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中国癌症防治杂志

中国癌症防治杂志 ›› 2020, Vol. 12 ›› Issue (1): 57-62.doi: 10.3969/j.issn.1674-5671.2020.01.10

• 临床研究 • 上一篇    下一篇

线粒体钙离子摄入蛋白1在肾透明细胞癌组织中的表达及其临床意义

  

  1. 延安大学医学院；空军军医大学唐都医院疼痛科；徐州医科大学附属淮海医院普通外科；延安大学附属医院检验科
  • 出版日期:2020-02-25 发布日期:2020-03-10
  • 通讯作者: 刘晓斌 E-mail：lxb2411817@163.com。 张召辉 E-mail：zzhlyn@163.com

Expression and clinical significance of mitochondrial calcium uptake 1 in renal clear cell carcinoma tissues#br#

  • Online:2020-02-25 Published:2020-03-10

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摘要： 目的 探讨线粒体钙离子摄入蛋白1（mitochondrial calcium uptake 1，MICU1）在肾透明细胞癌（renal clear cell carcinoma，RCCC）组织中的表达及其临床意义。方法 以2016年1月—2018年12月于延安大学附属医院行根治性肾切除术的30例RCCC患者为研究对象。采用免疫组织化学法检测RCCC组织及癌旁正常组织中MICU1蛋白的表达。采用瞬时转染法分别将MICU1干涉片段和MICU1过表达质粒转染到CAKI-1细胞中（siMICU1组和MICU1组），分别设置相应的阴性对照（siCtrl组和EV组）。采用Western blot检测蛋白表达，MTS实验和EdU实验检测细胞的增殖能力。收集TCGA数据库中517例RCCC组织转录组数据，分析MICU1 mRNA表达与RCCC患者临床病理特征及预后的关系。结果 MICU1蛋白在RCCC组织中表达水平低于癌旁正常组织（P<0.001）。成功构建过表达MICU1细胞和干涉MICU1细胞，MTS实验和EdU实验显示，MICU1组的细胞增殖能力低于EV组（P<0.05），siMICU1组的细胞增殖能力高于siCtrl组（P<0.05）。TCGA数据库分析结果显示，MICU1表达与性别、TNM分期有关（P<0.05）；MICU1低表达患者生存率低于高表达患者（χ2=19.290，P＜0.001）；多因素Cox回归显示，MICU1低表达是影响RCCC患者总生存期的独立危险因素（HR=1.641， 95%CI：1.191~2.261，P=0.002）。结论　MICU1在RCCC组织中呈低表达，且可促进RCCC细胞增殖。

关键词: 肾透明细胞癌, 线粒体钙离子摄入蛋白1, 增殖, 预后

Abstract: Objective  To investigate the expression and clinical significance of mitochondrial calcium uptake 1（MICU1） in patients with renal clear cell carcinoma（RCCC）. Methods Cases of thirty patients with RCCC，who underwent radical nephrectomy in Yan'an University Affiliated Hospital from January 2016 to December 2018，were investigated. Immunohistochemical method was used to detect the expression of MICU1 protein in RCCC cancer tissues and adjacent normal tissues. Transient transfection was used to transfect the MICU1 interference fragment and the MICU1 overexpression plasmid into CAKI-1 cells（siMICU1 group and MICU1 group），respectively，and corresponding negative controls（siCtrl group and EV group） were set. Western blot was used to detect the expression of MICU1 protein，and MTS and EdU assays were used to detect the proliferation of CAKI-1 cells. The transcriptome data of 517 RCCC tissues in TCGA database were collected，and the relationship between MICU1 mRNA expression and clinicopathological characteristics and prognosis of RCCC patients were analyzed. Results The expression of MICU1 protein in RCCC tissues was lower than that in normal adjacent tissues（P<0.001）. The overexpressed MICU1 cells and interfered MICU1 cells were successfully constructed. MTS and EdU assays showed that the cell proliferation ability of the MICU1 group was lower than that of the EV group（P<0.05），and that of the siMICU1 group was higher than that of the siCtrl group（P<0.05）. TCGA database analysis showed that MICU1 expression was related to gender and TNM stage（P<0.05）； the survival rate of patients with low expression of MICU1 was lower than that of patients with high expression（χ2=19.290，P<0.001）； multivariate Cox regression showed that low expression of MICU1 was an independent risk factor affecting the overall survival of RCCC patients（HR=1.641，95%CI：1.191-2.261，P=0.002）. Conclusion MICU1 has a low expression in RCCC tissues and can promote the proliferation of RCCC cancer cells. 

Key words: Renal clear cell carcinoma, MICU1, Proliferation, Prognosis

中图分类号: 

  • R737.11