关键词: 大鼠, 阿霉素, 心肌肌钙蛋白I, 肌酸激酶, 肌酸激酶同功酶, 心肌超微结构, 心肌损伤

Abstract: Objective To investigate the degree and characteristic of myocardial injury on peri-anesthesia wistar rats induced by Adriamycin(ADM) chemotherapy,through analysing the changes of serum cardiac troponin I(cTnI) level,creatine kinase(CK),isoenzyme of creatine kinase(CKMB) activites and myocardial ultrastructure.Methods Thirty-two wistar rats were randomized into four groups:A,B,C and D.Rats in group A and B were treated with ADM by intraperitoneal injection(ip),and rats in group C and D were treated with sodium chloride solution.All rats were treated with anesthesia on the third day after completing the last injection.Anesthesia treatment for the rats in group A and C were opened isoflurane inhalation,while for the rats in group B and D were sodium chloride pentobarbital solution by ip.Blood samples from all animal were taken at pre-anesthesia and 20min post-anaesthesia respectively.The hisptopathological changes of myocardium were observed with light microscope and transmission electron microscope.Results ①The differences of serum cTnI level among the four groups were not statistically significant at pre-anesthesia,but were statistically significant between group A and B,group B and D at post-anesthesia(P<0.01).The cTnI levels in all of the four groups were higher at post-anesthesia than that at pre-anesthesia,with the statistical significance in Group B(P<0.05).②The levels of serum CK and CKMB between pre-anesthesia and post-anesthesia were not statistically significant in four groups(P>0.05).③ With light and electron transmission microscope,pathological changes of myocardium were significant between chemotherapy groups and control groups,as the severe cell degeneration and necrosis were observed in chemotherapy group.The histopathological score of myocardium was significantly different among the four groups.Conclusions Serum cTnI level is a sensitive marker of cardiac injury at peri-anesthesia,and it is more sensitive and specific than CK and CKMB levels.Serum cTnI level also is an important marker to evaluate aggravation of cardiac injury induced by anesthesia on ADM chemotherapy.Inhalation isoflurane have no significant effect on aggravating cardiac injury induced by ADM chemotherapy comparied with sodium chloride pentobarbital,but provides certain degree of myocardial-protection effect.

Key words: Rats, Adriamycin, Cardiac troponin I, Creatine kinase, Isoenzyme of creatine kinase, Myocardial ultrastructure, Myoardial injury