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中国癌症防治杂志

中国癌症防治杂志 ›› 2020, Vol. 12 ›› Issue (4): 415-420.doi: 10.3969/j.issn.1674-5671.2020.04.09

• 基础研究 • 上一篇    下一篇

ST6GalⅠ抑制TNFR1表达调控肾透明细胞癌凋亡

  

  1. 新疆医科大学第一附属医院泌尿外科
  • 出版日期:2020-08-25 发布日期:2020-09-01
  • 通讯作者: 拜合提亚·阿扎提 E-mail： bhty08@sina.cn
  • 基金资助:
    吴阶平医学基金会临床科研专项资助基金（320.6750.19094-43）

ST6GalⅠinhibits the expression of TNFR1 to regulate the apoptosis of renal clear cell carcinoma

  • Online:2020-08-25 Published:2020-09-01

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摘要：

目的 ST6GalⅠ和TNFR1在肾透明细胞癌细胞中的表达。构建ST6GalⅠ差异表达肾透明细胞癌细胞株，采用流式细胞术检测细胞凋亡水平，并分析ST6GalⅠ和TNFR1表达的相关性，qRT-PCR和Western blot检测差异表达ST6GalⅠ细胞中TNFR1的表达，采用流式细胞术检测同时差异表达ST6GalⅠ和TNFR1的A498和Caki-1细胞的凋亡水平。结果 与ST6GalⅠ在5种肾透明细胞癌细胞中均高表达（P＜0.05）；抑制ST6GalⅠ表达后A498细胞凋亡率升高（P＜0.01），Caki-1细胞过表达ST6GalⅠ后细胞凋亡率降低（P＜0.001）。TNFR1 mRNA和蛋白在5种肾透明细胞癌细胞株中均低表达，且与ST6GalⅠmRNA和蛋白表达呈负相关（r=-0.9667，-0.9928，均P<0.01）；抑制ST6GalⅠ表达 TNFR1表达增加（P＜0.001），而ST6GalⅠ过表达TNFR1表达降低（P＜0.001）；A498细胞同时抑制ST6GalⅠ和 TNFR1表达后细胞凋亡率低于单独抑制ST6GalⅠ（P＜0.01），Caki-1细胞同时过表达ST6GalⅠ和TNFR1后细胞凋亡率高于单独抑制ST6GalⅠ（P＜0.01）。结论 ST6GalⅠ可通过抑制TNFR1表达而抑制肾透明细胞癌的凋亡。

关键词: 肾透明细胞癌, ST6GalⅠ, TNFR1, 凋亡

Abstract:

Objective To investigate the effect of ST6GalⅠ on the apoptosis of renal clear cell carcinoma cells and itspossible mechanism. Methods The expression of ST6GalⅠ and TNFR1 in renal clear cell carcinoma cell lines were detected byqRT-PCR and Western blot. The renal clear cell carcinoma cell lines with different expression of ST6GalⅠ were constructed，and the apoptosis level was detected by flow cytometry，and the correlation between the expression of ST6GalⅠ and TNFR1was analyzed，the expression of TNFR1 in the differentially expressed ST6GalⅠcells were detected by qRT-PCR and Westernblot，and the apoptosis level of A498 and Caki-1 cells that differentially expressed ST6GalⅠand TNFR1 were detected byflow cytometry. Results ST6GalⅠ was highly expressed in 5 renal clear cell carcinoma cell lines（P＜0.05）. The apoptosis

rate of A498 cells increased after ST6Gal Ⅰ was inhibited （P＜0.01），while the apoptosis rate of Caki-1 cells reducedfor the overexpression of ST6GalⅠ（P<0.001）. TNFR1 mRNA and protein were low-expressed in 5 renal clear cell cancer celllines，and negatively correlated with ST6GalⅠmRNA and protein expression（r=-0.9667，-0.9928，all P<0.01）. The expressionof TNFR1 increased when the expression of ST6Gal Ⅰ（P<0.001） was inhibited，while the expression of TNFR1 decreased forthe overexpression of ST6GalⅠ（P<0.001）. The apoptosis rate of A498 cells after inhibition of both ST6GalⅠand TNFR1expression was lower than that of ST6GalⅠalone （P<0.01），and the apoptosis rate of Caki-1 cells after overexpression ofboth ST6GalⅠand TNFR1 was higher than that of ST6GalⅠalone （P<0.01）. Conclusions ST6GalⅠcan inhibit the apoptosis ofrenal clear cell carcinoma cell lines by inhibiting the expression of TNFR1.

Key words: Renal clear cell carcinoma, ST6GalⅠ, TNFR1, Apoptosis

中图分类号: 

  • R737.11