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    25 October 2018, Volume 10 Issue 5 Previous Issue    Next Issue
    Analysis of poor prognosis leaded by CCL18 mediated chemokine network in tumor microenvironment  in ovarian cancer patients
    Tang Yuqi,Yu Hongjing,Shi Lijun,ZouYupeng,Feng Yan,Wang Qi
    2018, 10 (5):  345-351.  doi: 10.3969/j.issn.1674-5671.2018.05.01
    Abstract ( 307 )   PDF (1247KB) ( 1011 )   Save

    Objective To investigate the relationship between serum CC-chemokine ligand 18(CCL18) and the prognosis in ovarian cancer patients,and the regulatory effect of serum CCL18 on chemokines and receptors in ovarian cancer microenvironment,and explore the mechanisms of CCL18 in promoting tumor growth and metastasis. Methods Serum CCL18 were examined in 320 cases of ovarian cancer,150 cases of benign pelvic tumor,and 100 cases of healthy women using flow cytometry microspheres. The relation-ship between CCL18 gene expression and prognosis of ovarian cancer patients was analyzed from Gene Expression Omnibus(GEO) and The Cancer Genome Atlas(TCGA). Mice subcutaneous tumor model with ovarian cancer cell lines SKOV3-GFP-CCL18 which over-expresses CCL18 was established. The expression of chemokines and receptors in transplanted tumors were detected by real-time quantitative PCR (qRT-PCR). Results The expression level of CCL18  was (238.04±93.59) ng/mL in ovarian cancer patients, which was significantly higher than in benign pelvic tumor patients(94.36±59.17) ng/mL and healthy women(31.68±26.10) ng/mL(P<0.001). High expression of CCL18 was correlated with poor progression-free survival. Median PFS was 15 months in CCL18 high expression patients,which was significantly lower than 18.2 months in CCL18 low expression patients(HR=1.25,95%CI:1.08~1.44,P=0.003). CCL18 activated XCL1-XCR1,XCL2-XCR1,CCL2-CCR2,CCL11-CCR3,CCL17-CCR4,CXCL9-CXCR3,CXCL11-CXCR3,CXCL12-CXCR4 chemokine-receptor axes in the ovarian cancer microenvironment,and inhibited CXCL1-CXCR2,CXCL6-CXCR2,CXCL8-CXCR2,CCL5-CCR1,CCL5-CCR5,CCL27-CCR10,and CCL28-CCR10 chemokine-receptor axis. Conclusions CCL18 activates the expression of metastatic chemokine-receptors XCL1-XCR1,XCL2-XCR1,CCL2-CCR2,CCL17-CCR4,and down-regulates the expression of CCL5-CCR5 and CCL27-CCR10,CCL28-CCR10,which can cause poor prognosis.

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    KU004 inhibits tumor growth in human breast cancer BT474 nude mouse xenograft model
    Sun Lan,Li Jiachun,Li Yingguang,Guo Qingming,Wang Zhenzhong
    2018, 10 (5):  352-355.  doi: 10.3969/j.issn.1674-5671.2018.05.02
    Abstract ( 325 )   PDF (822KB) ( 613 )   Save

    Objective  To investigate the inhibitory effect of KU004 in human breast cancer BT474 nude mouse xenograft model.Methods 60 female BALB/c nude mice were inoculated with human breast cancer BT474 cells,and 40 with tumor growth to 100 -160 mm3 were randomly divided into 5 groups(n=8);they were gavaged with 40,80,or 120 mg/kg KU004;80 mg/kg lapatinib or 0.5% hydroxypropyl methylcellulose solution respectively,once a day for 21 days. Before and 1,4,7,11,14,17,21 days after the adminis-tration,tumor volume and weight were measured in all 5 groups,and relative tumor growth rate(T/C) and tumor inhibition rate (IR) were calculated. Results  Human breast cancer BT474 nude mouse xenograft model was successfully established. The tumor weight in lapatinib, high-, medium- and low-dose KU004 groups had little change,but there was no significant difference when compared with the model group(P>0.05). The TV and RTV of each drug-administered group were significantly lower than that in the model group (P<0.01),while there was no significant difference between the lapatinib group and the KU004 medium-dose group (P>0.05). The T/C in lapatinib group, high-,mid-,and low-dose KU004 group  gradually decreased,and T/C was 15.6%,6.9%,11.4%,and 32.7% at 21 days,and IR was 85.0%,95.0%,91.0%,and 67.0%,respectively. Conclusions KU004 in high-,mid-,and low dose can inhibit the tumor growth of human breast cancer BT474 xenograft model,and the nude mice can tolerate well.

