Table of Content

25 October 2019, Volume 11 Issue 5 Previous Issue    Next Issue

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ownstaging advanced hepatocellular carcinom：surgical strategy and prospect  XIANG Bangde, LI Lequn 2019, 11 (5):  359-362.  doi: 10.3969/j.issn.1674-5671.2019.05.01 Abstract ( 253 )   PDF (499KB) ( 197 )   Save Related Articles | Metrics

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The clinical practice expert consensus of tumor biomarkers in recurrent or metastatic breast cancer（2019） The Expert Board of Hereditary Tumor Biomarkers, the Professional Committee of Tumor Biomarker, Chinese Anti-cancer Association, The Expert Board of Breast Cancer Biomarkers, the Professional Committee of Tumor Biomarker, Chinese Anti-cancer Association 2019, 11 (5):  363-374.  doi: 10.3969/j.issn.1674-5671.2019.05.02 Abstract ( 614 )   PDF (539KB) ( 906 )   Save Related Articles | Metrics

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Effect of lncRNA MAFG-AS1 and miR-143-3p on the proliferation and apoptosis of cervical cancer cells and its mechanism ZHAO Chenyang, LI Qiong 2019, 11 (5):  375-381.  doi: 10.3969/j.issn.1674-5671.2019.05.03 Abstract ( 348 )   PDF (1635KB) ( 259 )   Save Objective To investigate the effects of lncRNA MAFG-AS1 and miR-143-3p on proliferation and apoptosis of cervical cancer cells and its potential mechanism. Methods  After MAFG-AS1 and miR-143-3p expression vectors were transfected into Hela cells，qRT-PCR was used to detect the expression of miR-143-3p and MAFG-AS1 mRNA in Hela cells； protein expression，cell proli-feration activity and apoptosis were detected by Western blot， MTT assay and flow cytometry， respectively；dual-luciferase reporter gene was used to the interaction between lncRNA MAFG-AS1 and miR-143-3p. Results Compared with adjacent normal tissues，MAFG-AS1 mRNA expression was increased in cervical cancer tissues （0.905±0.115 vs 2.835±0.164，t=43.091，P<0.001），and miR-143-3p expression level was decreased （0.944±0.075 vs  0.382±0.071，t=24.336，P<0.001）. Compared with the normal cervical cell line Ect1/E6E7，the expression of MAFG-AS1 mRNA in Hela cells was significantly increased（P<0.001），and the expression of miR-143-3p was significantly decreased（P<0.001）. Down-regulation of MAFG-AS1 and miR-143-3p overexpression inhibited the proliferation of Hela cells and promoted apoptosis， inhibited the expression of Bcl-2 and Cyclin D1 proteins，and promoted the expression of Bax，Caspase-3，p21，and p27 proteins. MAFG-AS1 targeted to regulate the expression of miR-143-3p. Inhibition of miR-143-3p expression reversed the effect of MAFG-AS1 on inhibiting proliferation and promoting apoptosis Hela cells. Conclusions lncRNA MAFG-AS1 can inhibit the proliferation of cervical cancer cells and promote their apoptosis. The mechanism may be related to miR-143-3p，which will provide new targets for the prevention and treatment of cervical cancer. Related Articles | Metrics

