缺氧诱导因子-1α基因多态性与鼻咽癌的关系

中国癌症防治杂志

缺氧诱导因子-1α基因多态性与鼻咽癌的关系

张勇;于斌;

广西医科大学第一附属医院放疗科;

收稿日期:2009-09-25 修回日期:2009-09-25 出版日期:2009-09-25 发布日期:2009-09-25

Correlation of hypoxia inducible factor 1-α polymorphism and nasopharyngeal carcinoma

ZHANG Yong,YU Bin

(Department of Radiotherapy,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China

Received:2009-09-25 Revised:2009-09-25 Online:2009-09-25 Published:2009-09-25

摘要/Abstract

摘要： 目的检测缺氧诱导因子-1α(HIF-1α)C2028T多态性在鼻咽癌中的分布,初步探讨其在鼻咽癌发生发展中的作用和意义。方法采用PCR-RFLP法对90例鼻咽癌患者外周全血中HIF-1αC2028T多态性进行检测,分析与病理分型、UICC分期、T分期、N分期的关系。结果①发现了C/C和C/T两种基因型,HIF-1α基因C2028T多态性分布为C/C纯合子频率87.8%(79/90),C/T杂合子频率12.2%(11/90),C等位基因频率93.9%(169/180),T等位基因频率6.1%(11/180)。②鼻咽癌中HIF-1α因子C2028T基因型频率已达到遗传平衡,具有群体代表性(P>0.05)。③HIF-1αC/T基因型频率与N分期呈正相关(rs=0.39,P=0.00),与UICC分期可能存在正相关(rs=0.21,P=0.05),与病理分型(P=0.98)及T分期(P=0.88)无相关性。结论HIF-1αC/T基因型可能促进了鼻咽癌的局部进展及淋巴结转移,其确切机制有待进一步研究。

关键词: 缺氧诱导因子-1α, 单核苷酸多态性, 鼻咽癌, PCR-RFLP

Abstract: Objective To investigate the distribution of hypoxia inducible factor 1α(HIF-1α) C2028T polymorphism in patients with nasopharyngeal carcinoma(NPC) and its role in NPC carcinogenesis.Methods DNA genome from 90 patients with NPC were extracted,and the HIF-1α C2028T polymorphism was detected by PCR-RFLP method.The relations of HIF-1α C2028T polymorphism with the patients' clinicopathologic features were statically analyzed.Results There were only two genotypes,C/C and C/T.The proportions of genotype C/C and C/T were 87.8%(79/90)and 12.2%(11/90)respectively.The HIF-1α C2028T polymorphism was positively related to patients' N stage(rs=0.39,P=0.00),and was likely having a positive correlation with UICC stage(rs=0.21,P=0.05).There was no correlation between the polymorphism and pathological type(P=0.98) or T stage(P=0.88).Conclusions The genotype C/T of HIF-1α might improve the progress of NPC carcinogenesis,especially might improve the metastasis of NPC.Its exact mechanism deserves further study.

Key words: Hypoxia inducible factor 1α, Single nucleotide polymorphism, Nasopharyngeal carcinoma, PCR-RFLP

张勇;于斌;. 缺氧诱导因子-1α基因多态性与鼻咽癌的关系[J]. 中国癌症防治杂志, 2009, 1(03): 200-202.

ZHANG Yong,YU Bin. Correlation of hypoxia inducible factor 1-α polymorphism and nasopharyngeal carcinoma[J]. Chinese Journal of Oncology Prevention and Treatment, 2009, 1(03): 200-202.

参考文献

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