Abstract: Objective To investigate the effect of tumor infiltrating lymphocytes(TIL) stimulated by frozen tumor vaccine on melanoma stage Ⅲ.Methods 42 patients with melanoma stage Ⅲ were divided randomly into treatment group(TIL+IL-2,n=22) and control group(IL-2,n=20).All the patients in both groups were treated for 2 months.The overall survival(OS),relapse-free survival(RFS) and side effect of two groups were compared.Results The OS and RFS showed no significant difference between the treatment group and control group(P>0.05).The RFS and OS from the patients with only one invaded lymph node in treatment group improved significantly than that in control group(all P<0.05).All patients in both groups were well tolerated to the treatments.Conclusions TIL+IL-2 can significantly improve the OS and RFS of patients with melanoma stage Ⅲ.The treatment effect on the patient with only one invaded lymph node is better than that with more than one invaded lymph node.

Key words: Melanoma, Tumor infiltrating lymphocytes, Interleukin-2, Treatment