Abstract: Objective To investigate the distribution of hepatitis B virus（HBV） S gene mutation in hepatocellular carcinoma （HCC） and pericarcinoma liver tissues of Guangxi. Methods Totals of 43 patients with HBV-related HCC who underwent HCC surgery in the Guangxi Medical University Cancer Hospital，were selected as subjects from 2015 to 2016，and the HCC tissues and pericarci-noma liver tissues were collected. The HBV S gene fragments were amplified by nest PCR and sequenced. The sequence of S gene was analyzed by Chromas and DNAMAN software. Results There was no significant difference in the distribution of hot spot mutation of HBV S gene between HCC tissues and pericarcinoma liver tissues（P>0.05）. The stop mutation of small hepatitis B surface antigen （SHBs） were only found in cancer tissues， which were sC69*，sW74*，sL94*，sW172* and sW182*. Conclusions For HBV-related HCC in Guangxi，there is no specific distribution of hot spot mutations in HBV S gene  between cancer tissues and paracan-cerous liver tissues，SHBs stop mutation may affect the occurrence and development of HCC.

Key words: Hepatocellular carcinoma, Hepatitis B virus, S gene, Mutation