p，进而调控胆管癌细胞增殖和侵袭。

关键词: 胆管癌, 长链非编码RNA, SNHG20, miR-520f-3p, 增殖, 迁移, 侵袭

Abstract:  Objective  To investigate the effects of lncRNA SNHG20 and miR-520f-3p on the proliferation, migration and invasion of cholangiocarcinoma cells and the potential mechanisms. Methods The tumor tissues and corresponding adjacent tissues of 20 patients with cholangiocarcinoma who were surgically resected in the 2nd Affiliated Hospital of Harbin Medical University from March 2012 to March 2014 were collected. The si-SNHG20, miR-520f-3p mimics and miR-520f-3p inhibitor and their corresponding control plasmids were transfected into cholangiocarcinoma cells CCLP-1 by liposome transfection technology, respectively,  to construct overexpression and silence cell models. The qRT-PCR was used to detect the expression levels of SNHG20 and miR-520f-3p in cholangiocarcinoma tissues and corresponding normal tissues as well as cholangiocarcinoma cells CCLP-1, QBC939, RBE, TFK-1 and normal bile duct epithelial cells HIBEC. The cell proliferation ability was detected by CCK-8 assay. The cell migration and invasion ability were detected by wound healing and Transwell assay, respectively. The targeting relationship between SNHG20 and miR-520f-3p was verified by the dual luciferase reporter gene experiment. Results Compared with the adjacent tissues and normal bile duct epithelial cells HIBEC, the expressions of SNHG20 in CCLP-1, QBC939, RBE, TFK-1 of cholangiocarcinoma cells were significantly higher (P<0.05), while the expressions of miR-520f-3p were decreased (P<0.05). Compared with si-NC group, the proliferation, migration and invasion of CCLP-1 cells were decreased after SNHG20 knockdown (P<0.05). SNHG20 could target and regulate the expression of miR-520f-3p, and the knockdown of miR-520f-3p could reverse the inhibitory effect of down-regulated SNHG20 on the proliferation and invasion of CCLP-1 cells (t=10.533, P=0.002; t=8.683, P=0.037). Conclusions SNHG20 can target miR-520f-3p to regulate the proliferation and invasion of cholangiocarcinoma cells.

Key words: Cholangiocarcinoma, Long non-coding RNA, SNHG20, miR-520f-3p, Proliferation, Migration, Invasion

中图分类号: 

  • R735.8