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中国癌症防治杂志

中国癌症防治杂志 ›› 2014, Vol. 6 ›› Issue (3): 230-234.doi: 10.3969/j.issn.1674-5671.2014.03.03

• 基础研究 • 上一篇    下一篇

雷公藤红素对裸鼠肝癌皮下移植瘤抑制作用的研究

  

  1. 江苏南通大学比较医学研究所
  • 出版日期:2014-09-25 发布日期:2014-10-17
  • 通讯作者: 邵义祥 E-mail:shaoyx@ntu.edu.cn
  • 基金资助:

    江苏省高校自然科学研究面上项目（13KJB310015）；南通市科技计划项目（BK2013045）；南通大学引进人才科研启动基金资助项目（13R30）；南通大学自然科学科研基金助项目（13ZY008）

Celastrol-mediated growth inhibition of subcutaneously transplanted hepatocarcinoma in nude mice

  • Online:2014-09-25 Published:2014-10-17

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摘要： 目的 研究天然活性化合物雷公藤红素（celastrol）对裸鼠移植肿瘤的抑制作用。方法 建立人源肝癌HepG2细胞的裸鼠皮下移植肿瘤模型。20只裸鼠随机分为4组，即空白对照组和雷公藤红素高剂量组、中剂量组和低剂量组，每组分别按4.0 mg/kg、2.0 mg/kg、1.0 mg/kg雷公藤红素腹腔给药，每日一次，每周5 d，共给药30 d，实验中每5 d测量一次肿瘤体积的变化。结果 雷公藤红素高剂量组、中剂量组和低剂量组的体积抑瘤率分别是89.52%、65.60%和12.21%，与空白对照组相比，高剂量组、中剂量组的差异具有统计学意义（P＜0.05）。高剂量雷公藤红素给药后对裸鼠肝脏和肾脏等产生严重的毒性反应。结论 雷公藤红素对裸鼠肝癌皮下移植瘤具有良好的体内抗肿瘤效果，应进一步开展药物新剂型及分子结构优化方面的研究，减少其毒副反应。

关键词: 肝肿瘤, 雷公藤红素, 肝癌皮下移植瘤模型, 抑制, 毒性

Abstract:  Objective To study the potential of celastrol to inhibit growth of transplanted tumors in nude mice. Methods Nude mice（n=20） subcutaneously injected with HepG2 cells were randomly divided into 4 groups and treated by abdominal injection with 0，1.0，2.0 and 4.0 mg/kg once daily five times per week. Tumor size was measured every five days. Results Tumor volume inhibition was 12.21% for 1.0 mg/kg，65.60% for 2.0 mg/kg and 89.52% for 4.0 mg/kg，and tumor volume was significantly smaller in animals treated with 2.0 or 4.0 mg/kg than in control animals. Severe liver and kidney toxicity were observed in animals treated with 4.0 mg/kg. Conclusion Celastrol shows good anticancer activity in this mouse model of liver cancer. Studies are needed to optimize dosing and drug structure to reduce toxicity.

Key words: Liver neoplasm, Celastrol, Liver cancer model, Inhibition, Toxicity