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中国癌症防治杂志

中国癌症防治杂志 ›› 2017, Vol. 9 ›› Issue (1): 45-49.doi: 10.3969/j.issn.1674-5671.2017.01.09

• 基础研究 • 上一篇    下一篇

CD166对鼻咽癌CNE-2细胞放射敏感性的影响

  

  1. 广西医科大学附属肿瘤医院放疗科 广西壮族自治区肿瘤防治研究所；广西医科大学研究生院；广西区域性高发肿瘤早期防治研究重点实验室；区域性高发肿瘤早期防治研究教育部重点实验室
  • 出版日期:2017-02-25 发布日期:2017-03-24
  • 通讯作者: 朱小东。E-mail: zhuxiaodong@gxmu.edu.cn
  • 基金资助:

      国家自然科学基金资助项目(81360343);区域性高发肿瘤早期防治研究教育部重点实验室自主研究资助项目（GKE2015-ZZ09）

Effects of CD166 on radioresensitivity of nasopharyngeal carcinoma cell line CNE-2

  1.  Department of Radiation Oncology，Affiliated Tumor Hospital of Guangxi Medical University/ Guangxi Cancer Prevention and Treatment Research Institute； Graduate School of Guangxi Medical University；Guangxi Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor，Guangxi Medical University；4Key Laboratory of High-Incidence-Tumor Early Prevention and Treatment，Ministry of Education
  • Online:2017-02-25 Published:2017-03-24
  • Contact: Zhu Xiaodong. E-mail: zhuxiaodong@gxmu.edu.cn

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摘要：

目的 探讨CD166对鼻咽癌CNE-2细胞放射敏感性的影响。方法 采用流式细胞术检测鼻咽癌CNE-2细胞膜CD166阳性表达率，免疫磁珠分选技术获得CNE-2-CD166（＋）、CNE-2-CD166（－）细胞亚群，克隆形成实验验证其放射敏感性，CCK-8法及流式细胞术检测10 Gy剂量照射前后的细胞增殖率及细胞凋亡率。结果 CNE-2细胞膜CD166阳性表达率为（24.27±5.31，分选后CNE-2-CD166（＋）细胞CD166阳性表达率为96.5%，CNE-2-CD166（－）细胞为0.6%。克隆形成实验结果显示，CNE-2-CD166（－）细胞相对CNE-2-CD166（＋）细胞的放射增敏比（sensitization enhancement ratio,SER）为1.24。10 Gy照射后24 h、48 h和72 h，CNE-2-CD166（＋）细胞的存活分数分别为（(76.66±3.36)%、(62.44±1.66)%和(55.21±3.39)，CNE-2-CD166（－）细胞为(60.34±5.13)%、(41.11±3.00)%和(35.28±4.36)%，同时段内两组细胞的存活分数比较，差异均有统计学意义（P＜0.05）。10 Gy照射前，CNE-2-CD166（＋）细胞和CNE-2-CD166（－）细胞凋亡率分别为(5.87±1.14)%和(6.67±1.68)%，差异无统计学意义（P＞0.05）；10 Gy照射后48 h，CNE-2-CD166（＋）细胞凋亡率为（(19.37±2.54)%，显著低于CNE-2-CD166（－）细胞的(28.90±4.04)%，差异有统计学意义（P=0.026）。结论 CD166表达阳性的鼻咽癌CNE-2细胞对放射更具抗拒性，可能与减少细胞受照射后的凋亡率降低有关。)%
 
 

关键词: 鼻咽肿瘤, 放射敏感性, CD166, CNE-2细胞, 生物学行为

Abstract:

Objective The present study aimed to investigate the effect of CD166 on the radiosensitivity of nasopharyngeal carcinoma cell line CNE-2. Methods CD166 expression in the CNE-2 cell membrane was detected by flow cytometry. CNE-2-CD166（+） and CNE-2-CD166（-） cell lines were obtained using magnetic-activated cell sorting technology. Radiosensitivity，cell proliferation，and apoptosis were assessed，respectively，using a colony formation assay，cell counting kit-8 assay and flow cytometry. Results The CD166（+） rate in the CNE-2 cell membrane was（24.27±5.31）%；after immunomagnetic separation，the CD166（+） rate was 96.5% among CNE2-CD166（+） cells and 0.6% among CNE2-CD166（-） cells. The radiation sensitivity ratio was 1.24. After 10-Gy irradiation，The CCK-8 assay indicated that the survival fraction of CNE-2-CD166（+） cells was （76.66±3.36）% in 24 h，（62.44±1.66）% in 48 h and （55.21±3.39）%in 72 h，significantly higher than that of CNE-2-CD166（-） cells:（60.34±5.13）% in 24 h、（41.11±3.00）% in 48 h，（35.28±4.36）% in 72 h（P＜0.05）. After 10-Gy irradiation，the apoptotic rate of CNE-2-CD166（+） cells was significantly lower than that of CNE-2-CD166（-）cells（P=0.026）. Conclusion Our data suggest that CD166（+） nasopharyngeal carcinoma CNE-2 cells are more resistant to radiation，and the mechanism may be associated with reduced apoptosis rate after irradiation.

Key words: Nasopharyngeal neoplasms, Radiosensitivity, CD166, CNE-2 cell, Biological behavior