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中国癌症防治杂志

中国癌症防治杂志 ›› 2022, Vol. 14 ›› Issue (1): 32-37.doi: 10.3969/j.issn.1674-5671.2022.01.06

• 基础研究 • 上一篇    下一篇

氩氦刀冻融治疗对兔VX2肝移植瘤微血管的影响

  

  1. 南方医科大学第一临床医学院中国人民解放军中部战区总医院肿瘤科 
  • 出版日期:2022-02-25 发布日期:2022-03-11
  • 通讯作者: 易峰涛 E-mail：flzxyft@163.com

Effect of argon⁃helium knife cryoablation on microvessels of rabbit VX2 liver transplantation tumors

  • Online:2022-02-25 Published:2022-03-11

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摘要： 目的 探讨氩氦刀冻融治疗对兔VX2肝移植瘤微血管的影响。方法 通过将VX2肿瘤组织肝内注射法构建兔VX2肝移植瘤模型15只，随机分为对照组和不同时间氩氦刀冻融治疗组（包括3 d组、7 d组、15 d组、30 d组），每组3只。采用320排CT增强扫描及组织学HE染色法观察治疗区域肿瘤组织形态变化，通过免疫组化法检测肿瘤微血管密度（microvascular density，MVD）-CD31、MVD-VEGF的表达情况。结果 成功构建15只兔VX2肝移植瘤模型。CT检查结果显示，对照组肿瘤分为周边环状强化区和中间坏死区；冻融治疗组治疗部位血流缺损，呈低密度空洞状，且低密度范围随治疗时间进展逐渐缩小；若冻融不全，可见残留病灶强化。组织学检测结果显示，冻融治疗后形成无细胞结构的坏死区域，伴有炎性细胞反应带，随时间进展炎性细胞逐渐减少，并出现纤维增生带；若冻融不全，治疗区周边可见肿瘤细胞残留。免疫组化检测结果显示，与对照组相比，MVD-CD31、MVD-VEGF表达在冻融3 d组和冻融7 d组明显增加（均P<0.05），在冻融15 d组和冻融30 d组明显降低（均P<0.05）。结论 氩氦刀冻融治疗可以抑制兔VX2肝移植瘤微血管新生，可能是肝脏肿瘤有效的治疗手段。

关键词: 肝移植瘤, 兔VX2瘤, 冻融治疗, 微血管密度, 320排CT, 免疫组化

Abstract: Objective To investigate the effect of argon-helium knife cryoablation on the microvessels of rabbit VX2 liver transplantation tumors. Methods Fifteen rabbit VX2 liver transplantation tumor models were constructed by VX2 tumor tissue intrahepatic injection, and randomly divided into a control group and an argon-helium knife cryoablation group (including a 3 d group, a 7 d group, a 15 d group, and a 30 d group), with 3 rabbits in each group. The morphological changes of tumors in the treated area were observed by 320-row CT enhanced scanning and histological HE staining. The expression of tumor microvascular density(MVD)-CD31 and MVD-VEGF was detected by immunohistochemistry. Results Fifteen rabit VX2 liver transplantation tumor models were constructed successfully. The results of CT showed that the tumors in the control group were divided into a peripheral ring enhancement area and a middle necrotic area. In the cryoablation group, the blood flow defect of the treated site showed a low-density cavity, and the low-density range gradually narrowed with time; if the cryoablation area was incomplete, the residual lesions could be enhanced. The histological results showed that a necrotic area without cell structure was formed after treatment, accompanied by an inflammatory cell response zone, and inflammatory cells gradually decreased with time, and a fibrous hyperplasia zone appeared. If the cryoablation area was incomplete, residual tumor cells could be seen around the treatment area. The results of immunohistochemistry showed that compared with the control group, the expressions of MVD-CD31 and MVD-VEGF increased significantly in the cryoablation 3 d group and 7 d group (all P<0.05), but significantly decreased in the cryoablation 15 d group and 30 d group (all P<0.05). Conclusions The cryoablation with argon-helium knife can inhibit the microangiogenesis of rabbit VX2 liver transplantation tumors, and may provide an effective treatment for liver tumor.

Key words: Liver transplantation tumor, Babbit VX2 tumor, Cryoablation, Microvascular density, 320-row CT, Immunohistochemical staining

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