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Chinese Journal of Oncology Prevention and Treatment ›› 2012, Vol. 4 ›› Issue (2): 136-139.doi: 10.3969/j.issn.1674-5671.2012.02.10

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Expression of CCT subunit γ in aflatoxin B1-induced hepatocarcinogenesis in Wistar rats

  

  • Online:2012-06-25 Published:2012-07-03

Abstract: Objective To investigate the protein expression and possible role of CCT subunit γ in hepatocarcinogenesis induced by aflatoxin B1(AFB1)in rats. Methods A total of 45 Wistar rats were randomly divided into a control group and an AFB1 group,which was injected intraperitoneally with 200 μg/kg AFB1.Liver biopsies were performed on all animals at weeks 13,33 and 53.Animals were sacrificed at week 73 and liver tissues were collected.Protein expression of CCT subunit γ was dynamically examined by immunohistochemistry during different phases of AFB1-induced hepatocarcinogenesis. Results The protein expression levels of the CCT subunit γ were significantly higher in HCC tissues of the AFB1 group(100%,10/10)than in tissues of the control group (30%,3/10). The protein level gradually increased during development of hepatic carcinoma, with the level at week 73 significantly higher than at weeks 13 and 33(P<0.01).At week 73,the level of CCT subunit γ was higher in the AFB1 group than in the control group (P<0.05).The expression level of CCT subunit γ did not differ significantly between the two groups at other times(P>0.05). Conclusion Overexpression of CCT subunit γ may be related to HCC carcinogenesis.

Key words:  Liver neoplasms, CCT subunit γ, Aflatoxin B1, Rat hepatocellular carcinoma;Protein expression