Abstract: Objective To explore the value of serum microRNA-1，LASP1，TAGLN2 and LGALS3BP in the diagnosis of esophageal cancer. Methods A total of 128 esophageal cancer patients（observation group） and 134 healthy controls （control group） collected from a third-class hospital in Shenyang from January 2012 to January 2016 were enrolled in this study. The protein expression levels of LASP1，TAGLN2，and LGALS3BP in serum were detected by ELISA，and the mRNA expression of microRNA-1 in serum was detected by PCR. The ROC curve was used to evaluate the diagnostic values of serum LASP1，TAGLN2，LGALS3BP and microRNA-1 in esophageal cancer. Results The protein expression levels of LASP1，TAGLN2 and LGALS3BP in the observation group were higher than those in the control group（P<0.001），but the expression level of microRNA-1 was lower than that in the control group（P<0.001）.In esophageal cancer patients，the serum LASP1，TAGLN2，and LGALS3BP were negatively correlated with microRNA-1（P<0.001）.  The AUC of serum microRNA-1，LASP1，TAGLN2 and LGALS3BP in the diagnosis of esophageal cancer were 0.940（95%CI：0.913-0.967），0.890（95%CI：0.843-0.936），0.841（95%CI：0.794-0.889） and 0.924（95%CI：0.893-0.956），and the AUC for combined diagnosis was 0.970（95%CI：0.954-0.987）. Conclusion The expression levels of LASP1，TAGLN2，LGALS3BP，and microRNA-1 in serum have diagnostic value for esophageal cancer. 

Key words: Esophageal cancer, microRNA-1, LASP1, TAGLN2, LGALS3BP