Abstract:

Objective  To investigate the inhibitory effect of KU004 in human breast cancer BT474 nude mouse xenograft model.Methods 60 female BALB/c nude mice were inoculated with human breast cancer BT474 cells，and 40 with tumor growth to 100 -160 mm³ were randomly divided into 5 groups（n=8）；they were gavaged with 40，80，or 120 mg/kg KU004；80 mg/kg lapatinib or 0.5% hydroxypropyl methylcellulose solution respectively，once a day for 21 days. Before and 1，4，7，11，14，17，21 days after the adminis-tration，tumor volume and weight were measured in all 5 groups，and relative tumor growth rate（T/C） and tumor inhibition rate （IR） were calculated. Results  Human breast cancer BT474 nude mouse xenograft model was successfully established. The tumor weight in lapatinib， high-， medium- and low-dose KU004 groups had little change，but there was no significant difference when compared with the model group（P＞0.05）. The TV and RTV of each drug-administered group were significantly lower than that in the model group （P＜0.01），while there was no significant difference between the lapatinib group and the KU004 medium-dose group （P>0.05）. The T/C in lapatinib group， high-，mid-，and low-dose KU004 group  gradually decreased，and T/C was 15.6%，6.9%，11.4%，and 32.7% at 21 days，and IR was 85.0%，95.0%，91.0%，and 67.0%，respectively. Conclusions KU004 in high-，mid-，and low dose can inhibit the tumor growth of human breast cancer BT474 xenograft model，and the nude mice can tolerate well.

Key words: Breast neoplasms, KU004, Breast cancer BT474 cells, Xenograft, Nude mouse, Animal model