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Analysis of HBV S gene mutation in HBV-related hepatocellular carcinoma tissues and pericarcinoma liver tissues in Guangxi LI Hong, WU Linghong, DENG Wei, HUANG Youquan, HAN Xiao, LIAN Shanxiong, HUANG Tianren 2019, 11 (5):  382-386.  doi: 10.3969/j.issn.1674-5671.2019.05.04 Abstract ( 431 )   PDF (916KB) ( 1015 )   Save Objective To investigate the distribution of hepatitis B virus（HBV） S gene mutation in hepatocellular carcinoma （HCC） and pericarcinoma liver tissues of Guangxi. Methods Totals of 43 patients with HBV-related HCC who underwent HCC surgery in the Guangxi Medical University Cancer Hospital，were selected as subjects from 2015 to 2016，and the HCC tissues and pericarci-noma liver tissues were collected. The HBV S gene fragments were amplified by nest PCR and sequenced. The sequence of S gene was analyzed by Chromas and DNAMAN software. Results There was no significant difference in the distribution of hot spot mutation of HBV S gene between HCC tissues and pericarcinoma liver tissues（P>0.05）. The stop mutation of small hepatitis B surface antigen （SHBs） were only found in cancer tissues， which were sC69*，sW74*，sL94*，sW172* and sW182*. Conclusions For HBV-related HCC in Guangxi，there is no specific distribution of hot spot mutations in HBV S gene  between cancer tissues and paracan-cerous liver tissues，SHBs stop mutation may affect the occurrence and development of HCC. Related Articles | Metrics

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Effect of LRP1 on the incidence of prostate cancer based on genome-wide ChinaPca data and gene expression XIONG Han, LIN Rui, TANG Weizhu, MENG Qinggui, ZHANG Qingyun, CHEN Yang, CHENG Jiwen 2019, 11 (5):  387-392.  doi: 10.3969/j.issn.1674-5671.2019.05.05 Abstract ( 342 )   PDF (2208KB) ( 264 )   Save Objective To investigate the effect of low density lipoprotein receptor-related protein-1 （LRP1） gene on prostate cancer （Pca）. Methods Based on ChinaPca GWAS data，nine single nucleotide polymorphism（SNP） of LRP1 were given to verify the prostate cancer susceptibility. Immunohistochemical tests were performed in 12 Pca tissues，18 normal prostate tissues and 4 prostatitis tissues，respectively. LRP1 gene expression data from TCGA and GTEx databases were analyzed，and the interaction network of LRP1 genes on the String website was built. Results ChinaPca GWAS data showed that rs11172113 of LRP1 gene might be the susceptible site of Pca（P=0.083），and TC genotype was related to the risk of Pca（OR=1.267，95%CI：1.066-1.505，P=0.007）. The results of immuno - histochemistry experiment and TCGA database analysis showed that there was no statistically significant difference in the expression of LRP1 in Pca tissues and normal prostate tissues（P>0.05）. After integrating the data in TCGA and GTEx databases，the expression of LRP1 in Pca tissues was decreased（P<0.05）. The interaction network showed that LRP1 was associated with the PLAT，PDGFB and PDGFRB  genes  which related to the prostate cancer pathway. Conclusions rs11172113 of LRP1 may be a genetic predisposition to prostate cancer. The low expression of LRP1 gene in prostate cancer. LRP1 may regulate the prostate cancer-related pathways by regulating the expression of PLAT，PDGFB and PDGFRB genes and affect the incidence of prostate cancer. Related Articles | Metrics

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Prognostic value of tumor burden score in patients with hepatocellular carcinoma WANG Yanyan, LIU Jiaming, WANG Lijun, XU Da, XING Baocai 2019, 11 (5):  393-397.  doi: 10.3969/j.issn.1674-5671.2019.05.06 Abstract ( 591 )   PDF (935KB) ( 450 )   Save Objective To evaluate the prognostic value of tumor burden score（TBS） in patients with hepatocellular carcinoma（HCC）. Methods The clinical data of 487 patients with HCC undergoing liver resection were retrospectively collected. Time-dependent ROC analysis was conducted to evaluate the ability of TBS in predicting overall survival（OS） and disease-free survival （DFS）. Multivariable Cox regression analysis was used to determine independent factors affecting prognosis. Results Time-dependent ROC analysis showed that the AUC of TBS for predicting OS at 5 years was 0.722，higher than that of maximum tumor diameter （0.711） and tumor number（0.548）. TBS stratified patients into 4 prognostic risk strata，namely TBS ≤3 （n=70，14.4%），3n=175，35.9%），5n=150，30.8%），and TBS>8（n=92，18.9%）. The 5-year OS for patients in the 4 risk strata was 87.5%，75.7%，62.9%，and 36.3%，respectively（P<0.001）. The 5-year DFS in the 4 risk strata was 62.4%，44.2%，31.1%，and 12.9%，respectively（P<0.001）. Multivariable analysis also revealed that TBS was an independent factor for both OS and DFS in patients with HCC after liver resection. Conclusion TBS may be a good tool for predicting the prognosis of patients with HCC after liver resection. Related Articles | Metrics

