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26 December 2016, Volume 8 Issue 6 Previous Issue    Next Issue

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Synergistic effects of aspirin and caffeine for preventing colorectal cancer Liu Hanyang, Song Jun, Zhou Yan, Liu Kun, Tang Liming 2016, 8 (6):  337-343.  doi: 10.3969/j.issn.1674-5671.2016.06.01 Abstract ( 348 )   PDF (2088KB) ( 474 )   Save Objective This study aimed to investigate the synergistic effects of low-dose aspirin and caffeine for preventing colorectal cancer. Methods The colorectal cell lines Caco-2,SW-480,HT-29,and HCT-116 were exposed to low-dose aspirin alone,caffeine alone or both agents,then cell viability was measured using the cell counting kit-8 and extent of apoptosis was measured using flow cytometry. Expression of relevant factors(p53,PTEN,EGFR,TNF-α) was measured using real-time PCR. Drug-withdrawal experiments were conducted. Results Treating cell lines with both caffeine and aspirin inhibited cell proliferation and accelerated early apoptosis significantly more than treating cells with either agent alone. Western blotting showed that treating with aspirin alone caffeine alone and with the combination of aspirin and caffeine up-regulated p53,Cyt-C and caspase-9/-3 expression, and the difference of treatment with the combination of aspirin and caffeine was the most significant(P<0.05). The level of cell vitality were improved,when the p53-specific inhibitor pifithrin-α were added. In drug-withdrawal experiments, caffeine treatment prolonged p53 up-regulation and inhibition of proliferation. Conclusion Aspirin and caffeine synergistically inhibit proliferation and promote apoptosis of colorectal cancer cells. In addition, maintaining caffeine can prolong growth inhibition after aspirin withdrawal. Related Articles | Metrics

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DDX39 single-nucleotide polymorphism and genetic susceptibility to hepatocellular carcinoma in clusters of families with liver cancer in Fusui County, Guangxi Ren Ai'hua,Liao Yan,Wei Liujun,Zhao Ruiqiang,Wei Feifei,Xie Yu'an 2016, 8 (6):  344-348.  doi: 10.3969/j.issn.1674-5671.2016.06.02 Abstract ( 313 )   PDF (1236KB) ( 237 )   Save Objective To investigate the relationship between DDX39 rs12461754 single-nucleotide polymorphism (SNP) and family clustering genetic susceptibility to hepatocellular carcinoma(HCC) in Guangxi. Methods Genotypes and alleles at SNP rs12461754 were determined using time-of-flight mass spectrometry in 20 HCC family groups(77 cases) and 10 normal control family groups(35 cases) in Fusui County. Results Risk of HCC for individuals with the A allele of DDX39 rs12461754 was 0.407-fold (95% CI: 0.125-1.326, P=0.136) the risk for individuals with the G allele among normal control groups, while risk of HCC for individuals with the A allele was 0.981-fold(95% CI: 0.298-3.326, P=0.976) the risk for individuals with the G allele among unaffected members of HCC families. The frequencies of A and G alleles were similar between patients and unaffected individuals of HCC families(P>0.05). Frequencies of AA,AG,and GG genotypes did not differ significantly between patients in HCC families and unaffected individuals in HCC families or normal control families. Conclusion Alleles at the DDX39 SNP rs12461754 site may not correlate with family clustering of HCC in Guangxi. Related Articles | Metrics

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Optimizing construction of a nasopharyngeal cancer CNE-2R cell stably expressing XAF1 2016, 8 (6):  349-353.  doi: DOI:10.3969/j.issn.1674-5671.2016.06.03 Abstract ( 137 )   PDF (1596KB) ( 460 )   Save Objective To examine methods for constructing a nasopharyngeal cancer cell line(CNE-2R) stably expressing (X-linked inhibitor of apoptosis associated factor 1)XAF1. Method CNE-2R cells were infected with lentivirus encoding XAF1 as well as a green fluorescent protein reporter. Cells were infected at multiplicities of infection(MOI) of 50 or 100 without polybrene, or at an MOI of 100 in the presence of polybrene(5 μg/mL). Expression of XAF1 mRNA and protein was verified using quantitative RT-PCR and Western blotting. Results Faint fluorescence was detected in 60% of CNE-2R cells after transfection with lentivirus at an MOI of 50. A slightly higher percentage(70%) of CNE-2R cells showed weak fluorescence after transfection with lentivirus at an MOI of 100. When cells were infected at an MOI of 100 in the presence of polybrene, 90% of CNE-2R cells showed strong fluorescence. Stably transfected cell lines were selected using puromycin. Conclusion The efficiency with which XAF1-expressing lentivirus can infect CNE-2R cells is closely related to the state of the cells, MOI, and polybrene concentration. Addition of appropriate concentrations of polybrene to lentivirus can increase efficiency of infection. Stably transfected cell lines can be selected with puromycin.   Related Articles | Metrics

