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中国癌症防治杂志

中国癌症防治杂志 ›› 2011, Vol. 3 ›› Issue (4): 280-283.doi: 10.3969/j.issn.1674-5671.2011.04.03

• 基础研究 • 上一篇    下一篇

不同浓度二磷酸氯喹和雷帕霉素对CNE-2细胞自噬的影响

潘闻燕, 朱小东, 赵伟, 曲颂, 李龄, 苏芳, 李小宇   

  1. 广西医科大学附属肿瘤医院放疗科；△广西医科大学研究生学院
  • 出版日期:2011-12-25 发布日期:2012-01-20
  • 通讯作者: 朱小东 E-mail:zhuxiaodong1966 @yahoo.com.cn。

The effects of chloroquine diphosphate and rapamycin at different concentration on autophagy of CNE-2 cells

PAN Wen-Yan, ZHU Xiao-Dong, ZHAO Wei, QU Song, LI Ling, SU Fang, LI Xiao-Yu   

  • Online:2011-12-25 Published:2012-01-20

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14

摘要： 目的 探讨不同浓度二磷酸氯喹和雷帕霉素对人鼻咽低分化鳞癌(CNE-2)细胞自噬的影响。方法 利用Western-blot技术检测CNE-2细胞经不同浓度二磷酸氯喹(chloroquine disphosphate，CDP)和雷帕霉素(rapamycin)处理后自噬相关蛋白LC3-II和p62的表达情况。以碘化丙啶(propidineiodide,PI)染色技术分别检测CNE-2细胞经抑制自噬最明显的CDP及rapamycin浓度处理后的细胞凋亡率。结果 Western-blot结果示，CNE-2细胞经不同浓度CDP和rapamycin处理后LC3-II和p62蛋白均有不同程度表达。CDP处理组中20.60mg/L浓度组LC3-II和p62表达水平均明显高于其他各浓度组(F1=719.388，F2=73.885,P均＜0.01)；rapamycin处理组中18.28μg/L浓度组LC3-II表达水平明显高于其他各浓度组(F=375.120，P＜0.01)，而p62表达水平却明显低于其他浓度组(F=70.770，P＜0.01)。PI染色检测结果显示，20.60mg/L CDP组及18.28μg/L rapamycin组的细胞凋亡率与对照组间无明显差异(F=0.371，P=0.705)。结论 二磷酸氯喹可以抑制CNE-2细胞的自噬功能，且在20.60mg/L浓度时抑制功能最显著，而不引起细胞凋亡；雷帕霉素可以诱导CNE-2细胞发生自噬，且在18.28μg/L浓度时自噬现象最为明显，而不影响细胞增殖。

关键词: 二磷酸氯喹, 雷帕霉素, 自噬, CNE-2细胞

Abstract: Objective To investigate the effects of chloroquine diphosphate (CDP) and rapamycin at different concentration on autophagy of CNE-2 cells. Methods Western-blot were used to detect the expression level of autophagy-related protein LC3-II (microtubule-associated protein 1 light chain 3，MAP1-LC3-II) and p62 in nasopharyngeal carcinoma cell line CNE-2 that were treated with CDP and rapamycin at different concentrations.Propidium iodide (PI) staining was used to detect the apoptosis rate of CNE-2 cells.Results Among the CDP-treated groups，the expression levels of LC3-II and p62 increased more pronouncedly when CNE-2 cells were treated at the concentration of 20.60mg/L (P＜0.01).Among the rapamycin-treated groups，18.28μg/L group showed increased expression of LC3-II (P＜0.01) and reduced expression of p62 (P＜0.01).The apoptosis rates in 20.60mg/L CDP group and 18.28μg/L rapamycin group were not statistically different from that of the control group (P＞0.05).Conclusions Chloroquine diphosphate can inhibit autophagy of CNE-2 cells at the concentration of 20.60mg/L.Rapamycin can induce autophagy at the concentration of 18.28μg/L.Both of them do not affect the apoptosis rate of CNE-2 cells.

Key words: Chloroquine diphosphate, Rapamycin, Autophagy, CNE-2 cells