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Surgical treament for T790M-positive lung adenocarcinoma patients with long bone metastases of extremities SU Ning, CHEN Long, Wang Lin, HE Donglei, WANG Cuiying, FENG Jun 2019, 11 (5):  398-402.  doi: 10.3969/j.issn.1674-5671.2019.05.07 Abstract ( 353 )   PDF (816KB) ( 300 )   Save Objective To study and compare the clinical application between surgical treatment and non-surgical treatment in T790M-positive lung adenocarcinoma patients with long bone metastases in extremities. Methods Thirty-six patients with T790M-positive lung adenocarcinoma with long bone metastases who were admitted to Dongfang Hospital of Dongfang City，the Third People's Hospital of Hainan Province，Hainan General Hospital and the First Affiliated Hospital of Hainan Medical College from July 2016 to December 2018 were enrolled. They were divided into the surgical treatment group（n=17） and the non-surgical treatment group（n=19） based on treatment methods. The patients in the surgical treatment group underwent tumor resection and limb functional reconstruction，The non-surgical treatment group received minimally invasive treatment（including interventional therapy） or radiotherapy. The clinical efficacy was compared between the two groups. Results The visual analogue scale（VAS） score reduction rates，the Ennecking score increasing rates，the Kanofsky score increasing rates of patients in surgical treatment group were higher than those in non-surgical treatment group respectively（88.2% vs 52.6%，χ2=5.360，P=0.021；82.4% vs 47.4%，χ2=4.760，P=0.029；70.6% vs 36.8%，χ2=4.100，P=0.043）. There was no significant difference in the response rate and the disease control rate of both groups after treatment（52.9% vs 47.4%，χ2=0.111，P=0.738；76.5% vs 78.9%，χ2=0.032，P=0.858）. The median survival of patients of the surgical treatment group was higher than non-surgical treatment group，the differences was statistically significant （12.0 months vs 9.0 months，χ2=5.009，P=0.024）. Conclusion Surgical treatment can significantly reduce pain，improve limb function and the median survival of T790M-positive lung adenocarcinoma patients with long bone metastases in extremities. Related Articles | Metrics

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The evaluating value of neutrophil to lymphocyte ratio on efficacy and prognosis of concurrent chemo-radiotherapy in elderly patients with advanced cervical squamous cell carcinoma WEI Xiaoqiang, WANG Junjie, TANG Meng 2019, 11 (5):  403-407.  doi: 10.3969/j.issn.1674-5671.2019.05.08 Abstract ( 262 )   PDF (854KB) ( 197 )   Save Objective To investigate the relationship between neutrophils to lymphocytes ratio（NLR） and concurrent chemotherapy（CCRT） and prognosis in elderly patients with advanced cervical squamous cell carcinoma. Methods A retrospective analysis was performed in 103 elderly patients with advanced cervical squamous cell carcinoma（stage Ⅱ B-ⅢB） who underwent CCRT in Qingdao Central Hospital from January 2014 to December 2018. The patients were divided into high NLR group（NLR≥3.48） and low NLR group（NLR＜3.48）. The efficacy of CCRT between the two groups were compared，and the factors affecting prognosis were analyzed. Results The clinical efficacy of the high NLR group was statistically significantly lower than that of the low NLR group（57.69% vs 84.31%，χ2=8.840，P=0.004）. NLR was not related to age，size of tumor and degree of histological differentiation（P>0.05），but related to FIGO stage and lymph node metastasis（P<0.05）. The median OS of the high NLR group was lower than that of the low NLR group （24.0 months vs 46.0 months，χ2=7.187，P=0.007）. Multivariate Cox regression analysis showed that NLR≥3.48 was an independent risk factor for OS in elderly patients with advanced cervical squamous cell carcinoma（HR=1.937，95%CI：1.086-3.456，P=0.025）. Conclusions In elderly patients with advanced cervical squamous cell carcinoma with high NLR values before treatment, concurrent chemoradiotherapy is less effective，which is suggested a poor prognosis. Related Articles | Metrics

