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中国癌症防治杂志

中国癌症防治杂志 ›› 2018, Vol. 10 ›› Issue (1): 14-18.doi: 10.3969/j.issn.1674-5671.2018.01.04

• 基础研究 • 上一篇    下一篇

牛磺酸对7，12-二甲基苯蒽诱发大鼠乳腺癌的干预作用及其机制

  

  1. 广西医科大学公共卫生学院营养与食品卫生学教研室
  • 出版日期:2018-02-25 发布日期:2018-03-23
  • 通讯作者: 郭松超 2433164518@qq.com
  • 基金资助:

    广西科技基础条件平台建设资助项目（14-91-02）

Effects of taurine on 7，12-dimethyl benzene ［a］ anthracene-induced breast cancer in rats and its mechanism of action

  • Online:2018-02-25 Published:2018-03-23

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摘要：

目的 研究牛磺酸对7，12-二甲基苯蒽（7，12- dimethyl benzene［a］ anthracene，DMBA）诱发大鼠乳腺癌的干预作用及其机制。方法 将30只6周龄雌性SD大鼠随机分为牛磺酸干预组、模型对照组和空白对照组，每组10只。牛磺酸干预组和模型对照组大鼠给予15 mg/100g的DMBA，在牛磺酸干预组的日常饮水中添加3%牛磺酸，一次性灌胃；空白对照组大鼠予1 mL/100 g花生油灌胃。所有大鼠均自由饮水和进食。观察、记录大鼠乳腺肿瘤发生情况，采用酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）检测大鼠血清IL-6和TNF-α含量。结果 与模型对照组相比，牛磺酸干预组肿瘤发生的潜伏期延长，致瘤率、荷瘤总数、血清IL-6和TNF-α水平均下降，差异有统计学意义（P<0.05），肿瘤体积和重量增加，但差异无统计学意义（P>0.05）；与空白对照组相比，模型对照组血清IL-6和TNF-α水平显著升高［（41.41±10.10）pg/mL vs （20.00±10.10） pg/mL，P<0.05；（71.72±25.83） pg/mL vs （42.00±16.69） pg/mL，P<0.05），而牛磺酸干预组升高不明显［（22.48±10.38） pg/mL vs（20.00±10.10） pg/mL，P>0.05；（42.52±9.15） pg/mL vs （42.00±16.69）pg/mL，P>0.05）。 结论 牛磺酸对DMBA诱发大鼠乳腺癌的发生有较明显的抑制作用，其机制可能通过调节机体免疫反应途径实现。

关键词: 乳腺肿瘤, 牛磺酸, 7, 12-二甲基苯蒽, 大鼠, 致瘤率, IL-6, TNF-&alpha

Abstract:

Objective  To study the effects of taurine on 7，12-dimethyl benzene ［a］ anthracene（DMBA）-induced breast cancer in rats and its mechanism of action. Methods A total of 30 male Sprague-Dawley rats were randomly divided into three groups： taurine intervention group，model control group and blank control group. Rats in taurine intervention group  and model control group  were given DMBA （15 mg/100 g） by one-time gavage，while rats in blank control group were given peanut oil （1 mL/100 g）. Animals in taurine intervention group  also received 3% taurine in their drinking water. All rats could drink water and eat ad libitum. Levels of IL-6 and TNF-α in serum were measured using enzyme-linked immunosorbent assay （ELISA）. Results Compared with model control group ，taurine intervention group  showed a lower rate of tumorigenesis，longer tumor latency，and smaller total number of tumors（P<0.05）. Tumor volume and mouse body weight were similar between taurine intervention group and model control group（P＞0.05）. Levels of serum IL-6 and TNF-α were significantly lower in taurine intervention group  than in model control group（P＜0.05），significantly higher in model control group than in blank control group［（41.41±10.10）pg/mL vs （20.00±10.10） pg/mL，P＜0.05 ；（71.72±25.83） pg/mL vs （42.00±16.69） pg/mL，P＜0.05）］，and similar between taurine intervention group and blank control group［（22.48±10.38）pg/mL vs（20.00±10.10） pg/mL，P＞0.05；（42.52±9.15） pg/mL vs（42.00±16.69） pg/mL，P＞0.05）］. Conclusions Taurine has a significant inhibitory effect on the development of DMBA-induced breast cancer in rats. Taurine may exert these effects by regulating the body's inflammatory and immune responses.

Key words: Breast neoplasms, Taurine, 7, 12-dimethyl benzene［a］anthracene, Rat, Tumorigenic rate, IL-6, TNF-α