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Anaplastic lymphoma kinase gene expression and its clinical significance in hepatocellular carcinoma Jia Shouwei,Shao Qiong,Wu Xiaoyan,Cao Li,Long Zijun,Shao Jianyong,Huang Hongbing 2016, 8 (6):  354-359.  doi: 10.3969/j.issn.1674-5671.2016.06.04 Abstract ( 267 )   PDF (1341KB) ( 401 )   Save Objective To examine the status and clinical significance of anaplastic lymphoma kinase(ALK) gene alterations in hepatocellular carcinoma(HCC) patients. Methods We detected ALK gene expression in HCC tissues using fluorescent in situ hybridization,and we assessed the correlation of ALK gene expression with clinicopathologic outcomes and prognosis in HCC. Results ALK gene translocation was not observed in any HCC cases in the present study. In contrast, gain in ALK gene copy number (ALK-CNG)was detected in 28 HCC patients, and this copy number gain did not positively correlate with age,sex,AJCC stage,recurrence or Child-Pugh classification(P>0.05). Copy number gain did positively correlate with poorer prognosis in HCC(P=0.048),especially among patients with stage Ⅲ/Ⅳ disease(P=0.007). Univariate and multivariate Cox proportional hazards regression analysis suggested that ALK-CNG was an independent prognostic indicator for patients with HCC(P=0.046). Conclusion  ALK-CNG, but not translocation of ALK,is present in HCC and may predict unfavorable prognosis.   Related Articles | Metrics

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Expression and clinical significance of both hypoxia-inducible factor(HIF)-1α and CD44 in gastric cancer Huang Mingwei,Wu Liucheng,Qin Yuzhou,Li Zhao,Zhang Meng,Su Di,Chen Jiansi 2016, 8 (6):  360-364.  doi: 10.3969/j.issn.1674-5671.2016.06.05 Abstract ( 328 )   PDF (1413KB) ( 234 )   Save  Objective To investigate the relationship of expression of both HIF-1α and CD44 with clinicopathological features and outcomes in gastric cancer patients. Methods Tissue from 184 gastric cancer cases who underwent R0 resection were analyzed by immunohistochemistry to examine the expression of HIF-1α and CD44. Potential relationships of expression of HIF-1α and CD44 with clinicopathological features and prognosis were investigated. Results HIF-1α was expressed in 102/184(55.4%)of gastric cancer patients,and CD44 was expressed in 116/184(62.4%). HIF-1α and CD44 expression correlated with tumor invasion depth and lymphatic metastasis(P<0.05),and CD44 expression also correlated with tumor size(P<0.05). Spearman rank correlation analysis indicated that HIF-1α expression positively correlated with CD44 expression(r=0.71,P=0.003). Cox multivariate regression analysis indicated that tumor invasion depth, lymphatic metastasis, HIF-1α and CD44 expression were independent prognostic factors in gastric cancer patients(P<0.05).Conclusions HIF-1α expression positively correlated with CD44 expression in gastric cancer patients,and expression of both proteins was related to poor prognosis. Related Articles | Metrics

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ROS1 expression in pulmonary adenocarcinoma and its clinical significance Liang Xiaoqin,Wang Zhuo|,Chang Shoufeng,Zhang Xiaoling,Chen Cheng,Wang Jinsui,Su Qinjun 2016, 8 (6):  364-368.  doi: DOI:10.3969/j.issn.1674-5671.2016.06.06 Abstract ( 220 )   PDF (1113KB) ( 265 )   Save  Objective To detect the expression of ROS1 protein and its potential clinical significance in patients with pulmonary adenocarcinoma. Methods Formalin-fixed,paraffin-embedded tissue microarrays prepared from 332 patients with pulmonary adenocarcinoma and 55 samples of adjacent normal lung tissue were analyzed for ROS1 expression using immunohistochemistry. Possible relationships were analyzed between ROS1 expression and clinicopathologic features or EGFR/ALK protein expression, as well as between protein expression and postoperative recurrence. Results The rate of ROS1 expression was 6% in pulmonary adenocarcinoma tissues and 0 in adjacent normal lung tissue(P<0.05). ROS1 expression did not correlate with age or gender, but it did correlate significantly with smoking history and clinical stage(P<0.05). Disease-free survival was 42 months in ROS1-negative patients and 19 months in ROS1-positive patients(P<0.05). Rate of recurrence at 1 year was 30% in ROS1-negative patients and 13.5% in ROS1-positive patients(P<0.05). Conclusion Expression of ROS1 protein is low in pulmonary adenocarcinoma. ROS1 may be a prognostic factor that predicts risk of recurrence and metastasis of lung adenocarcinoma. Screening for ROS1 expression may help guide clinical treatment.   Related Articles | Metrics