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Prognostic value of preoperative albumin/globulin ratio in patients with laryngeal squamous cell carcinoma PANG Zhenwen, GUO Peng, HE Dongsheng, YANG Aifang, HUANG Yufeng, ZENG Xianjie 2019, 11 (5):  407-411.  doi: 10.3969/j.issn.1674-5671.2019.05.09 Abstract ( 505 )   PDF (819KB) ( 258 )   Save Objective To investigate the prognostic value of preoperative albumin/globulin ratio （AGR） in the laryngeal squamous cell carcinoma patients after surgical resection. Methods The clinical data of 147 patients with laryngeal squamous cell carcinoma who underwent laryngeal cancer surgery in department of head and neck surgery of Guangxi Medical University Cancer Hospital from January 2008 to December 2017 were retrospectively analyzed. The cutoff value of AGR was calculated by X-Tile 3.6.1 software，and patients were divided into high AGR group（AGR>1.31，n=98） and low AGR group（AGR≤1.31，n=49） according to the cutoff value. The clinical pathological factors and overall surrival were compared between the two groups. Results  There were significant differences in the difference of tumor location classification，T stage，N stage and clinical stage between the two group patients（P<0.05）.  The 3，5 years overall survival rate of high AGR group were 88.5% and 82.4%，while the 3，5 years overall survival rate of low AGR group were 70.1% and 56.4%. The difference of the overall surrival curve between the two groups was statistically significant（χ2=16.639，P＜0.001）. Univariate Cox regression revealed that smoke，tumor location classification，histological grade，T stage，N stage，clinical stage and AGR level were correlated with patients′ overall survival （P<0.05）. After adjusting for potential confounding factors，Cox regression showed that AGR≤1.31 was an independent risk factor，which affected the overall survival of patients（HR=2.098，95%CI：1.142-3.856，P=0.017）. Conclusion AGR has a relationship with the prognosis of laryngeal sqllamous cell carcinoma and the prognosis of patient with low AGR is poor，which can be used as an indicator of prognosis. Related Articles | Metrics

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Ultrasound guided fusion virtual CT navigation radiofrequency therapy for high risk sites of primary liver cancer PENG Ning, TAO Yiwen, HENG Songqing, YANG Hong, SHANG Liming, XIAO Kaiyin 2019, 11 (5):  412-415.  doi: 10.3969/j.issn.1674-5671.2019.05.10 Abstract ( 326 )   PDF (1131KB) ( 253 )   Save Objective To evaluate the value of real-time ultrasound guided fusion and virtual CT guided radiofrequency ablation （RFA） in the treatment of primary liver cancer in high-risk sites. Methods Clinical data of 80 patients with high-risk primary liver cancer who were treated in the First Affiliated Hospital of Guangxi Medical University from January 2017 to June 2018 were collected prospectively and divided into real-time ultrasound guided fusion virtual CT navigation radiofrequency ablation treatment group （observation group，n=42） and ultrasound guided treatment group（control group，n=38）. The localization rate，ablation rate，compli-cations，and recurrence rate were compared between the two groups. Results The initial success rate and the two ablation rates of the observation group were higher than those of the control group （P<0.05），but the recurrence rate was lower than that of the control group（2.38% vs 21.05%，P=0.011）. There was no significant difference in the incidence of complications between the two groups（2.38% vs 5.26%，P=0.602）. Conclusion High-risk primary liver cancer ultrasound guided fusion virtual CT navigation radiofrequency ablation is better than ultrasound guided therapy. Related Articles | Metrics