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    Analysis of the alternative splicing events of AFB1-induced liver cancer in rats based on RNA-seq
    Tan Ni,Cao Ji,Yang Chun,Zheng Haiping,Ou Chao
    2018, 10 (5):  356-360.  doi: 10.3969/j.issn.1674-5671.2018.05.03
    Abstract ( 291 )   PDF (831KB) ( 783 )   Save

    Objective To explore the mechanism of aflatoxin B1(AFB1)-induced liver cancer in rats and the alternative splicing events that blocked the molecular mechanism of liver cancer. Methods Model of AFB1-induced liver cancer in rats was constructed, total RNA from rat liver tissue was extracted,and Illumina HiSeq 2000 sequencing platform was used to sequence RNA-seq  in rat liver tissue. Using rat genomic data as a reference,332 known reference genes with multiple  alternative splicing variants were currently included in the study. The rat genomic alternative splicing events were identified and analyzed, and biological process (BP)enrichment analysis for GO categorization of alternative spliced genes with differentially expressed was conducted. Results Compared with the control group,18 alternative splicing patterns of genes in the non-cancer group showed a big difference,and mainly enriched in the stimulatory response function. Compared with the control group,37 alternative splicing patterns of genes in the cancer group were different,and mainly enriched in the stimulation response and cell proliferation regulation function. Compared with the non-cancer group,the alternative splicing patterns of 32 genes in the cancer group were significantly different,mainly enriched in the cell proliferation regulation function. In the cancer group,7 splicing patterns of genes involved in cell proliferation regulation were found to be abnormal. The alternative splicing patterns of IgfI,Carm1,and Tcfe2a were significantly different between the cancer group,the non-cancer group,and the control group. Conclusion Alternative splicing changes of IgfI,Carm1,Tcfe2a may play an important role in AFB1-induced liver cancer formation in rats.

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    Clinical efficacy of capecitabine combined with Endostar in the treatment of advanced colon cancer after stent implantation
    Wang Shuaibing,Guo Qian,Wu Zhenming,Liu Jun,Qi Xiuheng
    2018, 10 (5):  360-364.  doi: 10.3969/j.issn.1674-5671.2018.05.04
    Abstract ( 245 )   PDF (917KB) ( 699 )   Save

    Objective To investigate the efficacy and safety of capecitabine combined with recombinant human endostatin(Endostar) in the treatment of patients with advanced colon cancer after stent implantation. Methods A total of 79 patients with advanced colon cancer who were unable to undergo surgical resection and complicated with intestinal obstruction were recruited between  January 2010 and January 2017. Patients  were  divided into  control group(n=40)that received capecitabine  alone or treatment  group(n=39) that received capecitabine with Endostar. The short-term efficacy and adverse reactions were compared between the two groups,and the progression-free survival and overall survival were observed. Results All patients were successfully implanted with intestinal stents to remove the obstruction and completed the chemotherapies. The total response rate of the treatment group was higher than that in the control group(46.15% vs 30.00%),but there was no significant difference(χ2=2.921,P=0.087),and the treatment group showed significantly higher disease control rate than that in the control group(69.23% vs 50.00%,χ2=3.978,P=0.046). The incidence rate of adverse reactions associated with chemotherapy and intestinal stenting was similar between the two groups(P>0.05). Median progression-free survival was 16 months in the treatment  group,significantly longer than 13 months in the control group(P<0.05). Similarly,median overall survival was 25 months in the treatment group,significantly longer than 19 months in the control group(P<0.05). Conclusions Intestinal stenting can effectively relieve intestinal obstruction in patients with colon cancer,and capecitabine combined with Endostar is superior to capecitabine alone.

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    Meta-analysis:the relationship of hepatitis B virus genotypes,subgenotypes and hepato-cellular carcinoma in China
    Wu Linghong,Huang Tianren,Deng Wei,Huang Zhibi,Guo Jiao,Huang Yueying
    2018, 10 (5):  365-370.  doi: 10.3969/j.issn.1674-5671.2018.05.05
    Abstract ( 295 )   PDF (1284KB) ( 724 )   Save