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Correlation between cellular immunity and WHO classification of newly diagnosed glioma Deng Teng,Mo Ligen,Chen Hainan,Zeng Xianji 2016, 8 (6):  369-372.  doi: 10.3969/j.issn.1674-5671.2016.06.07 Abstract ( 227 )   PDF (912KB) ( 572 )   Save Objective To investigate the cellular immunity status of glioma patients and their correlation with the WHO classification of gliomas. Methods The clinical data of 92 patients with newly diagnosed patients analyzed retrospectively.Preoperative cellular immunity was measured by flow cytometry and WHO classification of gliomas were confirmed by postoperative pathology. The correlation between cellular immunity and WHO classification of glioma was analyzed by Spearman's rank correlation coefficient. Results No statistically significant differences were observed concerning CD3+,CD4+,CD8+,CD4+/CD8+,CD16+CD56+orCD19+(P<0.05).No significant correlation between cellular immunity and WHO classification of gliomas were observed by Spearman's rank correlation coefficient(P<0.05). Conclusions There is no significant correlation between patient's cellular immunity and WHO classification of gliomas. Preoperative cellular immunity test can not differentiate low grade glioma from high grade glioma.       Related Articles | Metrics

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Risk factors for the presence of multiple drug-resistant bacteria in patients infected with pneumonia in hospital Lin Zhen,Huang Qianqian,Qin Jin'ai,Song Yingjiao,Wen Shan,Gan Yongjiang,Li Chunfeng,Wei Zhongsheng 2016, 8 (6):  372-377.  doi: 10.3969/j.issn.1674-5671.2016.06.08 Abstract ( 211 )   PDF (1095KB) ( 171 )   Save Objective To investigate risk factors associated with multiple drug-resistant(MDR) bacteria in patients infected with pneumonia in hospital. Methods A total of 518 nosocomial cases of pneumonia at seven grade 3 hospitals in Guangxi were followed up from April to October,2013. Of these cases,268 cases were found to be positive for MDR bacteria and 250 were found to be negative. Potential differences between MDR bacteria-positive and -negative groups were analyzed in order to identify potential risk factors of MDR infection. Results MDR bacteria were detected in 51.74% of nosocomial pneumonia infections,with the prevalence of MDR highest in pneumonia cases contracted in the intensive care unit. Logistic analysis identified low hemoglobin concentration and the use of two types of antibiotics within 1 month as independent risk factors for the presence of MDR bacteria in nosocomial pneumonia infections(P<0.05). Conclusions Risk factors for occurrence of MDR bacteria in nosocomial cases of pneumonia should be managed more effectively in order to decrease the hospital infection rate; for example,antibiotics should be used properly.     Related Articles | Metrics

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Clinical pathological analysis of endometrial metastasis of breast cancer Meng Lingxin,Meng Qin,Zhou Dandan,Wang Chuanyan,Li Bingcheng,Li Xin,Yao Zhigang 2016, 8 (6):  378-382.  doi: 10.3969/j.issn.1674-5671.2016.06.09 Abstract ( 329 )   PDF (1865KB) ( 477 )   Save Objective To investigate the clinical pathological characteristics, treatment and prognosis of endometrial metastasis of breast cancer,which is very rare. Methods This retrospective study analyzed the clinical data of 3 patients with endometrial metastasis of breast cancer treated at our hospital,and relevant literature was reviewed. Results Two patients had breast-infiltrating lobular carcinoma,while one patient had invasive ductal carcinoma. Endometrial metastatic lesions had a median diameter of 4.5 cm (range,3-8 cm). Cancer cells from endometrial metastatic lesions were round or oval under the microscope,had dense cytoplasm and showed pleomorphism;tumor cells were arranged in tubular,beam or funicular structures,with lobular structures in some parts. Primary tumors and endometrial metastatic lesions in all three patients were positive for ER,PR and Ki-67,while they showed variable staining for CerbB-2,GCDFP-15 and E-cadherin. Primary tumors and endometrial metastatic lesions were positive for p120 and CK7,but negative for CK5/6,SMA,CD10 and S-100. Conclusions Breast cancer patients with endometrial metastasis are rare and have poor prognosis and short survival time. Diagnosis is based mainly on medical history,pathomorphology and immunophenotype.Staining for ER,PR,CK5/6,CK7,CerbB-2 and GCDFP-15 plays a role in differential diagnosis of uterine endometrial carcinoma in primary and metastatic cancer.   Related Articles | Metrics