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Expression of AKR1B10 protein in papillary thyroid carcinoma tissue and its clinical significance PENG Xuhong, LI Jia, WANG Yu, ZHANG Jian, LUO Weihao, ZHU Lepan, HU Zhen, WU Haiyan, LI Yanlan, DUAN Lili 2019, 11 (5):  416-420.  doi: 10.3969/j.issn.1674-5671.2019.05.11 Abstract ( 429 )   PDF (2300KB) ( 240 )   Save Objective To investigate the expression of aldo-keto reductase family 1B10（AKR1B10） in papillary thyroid carcinoma （PTC） and its clinical significance. Methods Pathological specimens of 237 patients with thyroid nodules diagnosed by ultrasound from the First People's Hospital of Chenzhou from January 2013 to July 2015 were collected. Western blot was used to detect the expression of AKR1B10 protein in normal thyroid tissues and PTC tissues，immunohistochemistry was used to detect the expression of AKR1B10 protein in different thyroid pathological tissues. Results Western blot results showed that the  expression  of AKR1B10 protein in PTC tissues was higher than normal thyroid tissues（0.82±0.10 vs 0.22±0.07，t=2.702，P=0.011）. Immunohistochemistry showed that AKR1B10 was highly expressed in PTC tissues and had the highest postive expression rate（79.5%） in different tissues. AKR1B10 expression level was positively correlated with lymph node metastasis（P=0.007）. Conclusion AKR1B10 is highly expressed in PTC tissues and positively correlated with lymph node metastasis. Related Articles | Metrics

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Application of personalized volumetric modulated arc therapy collimator angle optimization in cervical cancer radiotherapy MING Shufeng, TONG Guangwu, TONG Xiaoguang, HUANG Weibin, WU Zhiquan 2019, 11 (5):  421-425.  doi: 10.3969/j.issn.1674-5671.2019.05.12 Abstract ( 345 )   PDF (809KB) ( 293 )   Save Objective To explore the application effect of personalized volumetric modulated arc therapy collimator angle optimization in cervical cancer radiotherapy. Methods Ten patients with cervical cancer were selected and VMATapt and VMAT20 plans were designed. The VMATapt was randomly divided into three section arcs. According to the projection of the PTV at different gantry angles and based on the minimum field sizes，the optimal segmentation mode and the optimal collimator angle for each section of the arc were calculated in the VMATapt plan. The VMAT20 was planned for a conventional design with a collimator angle of 20°. Compared the averaged Jaw size，the dosimetric parameters of the target area and endangered organs，the machine units（MUs），and the average dose of 2 cm outside the target area（PTV+2 cm） between the two plans. Results Compared with the VMAT20 plan，the averaged Jaw size of VMATapt plan was smaller［（20.2±0.9） cm2 vs （18.5±0.7） cm2，P<0.001］，homogeneity index was better［（0.06±0.01） vs （0.05±0.02），P=0.001］，MUs and PTV+2 cm were lower（P<0.05）. However，there were no significant differences in PTV parameters，organ parameters，and conformal index value between the two plans（P>0.05）. Conclusion VMATapt plan can meet the prescription dose requirements of patients with cervical cancer by using the personalized volumetric modulated arc therapy collimator angle optimization method，and improve the target uniformity and reduce the machine units and PTV+2 cm outside the target area compared to the VMAT20 plan，which can be used for cervical cancer VMAT design. Related Articles | Metrics

