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中国癌症防治杂志

中国癌症防治杂志 ›› 2016, Vol. 8 ›› Issue (5): 269-272.doi: 10.3969/j.issn.1674-5671.2016.05.01

• 基础研究 •    下一篇

牛磺酸对裸鼠乳腺癌模型肿瘤生长的影响及其机制

  

  1. 广西医科大学公共卫生学院营养与食品卫生学教研室
  • 出版日期:2016-10-25 发布日期:2016-11-14
  • 通讯作者: 郭松超 2433164518@qq.com
  • 基金资助:

    广西科技基础条件平台建设项目资助（14 -91-02）

Effects of taurine on tumor growth in a nude mouse breast cancer model

  • Online:2016-10-25 Published:2016-11-14

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被引次数

3

摘要：

目的 探讨牛磺酸对裸鼠乳腺癌模型肿瘤生长的影响及机制。方法 10只SPF级5周龄裸鼠接种MDA-MB-231乳腺癌细胞后随机分为牛磺酸干预组和对照组，每组5只。牛磺酸干预组给予添加5%的牛磺酸饮水，对照组给予正常无菌饮水。测量两组小鼠肿瘤体积和肿瘤质量，采用蛋白免疫印迹法检测雌激素受体（estrogen receptor，ER）、孕激素受体（progesterone receptor，PR）、人类表皮生长因子受体2（human epidemic growth factor receptor 2，HER-2）和鸟苷三磷酸酶激活蛋白（guanosine triphosphatase activating protein，p21ras）表达量的变化。结果 实验结束时所有裸鼠均存活，裸鼠乳腺癌模型造模成功。牛磺酸对裸鼠乳腺癌模型肿瘤的抑制率为41.6%。相对于对照组，牛磺酸干预组裸鼠肿瘤组织中ER表达量提高了134%（P<0.05），PR表达量提高了47.5%（P<0.05），HER-2表达量降低了67%（P<0.05），p21ras表达量降低了62.2%（P<0.01）。结论 牛磺酸可抑制裸鼠乳腺癌模型肿瘤生长，其机制可能与上调ER、PR和下调HER-2、p21ras有关。

关键词: 乳腺肿瘤, 牛磺酸, 抗肿瘤, 裸鼠, 雌激素受体, 孕激素受体, 人类表皮生长因子受体2, 鸟苷三磷酸酶激活蛋白

Abstract:

Objective To study the effect of taurine on the humanized nude mouse model of triple-negative breast cancer. Methods Five-week-old SPF level nude mice（n=10） were inoculated with MDA-MB-231 breast cancer cells and randomly divided into one group that received normal drinking water and another group that received water containing 5% taurine（n=5 animals per group）. Tumor volume and weight were examined after treatment, and Western blotting was used to assess expression of estrogen receptor （ER）, progesterone receptor（PR），human epidermal growth factor receptor 2 （HER-2）and guanosine triphosphatase-activating protein p21ras. Results All mice survived until the end of the experiment，and all tumors grew. Compared to control mice, mice given taurine had tumors with 41.6% smaller weight and volume（P<0.05），134% higher expression of ER（P<0.05）, 47.5% higher expression of PR （P<0.05），67% lower expression of HER-2（P<0.05）and 62.2% lower expression of p21ras（P<0.01）. Conclusion Taurine inhibits tumor growth by up-regulating expression of ER and PR and down-regulating expression of HER-2 and p21ras.

Key words: Breast neoplasms, Taurine, Antitumor, Nude mice, Estrogen receptor, Progesterone receptor, Human epidemic growth factor receptor 2, Guanosine triphosphatase activating protein