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中国癌症防治杂志

中国癌症防治杂志 ›› 2013, Vol. 5 ›› Issue (2): 100-104.doi: 10.3969/j.issn.1674-5671.2013.02.04

• 基础研究 • 上一篇    下一篇

广西壮族人群microRNA-146a基因多态性与肝癌遗传易感性的相关性研究

  

  1. 广西医科大学附属肿瘤医院实验研究部；广西医科大学研究生学院；广西扶绥县人民医院
  • 出版日期:2013-06-25 发布日期:2013-06-27
  • 通讯作者: 张春燕 E-mail:zcy2016@aliyun.com
  • 基金资助:

    国家自然科学基金资助项目（81060173）；广西自然科学基金资助项目（2010GXNSFA013242）；广西医疗卫生重点科研课题（重2010117）；广西研究生教育创新计划资助项目（YCSZ2012046）

Correlation between microRNA-146a polymorphism and primary liver carcinoma in the Guangxi Zhuang population

  • Online:2013-06-25 Published:2013-06-27

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793

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被引次数

13

摘要： 目的 探讨广西壮族人群microRNA-146a基因rs 2910164位点多态性与肝癌遗传易感性的关系。方法 应用病例-对照研究方法，分别选择广西肝癌高发区壮族肝癌患者110例以及健康对照者110名，采用基质辅助激光解吸附电离飞行时间质谱技术（MALDI-TOF-MS）对microRNA-146a基因rs 2910164位点进行SNP分型，以χ2检验比较microRNA-146a基因rs 2910164位点基因型在病例组与对照组之间的分布差异，采用非条件Logistic回归分析其多态基因型与肝癌发生危险性的关系。结果 病例组中microRNA-146a基因rs 2910164位点的CG基因型分布频率高于对照组，差异有统计学意义（P=0.026）。Logistic回归分析结果显示，携带CG基因型者患肝癌风险增高（OR=3.473，95%CI：1.116～10.802，P=0.032），GG基因型在两组间分布频率的差异无统计学意义（P>0.05）。其等位基因C和G在病例组中的分布频率分别为62.7%和37.3%，在对照组中的分布频率分别为67.7%和32.3%，等位基因C或G在两组间分布频率的差异均无统计学意义（P>0.05）。结论 microRNA-146a基因rs 2910164位点的CG基因型携带者的肝癌发病风险显著增加，其CG基因型可能是广西壮族人群肝癌发病的遗传易感因素之一。

关键词: 肝肿瘤, microRNA-146a, rs 2910164, 单核苷酸多态性

Abstract: Objective To investigate the correlation between the microRNA-146a rs 2910164 polymorphism and primary liver carcinoma（PLC） in a Guangxi Zhuang population． Methods Genotypes and alleles of rs 2910164 were determined in 110 PLC patients and 110 healthy controls in a Guangxi area with high PLC incidence using matrix-assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF MS）. Differences in the distribution of polymorphisms between patients and controls were analyzed by the chi-squared test.Correlation between the rs 2910164 polymorphism and PLC risk was evaluated by non-conditional logistic regression. Results The CG genotype was significantly more frequent in PLC patients than in controls（P=0.026），and it was associated with increased risk of PLC（OR=3.473，95%CI 1.116-10.802，P=0.032）．However，the frequency of the GG genotype was similar between the two groups（P>0.05）．Similarly，the frequencies of the C and G alleles were similar in patients（62.7% and 37.3%，respectively） and controls（67.7% and 32.3%，P>0.05）． Conclusions Carriers of the microRNA-146a rs 2910164 CG genotype are at elevated risk of PLC.This genotype may be a PLC risk factor in the Guangxi Zhuang population．

Key words: Liver neoplasms, microRNA-146a, rs 2910164, Single nucleotide polymorphisms