    Objective  To explore the correlation between hepatitis B virus(HBV) genotype and subgenotype and hepatocellular carcinoma in China. Methods A case control study on the correlation between Chinese HBV genotypes,subgenotype and hepato-cellular carcinoma,published from January 2007 to February 2017,was retrieved in a comprehensive retrieval system. The literature was selected and the quality was evaluated and effective data were extracted according to the exclusion criteria. The Meta analysis was analyzed by RevMan 5.3 analysis. Results Finally,37 studies were included in the study of HBV genotype and hepatocellular carcinoma,4,109 cases in case group,15,319 in control group;8 in HBV gene subtype(C1,C2,B2) with hepatocellular carcinoma,931 in case group and 5,793 in control group,and the quality score of the included literature was more than 7. The results of Meta analysis showed that:⑴the risk of HBV C genotype was higher than that of genotype B(OR=2.35,95%CI:1.93-2.87,P<0.001);there was no significant difference in the risk of hepatocellular carcinoma of HBV C and A,D subgenotype(OR=1.25,95%CI:0.20-7.82,P=0.81); ⑵there was no significant difference in the risk of hepatocellular carcinoma of HBV C1 and C2 subgenotype(OR=1.05,95%CI:0.66-1.68,P=0.82),and the risk of hepatocellular carcinoma in HBV C2 subgenotype is stronger than that of B2 subgenotype(OR=1.77,95%CI:1.38-2.27,P<0.001). Conclusions HBV genotype C has higher risk of liver cancer than other major genotypes. There is no significant difference in the risk of hepatocellular carcinoma in HBV C1 and C2 subgenotype,but the risk of hepatocellular carcinoma is higher in HBV C2 subgenotype than that in B2 subgenotype.

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    Systematic review of transarterial embolization for hepatocellular adenoma
    Jia Rongrong,Huo Rongrui,Ge Dexiang,Luo Chengpiao,Zhang Chunyan
    2018, 10 (5):  371-376.  doi: 10.3969/j.issn.1674-5671.2018.05.06
    Abstract ( 219 )   PDF (843KB) ( 693 )   Save

    Objective To evaluate the role of transarterial embolization(TAE) in bleeding and non-bleeding hepatocellular adenoma(HCA). Methods Relevant studies for the transarterial embolization in HCA from the databases EMBASE,PubMed and Web of Science were searched from January 2000 to December 2017. Literature screening,data extraction and quality assessment were conducted according to inclusion and exclusion criteria. Results A total of 21 studies were eligible for inclusion in the study,including 1,481 patients with HCA,of whom 148 were treated with TAE. Of the 148 patients who underwent TAE, 93 had HCA bleeding and 55 had non-bleeding HCA,of which 45 were non-acute TAE. A total of 110 tumors met the revised RECIST criteria,with the proportion of complete and partially reduced tumors being 10.0% (11/110))and 71.8%(79/110), respectively. A total of 72 patients could avoid hepatectomy,including 23 cases of bleeding HCA and 49 cases of non-bleeding HCA. Of the 45 patients who were treated with non-acute TAE,43 did not require further surgery. No mortality or serious adverse side effects occurred during hospitalization. Conclusions TAE may be considered as a safe treatment for patients with HCA. For patients with non-bleeding HCA,TAE may be a reasonable alternative treatment besides hepatectomy.

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    Influence factors of radiation-induced lung injury after intensity-modulated radiotherapy in elderly (above 80 years) patients with esophageal squamous carcinoma
    Ji Jingna,Wang Xiaomin,Zhao Fujun,Wang Yuekun,Sheng Zhaojie,Chen Long
    2018, 10 (5):  377-380.  doi: 10.3969/j.issn.1674-5671.2018.05.07
    Abstract ( 346 )   PDF (862KB) ( 851 )   Save

    Objective To analyze the influence factors of radiation-induced lung injury(RILI) after intensity-modulated radiotherapy in elderly(above 80 years)patients with esophageal squamous carcinoma. Methods Clinical data of 94 elderly (above 80 years) patients with esophageal squamous cell carcinoma who underwent intensity-modulated radiotherapy from May 2014 to October 2016 were analyzed. Influencing factors of RILI were identified using multivariate logistic regression analysis. Results Among the 94 patients,the incidence of RILI was 24.5%(23/94). Univariate analysis identified the following risk factors: smoking, underlying lung diseases, and lung V5P<0.05). Multivariate analysis identified lung V5≤55% as a protective factor for RILI(OR=0.072,95%CI:0.017-0.308,P<0.001),and underlying lung disease was a risk factor(OR=11.289,95%CI:2.186-58.312,P=0.004). Conclusions The incidence of  radiation-induced lung injury is high after intensity-modulated radiotherapy in elderly (above 80 years) patients with esophageal squamous carcinoma.Underlying lung diseases increases incidence risk of RILI,and lung V5≤55% can effectively reduce the occurrence of RILI.

