关键词: 胶质瘤, miR-205-5p, Runx2, 生物学行为

Abstract: Objective  To investigate the expression of miR-205-5p in glioma and its regulation on the proliferation, migration, invasion of glioma cells and possible mechanisms. Methods The glioma tissue and adjacent tissue specimens from 58 newly diagnosed glioma patients in The First people′s Hospital of Chenzhou from June 2018 to December 2019 were collected. U251 and T98G cells were transfected with miR-205-5p mimic to construct the overexpressed cell models. The expression level of miR-205-5p in glioma tissues and cell models were detected by RT-qPCR, and the relationship between the expression level and the clinicopathological characteristics of the patients was analyzed. The proliferation, clonal formation, migration and invasion of glioma cells were detected by CCK-8, plate clonal formation assay, scratch test and Transwell assay, respectively. The influence of overexpression of miR-205-5p on Runx2 protein expression level was detected by Western blot. The targeted relationship between miR-205-5p and Runx2 was detected by double luciferase reporter assay. Results miR-205-5p was low expressed in glioma tissues and glioma cells U251 and T98G(all P<0.01), and its expression level was related to the tumor size and pathological grade(both P＜0.05). Overexpression of miR-205-5p could significantly inhibit the proliferation, clonal formation, migration and invasion of glioma cells(all P＜0.05). Double luciferase reporter assay confirmed that Runx2 was a potential target gene of miR-205-5p. Overexpression of miR-205-5p significantly down-regulated Runx2 protein expression in glioma cells(P＜0.001). Conclusions miR-205-5p is low expressed in glioma. Overexpression of miR-205-5p can inhibit the proliferation, clonal formation, migration and invasion of glioma cells, and its mechanism may be related to the targeted regulation of Runx2. 

Key words:  , Glioma, miR-205-5p, Runx2, Biological behaviour