Abstract:

Objective　To observe the effects of the Fufang Huazhuo Jiangqi traditional Chinese medicine preparation on a mouse model of precancerous esophageal lesions. Methods Mice were treated with 4-nitro quinoline-oxide（4-NQO）to induce formation of precancerous esophageal lesions，and the animals were distributed into normal group，model group，positive control group，prevention group，treatment group Esophageal epithelial tissue morphology was examined by light microscopy at 14 weeks of treatment，and by electron microscopy at the end of the experiment. Results The model group showed greater cell volume， cell number，and nuclear size at 14 weeks，confirming the successful induction of precancerous esophageal lesions. Malignant transformation occurred in none of the animals in the normal group，80% of animals in the model group，21.4% in the positive control group，5.6% in the prevention group，and 12.5% in the treatment group. At the end of the experiment，animals in the model group showed intercellular broadening，desmosomes，nuclear pycnosis，and chromatin edges. Fewer pathological changes were observed in the positive control，prevention and treatment groups. Conclusion The Fufang Huazhuo Jiangqi prescription may significantly protect against malignant transformation of precancerous esophageal injury in a mouse model. It may therefore be useful for preventing esophageal carcinoma in humans.

Key words: Ｅsophageal neoplasm, Esophageal precancerous, Fufang Huazhuo Jiangqi prescription, pathomorphology, Intervention