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Epidemiology of HBV infection and viral genotypes from liver cancer prevention screening in Guigang City, Guangxi Province Chen Qihuang,Deng Wei,Cao Ji,Cai Zhengmin,Ge Lianying 2016, 8 (6):  382-386.  doi: 10.3969/j.issn.1674-5671.2016.06.10 Abstract ( 205 )   PDF (1057KB) ( 202 )   Save Objective To analyze data on hepatitis B virus(HBV) infection and viral genotypes collected as part of liver cancer prevention screening in Guigang City,Guangxi Province. Methods Data were collected from residents of Guigang City who agreed to participate in liver cancer screening,and 1,989 residents at high risk of liver cancer were randomly selected for quantitative detection of HBV DNA. HBV carriers with an HBV load of≥1×103 copies were subjected to analysis by PCR-RFLP to determine HBV genotypes. Result The average rate of HBsAg positivity in the study population was 11.99%,and the rate was significantly higher in males than in females(P<0.01). The rate differed significantly among different age groups(P<0.01),declining gradually with increasing age. A total of 755 HBV carriers at high risk of liver cancer were identified to have at least 1×103 HBV copies. Of these 755,6(0.79%) were infected with genotype A,337(44.64%) with genotype B,293(38.81%) with genotype C,and 18 (2.39%)with genotype D. The genotype in 101 carriers(13.37%)was untypable. These two genotypes did not differ significantly between men and women or among the different age groups. Conclusion The HBV infection rate is high in the three districts of Guigang City, with HBV genotypes B and C predominating. The rate of early detection of liver cancer is relatively low. Technology and program management should be improved to enhance the efficacy of cancer screening, early detection and treatment.       Related Articles | Metrics

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Microcystin content of different water sources in a region of Guangxi showing high incidence of liver cancer Li Kezhi,Deng Wei,Li Yunxi,Li Jilin,Zhang Chunyan,Ren Jingjing,Wu Hanghang 2016, 8 (6):  387-390.  doi: 10.3969/j.issn.1674-5671.2016.06.11 Abstract ( 194 )   PDF (974KB) ( 353 )   Save Objective To investigate microcystin contamination in water bodies that feed the water supply system of Fusui County, which shows a high incidence of liver cancer in Guangxi Province. Method Water sources in 11 villages and tapwater in 28 villages were sampled. Temperature and pH were recorded during sampling,and microcystin was assayed using ELISA. Result Source water and tapwater did not differ significantly in temperature or pH. Average microcystin concentration was(15.64±2.08) ng/L in source water,and(14.42±2.28) ng/L in tapwater. These values were not significantly different. Microcystin concentration in source water varied with the trend: river>subterranean river>well water. Microcystin concentration in tapwater varied with the trend: reservoir water>well river>subterranean river>river water. However, these trends were not statistically significant. The concentration of microcystin was 16.69 ng/L in river water, which was the highest concentration of all water sources tested. Average microcystin concentration was(13.90±2.62) ng/L in treated tapwater and(14.71±2.09) ng/L in untreated tapwater. These values were not significantly different. Conclusion Microcystin concentration did not exceed the WHO-recommended limit of 0.001 mg/L in any sample tested.       Related Articles | Metrics

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Application of video recording of laparoscopic gastrointestinal surgery in postgraduate clinical teaching Qin Yuzhou,Wu Liucheng,Huang Mingwei|,Yang Yang,Li Zhao,Chen Jiansi 2016, 8 (6):  390-392.  doi: 10.3969/j.issn.1674-5671.2016.06.12 Abstract ( 240 )   PDF (898KB) ( 260 )   Save Related Articles | Metrics

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Progress in diagnosis of adrenal tumor Xian Jing,Luo Zuojie 2016, 8 (6):  392-396.  doi: 10.3969/j.issn.1674-5671.2016.06.13 Abstract ( 275 )   PDF (1050KB) ( 357 )   Save Related Articles | Metrics

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Long non-coding RNA MALAT1 and cervical cancer Shi Danli,Wang Jiali,Tang Yanping,Pang Xiaofen,Mo Lingzhao 2016, 8 (6):  397-400.  doi: 10.3969/j.issn.1674-5671.2016.06.14 Abstract ( 223 )   PDF (888KB) ( 491 )   Save Related Articles | Metrics

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Biological activities of lactic acid in cancer: influences on tumor behaviors Bai Xiaofang,Zhang Junping 2016, 8 (6):  401-403.  doi: 10.3969/j.issn.1674-5671.2016.06.15 Abstract ( 206 )   PDF (803KB) ( 551 )   Save Related Articles | Metrics

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Dual-Energy CT imaging applications in lung cancer: diagnosis and effect evaluation Fu Danhui,Lai Shaolv,Su Danke 2016, 8 (6):  404-407.  doi: 10.3969/j.issn.1674-5671.2016.06.16 Abstract ( 196 )   PDF (989KB) ( 288 )   Save Related Articles | Metrics