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Correlation of psychological distress with self-care efficacy in patients with gastrointestinal tumors CHEN Yanrong, WEI Cuiling, ZHANG Liyuan, QIN Ting 2019, 11 (5):  426-430.  doi: 10.3969/j.issn.1674-5671.2019.05.13 Abstract ( 289 )   PDF (854KB) ( 158 )   Save Objective To investigate the relationship between psychological distress and self-care efficacy in patients with gastroin-testinal tumors. Methods A total of 225 patients diagnosed with gastrointestinal tumors in our hospital were selected. Distress thermometer, hospital anxiety and depression scale and health promotion strategy scale were used to evaluate patients' psychological pain, anxiety and depression and self-care efficacy，Pearson correlation and structural equation model were used to analyze their correlation. Results The average score of psychological distress was（3.15±2.51） in 225 patients with gastrointestinal tumor，of 131 patients （58.22%） suffered psychological distress. The average score of anxiety was （6.02±3.97），of which 172 patients（76.44%） were showing anxiety. The average score of depression was （5.34±4.14）， of which 182 patients （80.89%） were depressed. The total score of self-care efficacy was（94.36±23.63）， and psychological distress，anxiety and depression were negatively correlated with self-care efficacy，self-decompression，self-decision-making and positive attitude （P<0.01）. The structural equation model analysis showed that psychological distress had a direct effect on self-care efficacy， and the standardized path coefficient was -0.186 （standard error=0.093，P=0.002）. Conclusion The incidence of psychological distress，anxiety and depression is higher in patients with gastrointestinal tumors，and the more serious the psychological distress，anxiety and depression，the worse the self-management efficacy. Related Articles | Metrics

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Quality of life in advanced ovarian cancer patients undergoing hyperthermic intraperitoneal chemotherapy QIU Liyu, LI Ni, WU Guili 2019, 11 (5):  430-433.  doi: 10.3969/j.issn.1674-5671.2019.05.14 Abstract ( 347 )   PDF (798KB) ( 213 )   Save Objective To investigate the quality of life of advanced ovarian cancer patients undergoing hyperthermic intraperitoneal chemotherapy（HIPEC）. Methods Three hundred patients with advanced ovarian cancer who received HIPEC were investigated by general data questionnaire，quality of life core questionnaire（QLQ-C30），ovarian cancer treatment function assessment scale（FACT-O），self-perceived burden scale（SPBS），pain number assessment scale（NRS） and social support assessment scale（SSRS）. Results The QLQ-C30 score of the patients with advanced ovarian cancer who underwent HIPEC was（66.28±17.72），the FACT-O score was（94.07±8.80），the SPBS score was（39.82±7.61），the NRS score was（5.68±1.24），and the SSRS score was（23.96±6.57）. Pearson correlation analysis showed that self-perceived burden， social support， and pain were correlated with the quality of life. Conclusion Patients with advanced ovarian cancer undergoing HIPEC have low quality of life，which correlated with self-perception burden，pain，and social support. Related Articles | Metrics

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Research progress of long non-coding RNA GAPLINC and tumor YANG Mingjin, XU Yi, LIN Xuan, HUANG Lining, SUN Cheng, LI Xiang, LU Yuxuan, CUI Yunfu 2019, 11 (5):  434-437.  doi: 10.3969/j.issn.1674-5671.2019.05.15 Abstract ( 302 )   PDF (750KB) ( 267 )   Save Related Articles | Metrics

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Research progress of miR-34a and tumor HUANG Jiaming, ZHU Mingming 2019, 11 (5):  438-442.  doi: 10.3969/j.issn.1674-5671.2019.05.16 Abstract ( 320 )   PDF (762KB) ( 251 )   Save Related Articles | Metrics

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Research advances on microtubules and its targeted agents in apoptosis of osteosarcoma cells HAN Le, XU Biao 2019, 11 (5):  442-446.  doi: 10.3969/j.issn.1674-5671.2019.05.17 Abstract ( 339 )   PDF (756KB) ( 308 )   Save Related Articles | Metrics