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    iagnostic value of serum CA125 combined with NLR and PLR for endometrial cancer
    Hu Ningning,Nan Fangfang,Dou Qianru,Liu Qin,Zhang Yingzi
    2018, 10 (5):  381-384.  doi: 10.3969/j.issn.1674-5671.2018.05.08
    Abstract ( 263 )   PDF (880KB) ( 750 )   Save

    Objective To explore the diagnostic value of carbohydrate antigen 125(CA125) serum level combined with neutrophil/lymphocyte ratio(NLR) and platelet/lymphocyte ratio (PLR) for endometrial cancer(EC). Methods Medical records were collected and analyzed for 291 patients,179 cases of endometrial cancer and 112 cases of normal endometrium. The receiver operating characteristic curve (ROC) and logistic regression equation were used to analyze the diagnostic value of CA125,NLR and PLR ffor EC,and the relationship between CA125  serum level and clinicopathological characteristics were analyzed. Results The CA125 seum level,NLR and PLR were(32.73±58.43) U/mL, 2.40±1.21 and 157.89±70.87 in the endometrial cancer group,which were significantly higher than that in the normal endometrium group(16.06±13.47) U/mL,1.87±0.54 and 138.88±43.07(P<0.05). Correlation analysis showed that CA125  was significantly positive correlated with NLR(r=0.171,P=0.003) and PLR(r=0.146,P=0.013),and PLR showed a significant positive correlation with NLR(r=0.595,P<0.001). The AUCs for diagnosing EC:CA125,0.706;NLR,0.648;PLR,0.555;Combination,0.792;while the AUC of three combined showed significantly higher diagnostic accuracy than either serum CA125,NLR and PLR alone(P<0.05). Conclusion The accuracy of serum CA125 combined with NLR and PLR for the diagnosis of endometrial cancer is higher than that of single test.

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    Incidence and mortality of colorectal cancer in areas with cancer registration in Guangxi,2014
    Tang Yanping,Cao Ji,Rong Minhua,Li Qiulin,Ge Lianying,Hu Bangli,Yu Jiahua
    2018, 10 (5):  385-389.  doi: 10.3969/j.issn.1674-5671.2018.05.09
    Abstract ( 307 )   PDF (870KB) ( 708 )   Save

    Objective  To analyze the incidence and mortality of colorectal cancer in Guangxi in 2014 based on areas with cancer registry reporting. Methods A total of 11 cancer registries of Guangxi reported cancer data for 2014,and 9 registries were found to conform to data quality criteria of the National Central Cancer Registry(NCCR). Data from these 9 registries were analyzed to determine incidence and mortality of colorectal cancer stratified by areas(urban/rural),sex,and age group,and cumulative incidence/mortality rates,proportions,and truncated incidence/mortality rates were calculated. The Chinese population census in 2000 and Segi′s population were used for age-standardized incidence/mortality rates. Results  The  number of new colorectal cancer cases was 1,716(males 1,039,females 677) from 9 registry areas. The crude incidence of colorectal cancer in Guangxi cancer registration areas was 24.74/105,age-standardized incidence rates was 19.68/105 based on the Chinese standard population(ASR China)and 19.37/105 based on the world standard population(ASR world),and the colorectal cancer incidence rate of males was significantly higher than females(28.73%  vs 20.40%,χ2=48.33,P<0.001). The incidence of colorectal cancer and ASR China were 36.19/105 and 27.45/105 in urban areas,compared to 19.24/105 and 16.07/105 in rural areas. The total number of colorectal cancer-related deaths was 827(males 518,females 309). Crude mortality associated with colorectal cancer in Guangxi cancer registration areas was 11.93/105, age-standardized mortality rate was 9.06/105 based on the Chinese standard population(ASR China)and 9.00/105 based on the world standard population (ASR world),and the colorectal cancer mortality rate of males was significantly higher than females(14.32%  vs 9.31%,χ2=36.400,P<0.001). Mortality associated with colorectal cancer and ASR China were 17.63/105 and 13.21/105 in urban areas,compared to 9.18/105 and 7.13/105 in rural areas. Conclusions In Guangxi,incidence of colorectal cancer and associated mortality are obviously higher in males than in females. The incidence and mortality of colorectal cancer are higher in urban areas than in rural areas.Urban male over 45 years old is the the main target population for cancer prevention and treatment.

