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中国癌症防治杂志

中国癌症防治杂志 ›› 2016, Vol. 8 ›› Issue (2): 88-91.doi: 10.3969/j.issn.1674-5671.2016.02.04

• 临床研究 • 上一篇    下一篇

人脑胶质瘤中OIP5和CTCFL的表达及临床意义

  

  1. 广西医科大学基础医学院组织学与胚胎学教研室；广西高校基础医学重点实验室；广西医科大学第一附属医院神经外科
  • 出版日期:2016-04-25 发布日期:2016-05-13
  • 通讯作者: 谢小薰 xiaoxunxie@gmail.com
  • 基金资助:

    国家自然科学基金资助项目（81360374，81460382，81560408）； 广西自然科学基金资助项目（2012GXNSFBA053107）；广西医科大学青年科学基金资助项目（GXMUYSF2014011，GXMUYSF201409）

Expression and clinical significance of OIP5 and CTCFL in human glioma

  • Online:2016-04-25 Published:2016-05-13

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摘要：

目的 检测癌-睾丸抗原（cancer-testis antigen，CTA） OIP5和CTCFL mRNA在人脑胶质瘤（以下简称胶质瘤）中的表达，探讨OIP5和CTCFL作为胶质瘤免疫治疗靶抗原的可能性。方法 用逆转录-聚合酶链反应（reverse transcription polymerase chain reaction，RT-PCR）方法，检测92例胶质瘤组织中OIP5和CTCFL mRNA的表达水平；分析其表达与胶质瘤患者临床病理特征的相关性。结果 在92例胶质瘤组织中，OIP5和CTCFL mRNA的阳性表达率分别为18.47％（17/92）和20.65%（19/92）；DNA 测序结果证实 RT-PCR产物为OIP5和CTCFL基因cDNA的扩增片段。OIP5和CTCFL mRNA的表达与胶质瘤患者的性别、年龄、病理分级、组织学类型和卡氏评分等临床指标均无关 （P>0.05）。结论 OIP5和CTCFL mRNA在胶质瘤组织中的阳性表达率不高，能否作为胶质瘤免疫治疗的新靶点有待进一步评估。

关键词: 胶质瘤, 癌-睾丸抗原, OIP5, CTCFL, 肿瘤免疫治疗

Abstract:

Objective To measure mRNA levels of the CTAs OIP5 and CTCFL in glioma patients and investigate the suitability of the encoded proteins as targets in glioma immunotherapy. Methods RT-PCR was used to measure levels of OIP5 and CTCFL mRNAs in 92 tissue specimens and explore the correlation of OIP5 and CTCFL expression with clinical indices. Results Expression rates of OIP5 and CTCFL were 18.47％（17/92）and 20.65%（19/92），respectively. DNA sequencing results confirmed that RT-PCR synthesis was successful. Statistical analysis showed that OIP5 and CTCFL expression had no correlation with any clinical indices including sex，age，pathological grading，histological subtypes and Karnofsky performance score（P>0.05）. Conclusion OIP5 and CTCFL are expressed specifically in glioma，but expression rates are low. Thus the feasibility of targeting these proteins as antigens in immunotherapy requires further study.

Key words: Glioma, Cancer-testis antigen, OIP5, CTCFL, Tumor immunotherapy