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中国癌症防治杂志

中国癌症防治杂志 ›› 2020, Vol. 12 ›› Issue (4): 403-408.doi: 10.3969/j.issn.1674-5671.2020.04.07

• 鼻咽癌精准诊疗专栏 • 上一篇    下一篇

miR-6732-3p对鼻咽癌放射抗拒细胞株CNE-2R放射敏感性的影响

  

  1. 广西医科大学附属肿瘤医院放疗科；广西鼻咽癌临床医学研究中心
  • 出版日期:2020-08-25 发布日期:2020-08-31
  • 通讯作者: 曲颂 E-mail：daisyqs2002@163.com
  • 基金资助:

    广西自然科学基金项目（2018GXNSFAA281032）；广西医疗卫生适宜技术开发与推广应用项目（S2018038）；广西研究生教育

    创新计划项目（YCSW2019120）；区域性高发肿瘤早期防治研究教育部重点实验室（广西医科大学）自主研究项目（GK2018-08；GKE2019-15

    ）

Effect of miR-6732-3p expression on radio-sensitivity of radio-resistant nasopharyngeal carcinoma cell line CNE-2R

  • Online:2020-08-25 Published:2020-08-31

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摘要：

目的 探讨miR-6732-3p表达对人鼻咽癌CNE-2R细胞放射敏感性的影响。方法 采用RT-qPCR检测miR-6732-3p在CNE-2和CNE-2R细胞株中的表达。用miR-6732-3p慢病毒转染CNE-2R细胞后，将CNE-2R细胞分为三组，分别为CNE-2R组（正常对照组）、miR-6732-3p NC组（转染对照组）、miR-6732-3p inhibition组（转染组）。RT-qPCR检测各组细胞中miR-6732-3p的表达，CCK-8、流式细胞术和克隆形成实验分别检测转染前后的细胞增殖、凋亡能力及放射敏感性。结果 RT-qPCR检测结果显示，miR-6732-3p在CNE-2R细胞中的表达量明显高于CNE-2细胞（P=0.036）。采用慢病毒转染CNE-2R细胞后，转染组细胞miR-6732-3p 的表达量均较转染对照组和正常对照组低（P＜0.05）。CCK-8法检测结果显示，与两个对照组相比，转染组细胞生长受抑制（P＜0.001）；在不同照射剂量下，转染组细胞的存活分数均明显降低（P＜0.001）。流式细胞仪检测结果显示，未经照射（0 Gy）和接受8 Gy照射后转染组细胞凋亡率均较转染对照组及正常对照组明显升高（P＜0.05）。克隆形成实验结果显示，转染组的放射生物学参数D0、Dq、SF2值均低于两个对照组（P＜0.05）；在不同照射剂量下，转染组细胞存活分数亦低于两个对照组（P＜0.001）。结论 沉默miR-6732-3p可提高鼻咽癌CNE-2R细胞的放射敏感性，为鼻咽癌放射抗拒的分子靶向治疗提供新思路。

关键词: 鼻咽癌, 放射敏感性, miR-6732-3p

Abstract:

Objective To investigate the effect of miR-6732-3p expression on the radio-sensitivity of human nasopharyngeal carcinoma CNE-2R cells. Methods The expression of miR-6732-3p in CNE-2 and CNE-2R cell lines was detected by RT-qPCR. After transfection of CNE-2R cells with miR-6732-3p lentivirus，CNE-2R cells were divided into CNE-2R（normal control group）， miR-6732-3p NC （transfection control group），and miR-6732-3p inhibition（transfection group）. The expression of miR- 6732-3p in each group was detected by RT-qPCR. The proliferation ability，apoptosis ability and radio-sensitivity of cells before and after transfection were detected by CCK-8 assay，flow cytometry and cloning formation assay，respectively. Results The RT-qPCR showed that the expression level of miR-6732-3p in CNE-2R cells was significantly higher than that of CNE-2 cells（P=0.036）. After transfection of CNE-2R cells with lentivirus，the expression level of miR-6732-3p in the transfection group was lower than those in the transfection control group and the normal control group（P＜0.05）. CCK-8 assay results showed that compared with the two control groups，the cell growth of the transfection group was inhibited （P ＜0.001）；the survival fraction （SF） of the transfection group was significantly reduced under different irradiation doses（P＜0.001）. The flow cytometry detection results showed that the apoptotic rate of transfection group after non-irradiation（0 Gy） and 8 Gy irradiation was significantly increased than that of the transfection control group and the normal control group（P＜0.05）. The cloning formation assay indicated that the radiobiological parameters D0，Dq and SF2 in the transfection group were lower than those of the two control groups（P<0.05）；the SF of the transfection group was also lower than the two control groups under different irradiation doses（P＜0.001）. Conclusions Silencing miR-6732-3p expression can significantly improve the radio-sensitivity of nasopharyngeal carcinoma CNE-2R cells，providing a new idea for molecular targeted therapy of radio-resistance in nasopharyngeal carcinoma.

Key words: Nasopharyngeal carcinoma, Radio-sensitivity, miR-6732-3p

中图分类号: 

  • R739.63