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    Application value of real-time shear wave elastography in distinguish of molecular subtypes in breast invasive ductal carcinoma 
    Wang Yaoqin,Tang Lina,Liu Naxiang,Chen Yijie,Lin Ruoxuan,Zhong Zhaoming
    2018, 10 (5):  390-392.  doi: 10.3969/j.issn.1674-5671.2018.05.10
    Abstract ( 267 )   PDF (660KB) ( 790 )   Save

    Objective To explore the relationship between the ultrasonic real-time shear wave elastography and molecular subtypes of breast invasive ductal carcinoma. Methods A total of 137 patients(139 nodules) with breast invasive ductal carcinoma were confirmed by operation or gross needle biopsy. Ultrasonic real-time shear wave elastography was performed,and the relationship between the maximum elastic modulus value and molecular subtypes were analyzed. Results The maximum value of shear wave elas-tic modulus in Luminal type A,Luminal type B,HER-2 overexpression type,triple negative type were getting larger. The maximum value of shear wave elastic modulus differed significantly between the triple negative type and the Luminal A,B types(P<0.05).Spearman correlation analysis showed that the maximum value of ultrasonic real-time shear wave elastic modulus was significantly correlated with molecular subtypes(r=0.510,P=0.003). Conclusion The maximum value of ultrasonic real-time shear wave elastic modulus have a certain value in distinguish of molecular subtypes in breast invasive ductal carcinoma.

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    Effect of celecoxib on radiochemotherapy sensitivity of nasopharyngeal carcinoma
    Wang Xiangping,Zhan Yongzi,Mao Yan,Chen Hao,Liang Ting,Huang Changjie
    2018, 10 (5):  393-396.  doi: 10.3969/j.issn.1674-5671.2018.05.11
    Abstract ( 275 )   PDF (815KB) ( 646 )   Save

    Objective To investigate the effect and safety of celecoxib on radiochemotherapy sensitivity in patients with nasopharyn-geal carcinoma(NPC). Methods Patients  with nasopharyngeal carcinoma(n=73) were enrolled from  December 2013 to December 2017 and randomly divided into two groups:the control group(n=35) received concurrent chemoradiotherapy,while the experimental group(n=38) received the same chemoradiotherapy in combination with celecoxib. The short-term efficacy and safety were compared between the two groups. Results The complete response rate in the experimental group was significantly higher than that in the control group(57.9% vs  22.9%,χ2=7.849,P=0.005),and the adverse reactions such as gastrointestinal reactions, radiation injury,renal dysfunction,and myelosuppression were  similar between the two groups(P>0.05). Conclusions Celecoxib combined with radiochem-otherapy show better complete response rate and good short-term efficacy for nasopharyngeal carcinoma,and it is well tolerated.

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    A comparison of different types of anaesthesia for total knee arthroplasty for malignant tumors 
    Wang Qiang,Huang Wen,Zhou Weixiao,Rong Xiangbin
    2018, 10 (5):  397-399.  doi: 10.3969/j.issn.1674-5671.2018.05.12
    Abstract ( 190 )   PDF (678KB) ( 637 )   Save
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    Research progress on the role of TIM-3-Gal-9 pathway in virus associated diseases
    Chen Zhizhong,Zhao Wei,Xiao Yangyang,Qing Jilin
    2018, 10 (5):  403-408.  doi: 10.3969/j.issn.1674-5671.2018.05.14
    Abstract ( 255 )   PDF (739KB) ( 866 )   Save
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    Research progress in the relationship between exosome and multiple myeloma
    He Yupeng,Nong Feng,Fu Dongdong,Tang Yujin,Liu Jia
    2018, 10 (5):  409-411.  doi: 10.3969/j.issn.1674-5671.2018.05.15
    Abstract ( 313 )   PDF (666KB) ( 745 )   Save
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    Advances in the use of opioids for cancer pain
    Huang Yu,Jing Ren,Pan Linghui
    2018, 10 (5):  412-415.  doi: 10.3969/j.issn.1674-5671.2018.05.16
    Abstract ( 285 )   PDF (798KB) ( 779 )   Save
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    Primary pulmonary artery sarcoma misdiagnosed as pulmonary embolism: a case report
    Guan Qingyan, He Xiuchao, Yu Yingchun, Yang Nan, Guo Ying
    2018, 10 (5):  415-416.  doi: 10.3969/j.issn.1674-5671.2018.05.17
    Abstract ( 235 )   PDF (841KB) ( 570 )   Save
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    Iodin-125 seed implantation for lung metastasis from hemangiopericytoma: a case report
    Wang Yuanyuan,He Bin,Zhang Lili
    2018, 10 (5):  417-418.  doi: 10.3969/j.issn.1674-5671.2018.05.18
    Abstract ( 174 )   PDF (967KB) ( 376 )   Save
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